A5016094497- Heat shock proteins research
- Cardiac electrophysiology and arrhythmias
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- thermodynamics and calorimetric analyses
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Nonlinear Dynamics and Pattern Formation
- RNA and protein synthesis mechanisms
- Bioinformatics and Genomic Networks
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Cardiomyopathy and Myosin Studies
- Cardiac Arrhythmias and Treatments
- Gene expression and cancer classification
- Medical Imaging Techniques and Applications
- Protein Structure and Dynamics
- Genetics, Bioinformatics, and Biomedical Research
- Biochemical Analysis and Sensing Techniques
- Erythrocyte Function and Pathophysiology
- Neuroscience and Neural Engineering
- Radiomics and Machine Learning in Medical Imaging
Université de Bourgogne
2010-2021
Inserm
2011-2021
Université Bourgogne Franche-Comté
2016-2021
La Ligue Contre le Cancer
2015-2021
Centre Georges François Leclerc
2015-2020
State University of New York
2020
Centre de recherche Translationnelle en Médecine moléculaire
2011-2020
CHU Dijon Bourgogne
2015
Laboratoire CarMeN
2013
Maastricht University
2006-2012
Exosomes, via heat shock protein 70 (HSP70) expressed in their membrane, are able to interact with the toll-like receptor 2 (TLR2) on myeloid-derived suppressive cells (MDSCs), thereby activating them. We analyzed exosomes from mouse (C57Bl/6) and breast, lung, ovarian cancer patient samples cultured different approaches, including nanoparticle tracking analysis, biolayer interferometry, FACS, electron microscopy. Data were Student's t Mann-Whitney tests. All statistical tests two-sided....
Abstract Escherichia coli is an important experimental, medical and industrial cell factory for recombinant protein production. The inducible lac promoter one of the most commonly used promoters heterologous expression in E. . Isopropyl-β-D-thiogalactoside (IPTG) currently efficient molecular inducer regulating this promoter’s transcriptional activity. However, limitations have been observed large-scale microplate production, including toxicity, cost culture monitoring. Here, we report novel...
APO2L/TRAIL (TNF-related apoptosis-inducing ligand) induces death of tumor cells through two agonist receptors, TRAIL-R1 and TRAIL-R2. We demonstrate here that N-linked glycosylation (N-glyc) plays also an important regulatory role for TRAIL-R1-mediated mouse TRAIL receptor (mTRAIL-R)-mediated apoptosis, but not TRAIL-R2, which is devoid N-glycans. Cells expressing N-glyc-defective mutants TRAIL-R were less sensitive to than their wild-type counterparts. Defective apoptotic signaling by...
First discovered in 1962, heat shock proteins (HSPs) are highly studied with about 35,500 publications on the subject to date. HSPs conserved, function as molecular chaperones for a large panel of "client" and have strong cytoprotective properties. Induced by many different stress signals, they promote cell survival adverse conditions. Therefore, their roles been investigated several conditions pathologies where accumulate, such cancer. Among diverse mammalian HSPs, some members share...
Tumour proliferation is one of the main biological phenotypes limiting cure in oncology. Extensive research being performed to unravel key players this process. To exploit potential published gene expression data, creation a signature for can provide valuable information on tumour status, prognosis and prediction. This will help individualising treatment should result better control, more rapid cost-effective development. From vitro microarray studies, two signatures were compiled. The...
The purpose of this study was to increase the potential dose redistribution by incorporating estimates oxygen heterogeneity within imaging voxels for optimal determination. Cellular tension (pO2) distributions were estimated imaging-size-based solving diffusion–consumption equations around capillaries placed at random locations. linear-quadratic model used determine cell survival in as a function pO2 and dose. distribution across tumour optimized yield minimal after 30 × 2 Gy fractions...
Renaud Seigneuric 1,2 , Jessica Gobbo Pierre Colas 3 Carmen Garrido 1 Heat Shock Proteins and Cancer, INSERM, UMR 866 IFR 100, Faculty of Medicine, 7 Boulevard Jeanne D'Arc, 21000 Dijon, France 2 Université de Bourgogne, CNRS USR 3151, P2I2 Group, Station Biologique, Roscoff, Bretagne, Received: June 22, 2011; Accepted: 24, Published: Correspondence: Seigneuric, email: // Abstract A major endeavour in cancer chemotherapy is to develop agents that specifically target a biomolecule interest....
β-thalassemia major (β-TM) is an inherited hemoglobinopathy caused by a quantitative defect in the synthesis of β-globin chains hemoglobin, leading to accumulation free a-globin that aggregate and cause ineffective erythropoiesis. We have previously demonstrated terminal erythroid maturation requires transient activation caspase-3 chaperone Heat Shock Protein 70 (HSP70) accumulates nucleus protect GATA-1 transcription factor from cleavage. This nuclear HSP70 inhibited human β-TM...
The presence of an inactivating heat shock protein 110 (HSP110) mutation in colorectal cancers has been correlated with excellent prognosis and the ability HSP110 to favor formation tolerogenic (M2-like) macrophages. These clinical experimental results suggest a potentially powerful new strategy against cancer: inhibition HSP110. In this work, as alternative neutralizing antibodies, Nanofitins (scaffold ~7 kDa proteins) targeting were isolated from screening synthetic Nanofitin library,...
Background and Purpose Leptin, an adipokine synthesized by the placenta during pregnancy, has been proposed for management of preterm labour ( PTL ), as it is able to prevent in vitro uterine contractility remodelling associated with onset. Another common feature onset phenotypic switch myometrial smooth muscle cells from a proliferative hypertrophic state. As effects have demonstrated leptin other tissues, we aimed investigate its ability induce proliferation thus maintain quiescence....
The human inducible heat shock protein 70 (hHsp70), which is involved in several major pathologies, including neurodegenerative disorders and cancer, a key molecular chaperone contributes to the proper folding maintenance of large number structures. Despite its role disease, current structural knowledge hHsp70 almost exclusively based on Escherichia coli homolog, DnaK, even though these two proteins only share ~50 % amino acid identity. For first time, we describe complete heterologous...