A5088159118- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- Enzyme Structure and Function
- Virus-based gene therapy research
- Meningioma and schwannoma management
- Head and Neck Surgical Oncology
- Antibiotic Resistance in Bacteria
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Congenital Diaphragmatic Hernia Studies
- Neural dynamics and brain function
- Pancreatic and Hepatic Oncology Research
- Plant Genetic and Mutation Studies
- Neuroendocrine Tumor Research Advances
- Photoreceptor and optogenetics research
- Neurofibromatosis and Schwannoma Cases
- Carcinogens and Genotoxicity Assessment
- CAR-T cell therapy research
- Retinal Development and Disorders
- Neuroscience and Neural Engineering
- Pituitary Gland Disorders and Treatments
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
Hospital Universitario De Cabueñes
2024
Newcastle University
2017-2022
Centro Nacional de Biotecnología
2013-2021
Consejo Superior de Investigaciones Científicas
2015
Lentiviral vectors are used in laboratories around the world for vivo and ex delivery of gene therapies, increasingly clinical investigation as well preclinical applications. The third-generation lentiviral vector system has many advantages, including high packaging capacity, stable expression both dividing post-mitotic cells, low immunogenicity recipient organism. Yet, manufacture these is challenging, especially at titers required direct use vivo, further challenges presented by process...
Abstract Patients with advanced gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) have impaired nutritional and physical performance due to the cancer pathophysiology its treatment. The NUTRIGETNE study sought characterize status of patients GEP-NENs in Spain. This is a cross-sectional that included receiving active oncological had complete examination, anthropometry, bioelectrical impedance, dynamometry, laboratory analysis, comprehensive risk assessment. Malnutrition was defined...
To expand the use of human retinal organoids from induced pluripotent stem cells (iPSCs) as an in vitro model retina for assessing gene therapy treatments, it is essential to establish efficient transduction. date, targeted transduction photoreceptor-like with adeno-associated virus (AAV) vectors has had varied degrees success, which we have looked improve this study.
Bacillus subtilis diadenylate cyclase DisA converts two ATPs into c-di-AMP, but this activity is suppressed upon interaction with sites of DNA damage. forms a rapid moving focus that pauses induction damage during spore development. We report pausing, however, was not observed in the absence RecO mediator or RecA recombinase, suggesting binds to recombination intermediates formed by concert RecO. DisA, which physically interacts RecA, found reduce its ATPase without competing for nucleotides...
The DNA damage checkpoint protein DisA and the branch migration translocase RecG are implicated in preservation of genome integrity reviving haploid Bacillus subtilis spores. synthesizes essential cyclic 3', 5'-diadenosine monophosphate (c‑di-AMP) second messenger such synthesis is suppressed upon replication perturbation. In vitro, c-di-AMP when binds structures that mimic stalled or reversed forks (gapped Holliday junctions [HJ]). RecG, which does not form a stable complex with DisA,...
DNA lesions that impede fork progression cause replisome stalling and threaten genome stability. Bacillus subtilis RecA, at a lesion-containing gap, interacts with facilitates DisA pausing these branched intermediates. Paused suppresses its synthesis of the essential c-di-AMP messenger. The RuvAB-RecU resolvasome branch migrates resolves formed Holliday junctions (HJ). We show prevents degradation. DisA, which RuvB, binds structures, reduces RuvAB DNA-dependent ATPase activity. pre-bound to...
<title>Abstract</title> Purpose Studies focused on the effects of sellar and/or perisellar (S/PS) meningiomas pituitary function are scarce. We evaluate clinical characteristics, therapeutic outcomes, and that S/PS their treatments have function. Methods A multicenter retrospective study patients with attended in 20 tertiary Spanish centers. Results 171 patients, 129 (75.4%) females, median age 61.5 [51–71] years were analyzed. At presentation, 118 (69.1%) had visual disturbance, 66 (38.6%)...