A5002087684- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Vector-Borne Animal Diseases
- Invertebrate Immune Response Mechanisms
- Antimicrobial Peptides and Activities
- Immune cells in cancer
- Neuroendocrine regulation and behavior
- Mycobacterium research and diagnosis
- Malaria Research and Control
- Vector-borne infectious diseases
- Microbial infections and disease research
- Lipid Membrane Structure and Behavior
- Radiopharmaceutical Chemistry and Applications
- Glycosylation and Glycoproteins Research
- Viral Infections and Vectors
- Hemoglobin structure and function
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2005-2022
Instituto de Biotecnología de León
2009-2013
Universidad Nacional de Colombia
2003-2004
Fundación Instituto de Inmunología de Colombia
2003-2004
Hospital Universitario Príncipe de Asturias
1993
Universidad de Alcalá
1993
ABSTRACT Haemophilus parasuis is a colonizer of healthy piglets and the etiological agent Glässer's disease. Differences in virulence among strains H. have been widely observed. In order to explore host-pathogen interaction, snatch-farrowed colostrum-deprived were intranasally infected with 4 : reference virulent strain Nagasaki, nonvirulent SW114, field IT29205 (from systemic lesion previous challenge), F9 nasal cavity piglet). At different times after infection, two animals each group...
Recently moderate-virulence classical swine fever virus (CSFV) strains have been proven capable of generating postnatal persistent infection (PI), defined by the maintenance viremia and inability to generate CSFV-specific immune responses in animals. These animals also showed a type I interferon blockade absence clinical signs. In this study, we assessed generated 7-week-old CSFV PI wild boars after with African (ASFV). The were divided two groups infected ASFV. Group A comprised who...
The population of malaria-causing parasites is characterized by great genetic diversity. Knowledge the polymorphism generation mechanism a central issue for developing effective vaccines against malaria and understanding parasite structure. Plasmodium vivax diversity has been explained in terms two major factors: natural selection intragenic recombination. A modular organization was found within P. Duffy binding protein present work. Four Colombian isolates have identical sequences to...
CD163 is a member of the family proteins with scavenger receptor cysteine-rich domains, whose expression restricted to monocytes and macrophages. It functions as for haemoglobin/haptoglobin complexes it has also been implicated in regulation inflammatory processes. Treatment monocytes/macrophages phorbol esters, LPS or cross-linking Fc gamma receptors, causes shedding from cell surface by protease-dependent mechanism. The level soluble (sCD163) serum other body fluids considered useful...
In this report, we describe the characterization of a new monoclonal antibody, named 4H5CR4, against porcine CD9. Its use in combination with antibodies to CD4, CD8α, and 2E3 allows distinguish at least five main CD4+ T cell subsets. Analysis on these subsets CD45RA, CD27, CD29, CD95, CCR7, SLA-DR markers depicts progressive model development. 2E3+ CD8α− CD9− cells are differentiated population naïve cells, whereas 2E3− CD8α+CD9+ CD8α+ display phenotypic features central effector memory...
Gammadelta T lymphocytes have a heterodimeric complex formed by the association of gamma and delta chains as receptor. Proliferation this lymphocyte population has been observed, when infection several pathogens such Mycobacterium tuberculosis Plasmodium spp. occurs. The New World Monkey Aotus nancymaae become very good experimental model for immunological physiopathological study these infectious agents. A. gamma-variable region was characterized from peripheral blood samples using cDNA...
CLEC12A has been proposed as a suitable target for delivering antigen to dendritic cells (DCs) enhance vaccine efficacy both in human and mouse. In this study, we have characterized the porcine homologue of (poCLEC12A). Using new monoclonal antibodies (mAb), raised against its ectodomain, poCLEC12A was found be expressed on alveolar macrophages, blood conventional type 1 2 DCs plasmacytoid DCs, but not monocytes, T cells, B or NK contrast murine homologues. Western blot analysis showed that...
Abstract: Functional and structural analyses of major histocompatibility complex (MHC) class I molecules the Aotus genus are necessary to validate it as a solid animal model for biomedical research. We thus isolated, cloned sequenced exons 2 3 from three species ( A. nancymaae , nigriceps vociferans ). found 24 sequences, which divided into two different groups (Ao‐g1 Ao‐g2). A further sequence was identified processed pseudogene (Aona‐PS2). Both evolution variability showed that Ao‐g1 Ao‐g2...
Vasoactive intestinal peptide (VIP) has been shown to stimulate adenylyl cyclase activity in human endometrial membranes. The effect was dependent on the time and temperature of incubation as well concentration membrane proteins medium. In presence 1 μM GTP, half-maximal stimulation observed at 25.0 ± 7.0 nM VIP, whereas maximal (at VIP)corresponded an increase about 140% with respect basal values (7.5 0.6 pmol cyclic AMP/min/mg protein). However, obtained helodermin (1 μM) that increased by...
The inhibitory receptor CD200R1 and its paired activating CD200R1L are involved in the regulation of myeloid cell immune responses. aim this study was to analyze their distribution, by cytokines, function porcine monocyte subsets. We had previously observed that genes can generate different protein isoforms through alternative mRNA splicing, therefore study, we explored diversity transcripts subsets, described several new splicing variants both CD200R1L, some which could be expressed on...
The CD200R family comprises a group of paired receptors that can modulate the activation immune cells. They are expressed both on myeloid cells and lymphocyte subsets. Here we report expression these porcine B is tightly regulated, being mainly mature inhibitory CD200R1 and/or its splicing variant CD200R1X2, either in combination or not with activating receptor CD200R1L, upregulated sIgM+ effector/memory cells, tends to decline thereafter as progress plasmablasts switch Ig isotype. naïve...