A5031326040- Xenotransplantation and immune response
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Cell Adhesion Molecules Research
- Electrospun Nanofibers in Biomedical Applications
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- Viral Infections and Immunology Research
- Cardiac Ischemia and Reperfusion
- Glycosylation and Glycoproteins Research
- Renal Transplantation Outcomes and Treatments
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Cardiac Fibrosis and Remodeling
- ATP Synthase and ATPases Research
- Viral Infectious Diseases and Gene Expression in Insects
- Transplantation: Methods and Outcomes
- Peptidase Inhibition and Analysis
- Cardiomyopathy and Myosin Studies
Complexo Hospitalario Universitario A Coruña
2013-2024
Universidade da Coruña
2003-2024
Centro de Investigación en Red en Enfermedades Cardiovasculares
2018-2024
Centro de Investigación Biomédica en Red
2018-2024
Instituto de Investigación Biomédica de A Coruña
2014-2024
Instituto de Salud Carlos III
2024
Servicio Gallego de Salud
2018-2022
British Heart Foundation
2012-2016
King's College London
2012-2016
St George's Hospital
2016
Background— After myocardial ischemia, extracellular matrix (ECM) deposition occurs at the site of focal injury and border region. Methods Results— We have applied a novel proteomic method for analysis ECM in cardiovascular tissues to porcine model ischemia/reperfusion injury. proteins were sequentially extracted identified by liquid chromatography tandem mass spectrometry. For first time, such as cartilage intermediate layer protein 1, matrilin-4, adipocyte enhancer binding collagen...
Abstract Human breast and pancreatic adenocarcinomas are tumors of ductal epithelial cell origin as such produce express on their surface polymorphic mucin encoded by the MUC 1 gene. We have previously reported that tumor-specific cytotoxic T cells derived from patients bearing recognize specific epitopes polypeptide core. This recognition was not MHC-restricted because highly repetitive sequence core, which allows simultaneous many identical epitopes, cross-linking aggregation TCR...
Background: Remodeling of the extracellular matrix (ECM) is a hallmark heart failure (HF). Our previous analysis secretome murine cardiac fibroblasts returned ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5) as one most abundant proteases. cleaves chondroitin sulfate proteoglycans such versican. The contribution its substrate versican to HF unknown. Methods: Versican remodeling was assessed in mice lacking catalytic domain (Adamts5 ΔCat ). Proteomics applied study...
Epithelial cell mucin encoded by the MUC-1 gene is overexpressed and aberrantly glycosylated on pancreatic, breast, ovarian cancers as well multiple myelomas. It recognized patients' Ab T cells derived from tumor-draining lymph nodes. The recognition not MHC restricted specific for an epitope previously localized to immunodominant tandem repeat region of native molecule. In search possible MHC-restricted epitopes in same region, we synthesized a panel overlapping, nine-amino acid long...
Abstract The mAb 2A10 recognizes a 120-kDa protein with sequence homology to the human CD163 and whose expression is restricted cells of porcine monocyte/macrophage lineage. While most tissue macrophages express high levels Ag, bone marrow subset blood monocytes are negative for this marker. percentage 2A10+ ranges between 5–50% depending on donor. phenotypic analysis indicates that these more similar mature than 2A10− monocytes. higher swine histocompatibility leukocyte Ag II, CD16,...
Myocardial fibrosis is a feature of many cardiac diseases. We used proteomics to profile glycoproteins in the human extracellular matrix (ECM).Atrial specimens were analyzed by mass spectrometry after extraction ECM proteins and enrichment for or glycopeptides.ECM-related identified left right atrial appendages from same patients. Several known glycosylation sites confirmed. In addition, putative novel detected. On glycoproteins, peptides small leucine-rich proteoglycan decorin consistently...
Introduction and objectives Mitochondrial pyruvate carrier (MPC) mediates the entry of into mitochondria, determining whether is incorporated Krebs cycle or metabolized in cytosol. In heart failure (HF), a large amount to lactate cytosol rather than being oxidized inside mitochondria. Thus, MPC activity expression might play key role fate during HF. The purpose this work was study levels two subunits carrier, named MPC1 MPC2, human hearts with HF different etiologies. Methods Protein mRNA...
Background Since mitochondria are the principal source of reactive oxygen species (ROS), these organelles may play an important role in ischemic cardiomyopathy (IC) development. The mitochondrial genome influence this disease. aim present study was to test relationship between IC development and impact single nucleotide polymorphisms (SNPs) DNA (mtDNA) defining haplogroups a population study. Methodology findings Ten major European were identified by using base extension technique polymerase...
Background. Nonhuman primates are potential permissive animals for studying the risk of in vivo infection with porcine endogenous retrovirus (PERV). Anti-αGal natural antibodies considered one barriers preventing PERV infection, and it has been postulated that reduction these could increase this infection. The aim study was to investigate role GAS 914, which depletes anti-αGal antibodies, transfer after pig-to-baboon organ xenotransplantation. Methods. Twenty-seven baboons underwent...
By using cytolytic T lymphocytes (CTL), the HLA-A2 serologic specificity may be divided into at least four subtypes designated as A2.1 to A2.4. The HLA-A2.4 antigen expressed by donor CLA is not recognized allogeneic CTL specific for either A2.1, A2.2, or A2.3, but indistinguishable from HLA-A2.1 H-Y-specific, HLA-A2-restricted and isoelectric focusing. structure of this was compared with known main subtype on JY cells establish molecular basis immunologic differences between two antigens....
The endogenous mechanisms contributing to tissue survival following myocardial infarction are not fully understood. We investigated the alterations in mitochondrial proteome after ischemia-reperfusion (I/R) and its possible implications on cell survival. Mitochondrial proteomic analysis of cardiac from an vivo porcine I/R model found that surviving peri-infarct border zone showed increased expression several proteins. Notably, these included subunits pyruvate carrier (MPC), namely MPC1 MPC2....