A5065549677- Neuroendocrine Tumor Research Advances
- Lung Cancer Research Studies
- Neuroblastoma Research and Treatments
- Pancreatic and Hepatic Oncology Research
- Helicobacter pylori-related gastroenterology studies
- Radiopharmaceutical Chemistry and Applications
- Prostate Cancer Treatment and Research
- Inflammatory Bowel Disease
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cardiac Fibrosis and Remodeling
- Cardiomyopathy and Myosin Studies
- Bioinformatics and Genomic Networks
- Microscopic Colitis
- Machine Learning in Healthcare
- Biochemical Analysis and Sensing Techniques
- Molecular Biology Techniques and Applications
- Cancer, Lipids, and Metabolism
- Digestive system and related health
- Gestational Trophoblastic Disease Studies
- Gene expression and cancer classification
- Gene Regulatory Network Analysis
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
Wren Laboratories (United States)
2014-2024
Queen Elizabeth University Hospital
2023
Imperial College London
2011-2020
Yale University
2008-2020
Medical University of Silesia
2020
IRCCS Ospedale San Raffaele
2020
King's College London
2009-2016
British Heart Foundation
2010-2015
University College London
2012-2015
Maastricht University
2015
Rationale: MicroRNAs (miRNAs) have been implicated in the epigenetic regulation of key metabolic, inflammatory, and antiangiogenic pathways type 2 diabetes (DM) may contribute to common disease complications. Objective: In this study, we explore plasma miRNA profiles patients with DM. Methods Results: Total RNA was extracted from samples prospective population-based Bruneck study. A total 13 candidate miRNAs identified by microarray screening network inference were quantified quantitative...
The National Cancer Institute's Surveillance, Epidemiology, and End Results (SEER) program has proven to be a significant resource in US neuroendocrine tumor (NET) epidemiology. Norway also holds robust detailed cancer registry: the Norwegian Registry of (NRC).SEER NET data were compared with corresponding NRC time period 1993 2004 determine whether there are differences epidemiology between United States.The SEER reported 17,312 2030 NETs, respectively. overall Caucasian incidence was 4.44,...
Rationale: Matrix vesicles (MVs), secreted by vascular smooth muscle cells (VSMCs), form the first nidus for mineralization and fetuin-A, a potent circulating inhibitor of calcification, is specifically loaded into MVs. However, processes fetuin-A intracellular trafficking MV biogenesis are poorly understood. Objective: The objective this study to investigate regulation, role, in VSMC calcification. Methods Results: Alexa488-labeled was internalized human VSMCs, trafficked via endosomal...
The differentiation of IL-10-producing regulatory B cells (Bregs) in response to gut-microbiota-derived signals supports the maintenance tolerance. However, whether microbiota-derived metabolites can modulate Breg suppressive function remains unknown. Here, we demonstrate that rheumatoid arthritis (RA) patients and arthritic mice have a reduction microbial-derived short-chain fatty acids (SCFAs) compared healthy controls mice, supplementation with SCFA butyrate reduces severity. Butyrate...
MicroRNA (miRNA) biomarkers are attracting considerable interest. Effects of medication, however, have not been investigated thus far.
Vascular calcification is prevalent in the aging population, yet little known of mechanisms driving age-associated vascular smooth muscle cell (VSMC) phenotypic change.To investigate role nuclear lamina disruption, a specific hallmark VSMC aging, osteogenic differentiation.Prelamin A, unprocessed form protein lamin accumulated calcifying human VSMCs vitro and vivo, its overexpression promoted differentiation mineralization. During vitro, prelamin A accumulation occurred concomitantly with...
Background— After myocardial ischemia, extracellular matrix (ECM) deposition occurs at the site of focal injury and border region. Methods Results— We have applied a novel proteomic method for analysis ECM in cardiovascular tissues to porcine model ischemia/reperfusion injury. proteins were sequentially extracted identified by liquid chromatography tandem mass spectrometry. For first time, such as cartilage intermediate layer protein 1, matrilin-4, adipocyte enhancer binding collagen...
Mechanisms by which gut luminal content regulates secretion and motility are ill understood. We evaluated whether neuroendocrine enterochromaffin (EC) cells act as sensors for a wide variety of nutrients defined the secretory mechanisms this process. Pure (98-99%) FACS-sorted human EC neoplastic (KRJ-I) were studied. RT-PCR identified transcripts T2R1 (bitter), OR1G1 (class II olfactory) trace amine (TAR1) G protein-coupled receptors (GPCRs) transporters glutamine (SNAT1/2), glucose...
Background— We sought to perform a systematic lipid analysis of atherosclerotic plaques using emerging mass spectrometry techniques. Methods and Results— A chip-based robotic nanoelectrospray platform interfaced triple quadrupole spectrometer was adapted analyze lipids in tissue sections extracts from human endarterectomy specimens by shotgun lipidomics. Eighteen scans for different classes plus additional fatty acids resulted the detection 150 species 9 which 24 were detected...
Rationale: MicroRNAs (miRNAs), in particular miR-29b and miR-30c, have been implicated as important regulators of cardiac fibrosis. Objective: To perform a proteomics comparison miRNA effects on extracellular matrix secretion by fibroblasts. Methods Results: Mouse fibroblasts were transfected with pre-/anti-miR their conditioned medium was analyzed mass spectrometry. targeted cadre proteins involved fibrosis, including multiple collagens, metalloproteinases, leukemia inhibitory factor,...
Gastroenteropancreatic (GEP) neuroendocrine neoplasms (NENs) are increasing in both incidence and prevalence. A delay correct diagnosis is common for these lesions. This reflects the absence of specific blood biomarkers to detect NENs. Measurement secretory peptide Chromogranin (CgA) used, but a single value, non-specific assay data highly variable. To facilitate tumor detection, we developed multi-transcript molecular signature PCR-based analysis. NEN transcripts were identified by...
Regulatory B cells (Bregs) play a critical role in the control of autoimmunity and inflammation. IL-10 production is hallmark for identification Bregs. However, molecular determinants that regulate transcription Breg developmental program remain unknown. Here, we demonstrate aryl hydrocarbon receptor (AhR) regulates differentiation function IL-10-producing CD19+CD21hiCD24hiBregs limits their into contribute to Chromatin profiling transcriptome analyses show loss AhR reduces expression by...
A key issue in gastroenteropancreatic neuroendocrine tumors (GEP-NETs) is early identification and prediction of disease progression. Clinical evaluation imaging are limited due to the lack sensitivity indolence. We assessed NETest as a predictive prognostic marker progression long-term follow-up study.GEP-NETs (n = 34) followed for median 4 years (2.2-5.4) were evaluated. WHO tumor grade/stage grade 1: n 17, 2: 14, 3: 1 (for 2, no was available); 31 (91%) stage IV. Baseline longitudinal...
A critical requirement in neuroendocrine tumor (NET) management is a sensitive, specific and reproducible blood biomarker test. We evaluated PCR-based 51 transcript signature (NETest) compared it to chromogranin (CgA), pancreastatin (PST) neurokinin (NKA). The multigene was two groups: i) validation set of 40 NETs controls ii) prospectively collected group (n=41, 61% small intestinal, 50% metastatic, 44% currently treated 41 age-sex matched controls). Samples were analyzed by two-step PCR...
The complexity of the clinical management neuroendocrine neoplasia (NEN) is exacerbated by limitations in imaging modalities and a paucity clinically useful biomarkers. Limitations currently available reflect difficulties measuring an intrinsically indolent disease, resolution inadequacies inter-/intra-facility device variability that RECIST (Response Evaluation Criteria Solid Tumors) criteria are not optimal for NEN. used biomarkers they secretory (chromogranin A, serotonin, neuron-specific...
A multianalyte algorithmic assay (MAAA) identifies circulating neuroendocrine tumor (NET) transcripts ( n =51) with a sensitivity/specificity of 98%/97%. We evaluated whether blood measurements correlated tissue transcript analysis. The latter were segregated into gene clusters (GC) that defined clinical ‘hallmarks’ neoplasia. MAAA/cluster integrated algorithm (CIA) was developed as predictive activity index to define behavior and outcome. three groups. Group 1: publically available NET...