A5024563468- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Autoimmune and Inflammatory Disorders Research
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Sex and Gender in Healthcare
- Gut microbiota and health
- Inflammatory Myopathies and Dermatomyositis
- COVID-19 Clinical Research Studies
- Cholesterol and Lipid Metabolism
- Diabetes and associated disorders
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Clostridium difficile and Clostridium perfringens research
- Cell Adhesion Molecules Research
- Steroid Chemistry and Biochemistry
- IL-33, ST2, and ILC Pathways
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 and COVID-19 Research
- Eosinophilic Esophagitis
- Adipokines, Inflammation, and Metabolic Diseases
- Spondyloarthritis Studies and Treatments
- COVID-19 and Mental Health
- Complement system in diseases
- Inflammasome and immune disorders
University College London
2016-2025
Versus Arthritis
2019-2025
Center for Rheumatology
2022-2025
Great Ormond Street Hospital
2012-2024
University of London
2018-2024
Ross School
2024
British Society for Rheumatology
2019-2021
The Francis Crick Institute
2020
University of Cape Town
2020
The London College
2020
Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected humoral immunity. spike glycoprotein (S)-reactive were detectable using a flow cytometry-based method SARS-CoV-2-uninfected individuals and particularly prevalent children adolescents. They predominantly the immunoglobulin G (IgG) class targeted S2 subunit. By contrast, infection induced higher titers S-reactive IgG...
Abstract IL-10–producing B cells, also known as regulatory cells (Bregs), play a key role in controlling autoimmunity. In this study, we report that chimeric mice specifically lacking (IL-10−/−B cell) developed an exacerbated arthritis compared with wild-type (WT) cell mice. A significant decrease the absolute numbers of Foxp3 T (Tregs), their expression level Foxp3, and marked increase inflammatory Th1 Th17 were detected IL-10−/− WT Reconstitution arthritic deficient (μMT) different subsets...
The differentiation of IL-10-producing regulatory B cells (Bregs) in response to gut-microbiota-derived signals supports the maintenance tolerance. However, whether microbiota-derived metabolites can modulate Breg suppressive function remains unknown. Here, we demonstrate that rheumatoid arthritis (RA) patients and arthritic mice have a reduction microbial-derived short-chain fatty acids (SCFAs) compared healthy controls mice, supplementation with SCFA butyrate reduces severity. Butyrate...
The inflammatory cytokine TNF-α has been recognized as a critical tumor promoter, but the effector cells that mediate its action have not fully characterized. Because B regulate squamous and prostate carcinogenesis, Tnf −/− mice harbor B-cell defects, we investigated hypothesis are important for TNF-α–mediated promotion of cancer development. Using an adoptive transfer strategy 7,12-dimethylbenz[α]anthracene/terephthalic acid (DMBA/TPA) two-stage model skin found both development...
Interleukin-10 (IL-10) producing B cells, also known as regulatory (Breg) play a key role in controlling autoimmunity. Our laboratory and others have demonstrated pivotal for Bregs rheumatological disorders, including experimental models of arthritis lupus. The aim this study was to identify the endogenous IL-10 secreting cells vivo induction disease progression collagen-induced (CIA).We generated chimeric mice that had knocked-out specifically cell population. These were compared with...
Regulatory B cells (Bregs) play a critical role in the control of autoimmunity and inflammation. IL-10 production is hallmark for identification Bregs. However, molecular determinants that regulate transcription Breg developmental program remain unknown. Here, we demonstrate aryl hydrocarbon receptor (AhR) regulates differentiation function IL-10-producing CD19+CD21hiCD24hiBregs limits their into contribute to Chromatin profiling transcriptome analyses show loss AhR reduces expression by...
Abstract Several related human coronaviruses (HCoVs) are endemic in the population, causing mild respiratory infections 1 . Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), etiologic agent of disease 2019 (COVID-19), is a recent zoonotic infection that has quickly reached pandemic proportions 2,3 Zoonotic introduction novel thought to occur absence pre-existing immunity target population. Using diverse assays for detection antibodies reactive with SARS-CoV-2 spike (S)...
Sex differences in immunity are well-documented, though mechanisms underpinning these remain ill-defined. Here, a human-only ex vivo study, we demonstrate that postpubertal cisgender females have higher levels of CD19+CD27+IgD− class-switched memory B cells compared with age-matched males. This increase is only observed after puberty and before menopause, suggesting strong influence for sex hormones. Accordingly, express high estrogen receptor 2 (ESR2), class-switch–regulating genes enriched...
Juvenile dermatomyositis (JDM) is a rare form of childhood autoimmune myositis that presents with proximal muscle weakness and skin rash. B cells are strongly implicated in the pathogenesis disease, but underlying mechanisms unknown. Therefore, main objective our study was to investigate driving cell lymphocytosis define pathological features Dermatomyositis patients. Patients were recruited through UK JDM Cohort Biomarker study. Peripheral blood subpopulations immunophenotyped by flow...
Regulatory B cells (Breg) express high levels of CD1d that presents lipid antigens to invariant natural killer T (iNKT) cells. The function in Breg biology and iNKT cell activity during inflammation remains unclear. Here we show, using chimeric mice, depletion adoptive transfer, CD1d-lipid presentation by Bregs induces secrete interferon (IFN)-γ contribute, partially, the downregulation helper (Th)1 Th17-adaptive immune responses ameliorate experimental arthritis. Mice lacking...
Summary Whilst innate B1-B cells are atheroprotective, adaptive B2-B considered pro-atherogenic. Different subsets of B regulatory (Breg) have been described. In experimental arthritis and lupus-like disease, Breg contained within the CD21hiCD23hiCD24hi cell pool. The existence role in vascular disease is not known. We sought to investigate existence, identity location disease. representation spleens lymph nodes (LNs) Apolipoprotein E-/- (ApoE-/-) mice compared controls was characterised by...
Obesity increases the risk of type 2 diabetes mellitus, cardiovascular disease, fatty liver and cancer. It is also linked with more severe complications from infections, including COVID-19, poor vaccine responses. Chronic, low-grade inflammation associated immune perturbations play an important role in determining morbidity people living obesity. The contribution B cells to dysregulation meta-inflammation obesity has been documented by studies over past decade. With a focus on human studies,...
Nicolaides-Baraitser syndrome (NBS) is an infrequently described condition, thus far reported in five cases. In order to delineate the phenotype and its natural history more detail, we gathered data on 18 hitherto unreported patients through a multi-center collaborative study, follow-up of earlier patients. A detailed comparison 23 provided. NBS distinct recognizable entity, probably has been underdiagnosed until now. Main clinical features are severe mental retardation with absent or...
To define the host mechanisms contributing to pathological interferon (IFN) type 1 signature in Juvenile dermatomyositis (JDM).