A5103101759- Hepatitis B Virus Studies
- Hepatitis C virus research
- Cardiac Fibrosis and Remodeling
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Topological Materials and Phenomena
- Cardiac Imaging and Diagnostics
- Advanced Condensed Matter Physics
- Liver Disease Diagnosis and Treatment
- High-pressure geophysics and materials
- Animal Virus Infections Studies
- Nitric Oxide and Endothelin Effects
- Cancer-related molecular mechanisms research
- Elasticity and Material Modeling
- Lymphoma Diagnosis and Treatment
- Magnetic properties of thin films
- Mesenchymal stem cell research
- Signaling Pathways in Disease
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Acute Myocardial Infarction Research
- Digestive system and related health
- Immune Cell Function and Interaction
- Lipid Membrane Structure and Behavior
Monash University
2006-2025
Australian Regenerative Medicine Institute
2025
Czech Academy of Sciences, Institute of Physics
2024
Chinese Academy of Sciences
2024
Cornell University
2024
Institute of Physics
2024
University of Chinese Academy of Sciences
2024
People's Hospital of Bishan District
2023
King's College Hospital
2001-2018
Jinan Central Hospital
2011-2017
Cardiac failure occurs when the heart fails to adapt chronic stresses. Reactive oxygen species (ROS)-dependent signaling is implicated in cardiac stress responses, but role of different ROS sources remains unclear. Here we report that NADPH oxidase-4 (Nox4) facilitates adaptation stress. Unlike other Nox proteins, Nox4 activity regulated mainly by its expression level, which increases cardiomyocytes under stresses such as pressure overload or hypoxia. To investigate functional during...
Rationale: MicroRNAs (miRNAs), in particular miR-29b and miR-30c, have been implicated as important regulators of cardiac fibrosis. Objective: To perform a proteomics comparison miRNA effects on extracellular matrix secretion by fibroblasts. Methods Results: Mouse fibroblasts were transfected with pre-/anti-miR their conditioned medium was analyzed mass spectrometry. targeted cadre proteins involved fibrosis, including multiple collagens, metalloproteinases, leukemia inhibitory factor,...
In recent years, various types of stem cells have been characterized and their potential for cardiac regeneration has investigated. We previously described the isolation broadly multipotent from amniotic fluid, defined as fluid (AFS) cells. The aim this study was to investigate therapeutic human AFS (hAFS) in a model acute myocardial infarction. Wistar rats underwent 30 min ischemia by ligation left anterior descending coronary artery, followed administration hAFS 2 h reperfusion. Infarct...
Under the new normal of economic development, China's emphasis on corporate social responsibility continues to increase. Institutional investors are often seen as an important force that can improve governance. Examining influence shareholding sustainable growth firms aids in comprehending how enhance governance structures foster development. This article focuses Chinese A-share listed from 2010 2021 subjects study. The two-way fixed effects method is employed examine impact institutional...
The reactive oxygen species–generating enzyme NADPH oxidase 4 (Nox4) is up-regulated in the heart after myocardial infarction (MI). Mice with cardiomyocyte-targeted Nox4 overexpression (TG) displayed increased macrophages at baseline, skewing toward an M2 phenotype compared wild-type controls (WT). After MI, TG mice had a higher proportion of along survival, decreased cardiac remodeling, and better contractile function than mice. post-MI increase matrix metalloproteinase–2 activity was...
Background Well‐defined inflammation, proliferation, and maturation phases orchestrate the remodeling of injured myocardium after myocardial infarction ( MI ) by controlling formation new extracellular matrix. The matrix consists mainly collagen but also fractions elastin. It is thought that elastin responsible for maintaining elastic properties myocardium, thus reducing risk premature rupture. An elastin/tropoelastin–specific contrast agent (Gd‐ ESMA was used to image tropoelastin mature...
Glutathione is the major intracellular redox buffer in liver and critical for hepatic detoxification of xenobiotics other environmental toxins. Hepatic glutathione also a systemic store organs thus impacts on pathologies such as Alzheimer's disease, Sickle Cell Anaemia chronic diseases associated with aging. levels are determined part by availability cysteine, generated from homocysteine through transsulfuration pathway. The partitioning between remethylation pathways known to be subject...
Abstract Background Assistance with exit from endocytic vesicles is a key factor for non‐viral gene delivery, and particular challenge in vivo . We have evaluated the use of chloroquine administered systemically, orally and/or locally delivery to liver. Methods The DNA vector (polylysine‐molossin) 31 amino acid bifunctional synthetic peptide, incorporating an terminal chain 16 lysines electrostatic binding DNA. Gene was right lateral lobes liver by branches bile duct or portal vein. Results...
p7 protein is a small encoded by Hepatitis C virus (HCV) that functions as an ion channel in planar lipid bilayers. The function of vital for the life cycle. In this study, genotype 2a (strain JFH1; only strain replicates and produces progeny vitro) was tagged with either enhanced green fluorescent (eGFP) or haemagglutinin (HA) epitope to facilitate tracking intracellular environment. viral polyprotein expressed transiently cells after transfection recombinant RNA transcripts. Confocal...
Abstract Background The major barrier to the clinical application of hydrodynamic gene delivery liver is large volume fluid required using standard protocols. Regional via branches portal vein has not previously been reported, and we have evaluated this approach in a rat model. Methods pGL3 plasmid with luciferase reporter was used at 50 µg/ml isotonic solutions, administered syringe pump for precise control conditions evaluated. Gene expression individually measured six anatomically...
The objective of this study was to evaluate a bifunctional synthetic peptide as DNA vector for regional gene delivery the rat liver by portal vein and bile duct routes. 31-amino-acid (polylysine-molossin) comprises an amino-terminal chain 16 lysines electrostatic binding DNA, 15 amino acid integrin-binding domain venom American pit viper, Crotalus molossus molossus. Initial in vitro evaluation demonstrated that polylysine-molossin/DNA complexes were much smaller (~50-100nm versus...
GB virus B (GBV-B) is a hepatotropic that closely related to hepatitis C (HCV). GBV-B causes acute in infected marmosets and tamarins therefore useful small-animal model for the study of HCV. We investigated virus-specific T-cell responses with GBV-B. Gamma interferon (IFN-gamma) enzyme-linked immunospot (ELISPOT) assay peripheral blood two were assessed throughout course infection. These directed against nonstructural proteins 3 (NS3), 4A (NS4A), 5B (NS5B), their appearance was temporally...
Two GB virus B (GBV-B) chimeric genomes, GBV-HVR and GBV-HVRh (with a hinge), containing the coding region of immunodominant hypervariable 1 (HVR1) E2 envelope protein Hepatitis C (HCV) were constructed. Immunoblot analysis confirmed that HVR1 was anchored to GBV-B protein. To investigate replication competence in vivo stability vitro -generated RNA transcripts, two naïve marmosets inoculated intrahepatically with transcripts. The genome detectable for 2 weeks post-inoculation (p.i.),...
Myocardial infarction (MI) results in adverse cardiac remodeling leading to heart failure and increased mortality. Experimental mouse models of MI are extensively used identify mechanisms underlying remodeling, but the extent that occurs may be highly variable can limit utility discover new disease pathways. The ability predict development significant late post-MI would invaluable conducting such studies by increasing throughput efficiency. This study aimed potential thresholds magnetic...
Background and aimsAcute ischemia is associated with myocardial endothelial damage microvessel formation, resulting in leakage of plasma albumin into the extravascular space. In this study, we tested whether an albumin-binding intravascular contrast agent (gadofosveset) allows for improved quantification permeability compared to conventional extracellular Gd-DTPA using late gadolinium enhancement (LGE) T1 mapping vivo.MethodsMI was induced C57BL/6 mice (n = 6) cardiac magnetic resonance...
Purpose To investigate a very small iron‐oxide particle (VSOP) in mouse model of acute ischemia‐reperfusion to access the mechanism such particles areas myocardial inflammation. Materials and Methods Animals were injected with VSOP at several time points, infarction (MI), before after MI. MRI was used localize enhancement, evaluate extension, determine related T2* values. Histology, electron microscopy, macrophage counting, Evan's Blue staining also performed. Results We found that uptake...
BackgroundBone marrow transplantation (BMT) is commonly used in experimental studies to investigate the contribution of BM-derived circulating cells different disease processes. During investigating cardiac response acute myocardial infarction (MI) induced by permanent coronary ligation mice that had previously undergone BMT, we found BMT itself affects remodelling response.Methods and resultsCompared matched naive mice, animals developed significantly less post-MI adverse remodelling,...
Intestinal lactase has potential as an autologous β-galactosidase reporter gene for long-term expression studies in vivo, using chromogenic, luminescent, and fluorogenic substrates developed Escherichia coli β-galactosidase. In normal rat tissues, reactivity with a chromogenic fucopyranoside (X-Fuc, the preferred substrate of lactase) was present only at lumenal surface small intestine epithelial cells. Full-length (domains I–IV), mature III IV), cytosolic form IV, without signal sequence or...