A5086044688- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Animal Virus Infections Studies
- Hepatitis Viruses Studies and Epidemiology
- Cervical Cancer and HPV Research
- Veterinary medicine and infectious diseases
- Liver Disease Diagnosis and Treatment
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Molecular Biology Techniques and Applications
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Biochemical and Molecular Research
- Liver Disease and Transplantation
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Cancer and Skin Lesions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal Health and Biochemistry
- Genital Health and Disease
- Endoplasmic Reticulum Stress and Disease
- Animal Disease Management and Epidemiology
- Global Cancer Incidence and Screening
- Research on Leishmaniasis Studies
Monash University
2007-2024
The University of Melbourne
2022-2024
Royal Women's Hospital
2022-2024
Murdoch Children's Research Institute
2022-2024
Australian Regenerative Medicine Institute
2017-2019
University of Qom
2014
National Institute of Genetic Engineering and Biotechnology
2013
Burnet Institute
2004-2012
Virginia–Maryland College of Veterinary Medicine
2001-2005
Virginia Tech
2001-2005
Hepatitis E virus (HEV) is an important public health concern in many developing countries. HEV also endemic some industrialized counties, including the United States. With our recent discovery of swine pigs that genetically closely related to human HEV, hepatitis now considered a zoonotic disease. Human strains are heterogenic. So far States, only one strain has been identified and characterized from pig. To determine extent genetic variations nature infections U.S. pigs, we developed...
Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens North America; the cause of this unknown. In study, genetic identification and characterization a novel virus related to human hepatitis E (HEV) isolated from bile samples with HS reported. Based upon similar genomic organization significant sequence identity HEV, has been tentatively named avian HEV order distinguish it swine HEV. Electron microscopy revealed that non-enveloped particle 30–35 nm diameter. The 3′ half...
ABSTRACT Hepatitis E virus (HEV) is the major cause of enterically transmitted non-A, non-B hepatitis in many developing countries and also endemic industrialized countries. Due to lack an effective cell culture system a practical animal model, mechanisms HEV pathogenesis replication are poorly understood. Our recent identification swine from pigs affords us opportunity systematically study model. In early study, we experimentally infected specific-pathogen-free with two strains HEV: US-2...
ABSTRACT Infection of animals with a molecular viral clone is critical to study the genetic determinants replication and virulence in host. Type 2 porcine circovirus (PCV2) has been incriminated as cause postweaning multisystemic wasting syndrome (PMWS), an emerging disease pigs. We report here for first time construction use infectious DNA PCV2 characterize pathologic lesions associated infection by direct vivo transfection pigs clone. The was generated ligating two copies complete genome...
We recently identified and characterized a novel virus, designated avian hepatitis E virus (avian HEV), from chickens with hepatitis-splenomegaly syndrome (HS syndrome) in the United States. Avian HEV is genetically related to but distinct human swine HEVs. To determine extent of genetic variation seroprevalence infection chicken flocks, we 11 additional isolates HS assessed prevalence antibodies total 1,276 different ages breeds 76 flocks five states (California, Colorado, Connecticut,...
We recently identified a novel virus, designated avian hepatitis E virus (avian HEV), from chickens with hepatitis–splenomegaly (HS) syndrome in the USA. showed that HEV is genetically related to swine and human HEVs. Here we report antigenic cross-reactivity of putative open reading frame 2 (ORF2) capsid protein those HEVs Australian chicken big liver spleen disease (BLSV). The region encoding C-terminal 268 amino acid residues ORF2 was cloned into expression vector pRSET-C. truncated...
Highlights•Antibody microarray to investigate mosquito host cell signaling response Wolbachia•Wolbachia-infected cells show downregulation of the insulin receptor (IR)•Inhibition or silencing IR impairs replication dengue and Zika viruses in vitro•Mosquitoes fed inhibitor have impaired virus replicationSummaryWolbachia-infected mosquitoes are refractory super-infection with arthropod-borne pathogens, but role proteins pathogen-blocking mechanisms remains be elucidated. Here, we use an...
ABSTRACT Swine hepatitis E virus (swine HEV), the first animal strain of HEV to be isolated, is a zoonotic agent. We report here construction and in vitro vivo characterizations infectious cDNA clones swine HEV. Eight overlapping fragments spanning entire genome were amplified by reverse transcription-PCR assembled into full-length clone, clone C, which contained 14 mutations compared consensus sequence RNA transcripts from C not infectious, as determined intrahepatic inoculation pigs...
Hepatitis C virus (HCV) causes chronic hepatitis in 2-3% of world population and remains one the health threatening human viruses, worldwide. In absence an effective vaccine, therapeutic approach is only option to combat C. Interferon-alpha (IFN-alpha) ribavirin (RBV) combination alone or with recently introduced new direct-acting antivirals (DAA) used treat patients infected HCV. The present study utilized feature selection methods (Gini Index, Chi Squared machine learning algorithms) other...
Objective WHO recommends human papillomavirus (HPV) testing for cervical screening, with triage of high-risk HPV (hrHPV) positive women. However, there are limitations to effective low-resource, high-burden settings, such as Papua New Guinea. In this exploratory study, we assessed the performance host methylation tools predicting high-grade squamous intraepithelial lesions (HSIL) in self-collected and clinician-collected samples. Design Exploratory observational study. Setting Provincial...
ABSTRACT The p7 protein of hepatitis C virus (HCV) is a viroporin that dispensable for viral genome replication but plays critical role in morphogenesis. In this study, we generated JFH1-based intergenotypic chimeric encoded heterologous genotype 1b (GT1b) p7. parental was nonviable human hepatoma cells, and infectious virions were produced only when cells transfected with the genomes passaged several times. Sequence analysis entire polyprotein-coding region recovered revealed one...
p7 protein is a small encoded by Hepatitis C virus (HCV) that functions as an ion channel in planar lipid bilayers. The function of vital for the life cycle. In this study, genotype 2a (strain JFH1; only strain replicates and produces progeny vitro) was tagged with either enhanced green fluorescent (eGFP) or haemagglutinin (HA) epitope to facilitate tracking intracellular environment. viral polyprotein expressed transiently cells after transfection recombinant RNA transcripts. Confocal...
GB virus B (GBV-B) is a hepatotropic that closely related to hepatitis C (HCV). GBV-B causes acute in infected marmosets and tamarins therefore useful small-animal model for the study of HCV. We investigated virus-specific T-cell responses with GBV-B. Gamma interferon (IFN-gamma) enzyme-linked immunospot (ELISPOT) assay peripheral blood two were assessed throughout course infection. These directed against nonstructural proteins 3 (NS3), 4A (NS4A), 5B (NS5B), their appearance was temporally...
Summary. The p7 protein of hepatitis C virus (HCV) has been classified into a family viral proteins, designated viroporins that form ion channels. M2 influenza is the prototype viroporin and encodes HXXXW motif constitutes main functional element Alignment different proteins revealed sequence (positions 17–21) also highly conserved among some HCV genotypes. To study putative in p7, five mutants Japanese fulminant 1 strain encoded H17A, H17G, H17E, Y21A Y21W were generated. After transfection...
DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 34:177-185 (1998) - doi:10.3354/dao034177 Putative spawner-isolated mortality virus associated with mid-crop syndrome in farmed Penaeus monodon from northern Australia Leigh Owens1,*, Gholamreza Haqshenas, Catriona McElnea, Robert Coelen Department Microbiology and Immunology, Australian Institute Tropical Veterinary...
Two GB virus B (GBV-B) chimeric genomes, GBV-HVR and GBV-HVRh (with a hinge), containing the coding region of immunodominant hypervariable 1 (HVR1) E2 envelope protein Hepatitis C (HCV) were constructed. Immunoblot analysis confirmed that HVR1 was anchored to GBV-B protein. To investigate replication competence in vivo stability vitro -generated RNA transcripts, two naïve marmosets inoculated intrahepatically with transcripts. The genome detectable for 2 weeks post-inoculation (p.i.),...
Abstract Host cell signalling during infection with intracellular pathogens remains poorly understood. Here we report on the use of antibody microarray technology to detect variations in expression levels and phosphorylation status host proteins hepatitis C virus (HCV) replication. Following transfection HCV RNA, JNK NF-κB pathways are suppressed, while JAK/STAT5 pathway is activated; furthermore, components apoptosis cycle control machineries affected and/or status. RNAi-based hit...
Summary The p7 protein of hepatitis C virus ( HCV ) is a small, integral membrane that plays critical role in replication. Recently, we reported two intergenotypic JFH 1 chimeric viruses encoding the partial or full‐length ‐A strain genotype 1b GT 1b; Virology ; 2007; 360:134). In this study, determined consensus sequences entire polyprotein coding regions wild‐type and revertant identified predominant amino acid substitutions core K 74 M ), NS 2 T 23 N , H 99 P 5A D 251 G ). Forward genetic...
Abstract The efficiency of PCR-based diagnostic assays can be impacted by the quality DNA template, and anal samples particularly problematic due to presence faecal contaminants. Here, we compared Quick-DNA Viral Kit (Zymo, Zymo Research, CA) MagNA Pure 96 NA Small Volume (MP96, Roche) for use Seegene Anyplex II HPV28 assay (Anyplex28, Seegene) with samples. A total 94 extracted using MP96 kits were tested via Anyplex28, which detects high-risk human papillomavirus (HR-HPV, Panel A) low-risk...
The dengue virus membrane (M) protein is a key component of the mature virion. Here, we characterised cellular behaviour M using recombinant construct to understand its inherent properties. Using confocal microscopy, showed that and intracellular precursor, prM, localised endoplasmic reticulum. was also detected on cell surface secreted, suggesting can enter secretory pathway. In addition, cross‐linking studies form dimers tetramers. These findings suggest behaves as analogous major envelope E.