A5075904496- Cervical Cancer and HPV Research
- Genital Health and Disease
- Hepatitis B Virus Studies
- Colorectal and Anal Carcinomas
- Reproductive tract infections research
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Viral-associated cancers and disorders
- Epigenetics and DNA Methylation
- Endometrial and Cervical Cancer Treatments
- Global Cancer Incidence and Screening
- RNA modifications and cancer
- Urinary Tract Infections Management
- Microbial infections and disease research
- Literacy and Educational Practices
- Ethics and bioethics in healthcare
- Urban Development and Societal Issues
- Educational methodologies and cognitive development
- Reconstructive Surgery and Microvascular Techniques
- Logistics and Infrastructure Analysis
- Sympathectomy and Hyperhidrosis Treatments
- Genetic factors in colorectal cancer
- T-cell and Retrovirus Studies
- Medical research and treatments
- Child Abuse and Related Trauma
Royal Women's Hospital
2015-2024
Murdoch Children's Research Institute
2020-2024
Instituto Nacional de Cancerología
2005-2021
Royal Ottawa Mental Health Centre
2021
Universidad Nacional de Colombia
2010
National Cancer Institute
2008
Vrije Universiteit Amsterdam
2002-2005
An inverse relationship between age and human papillomavirus (HPV) prevalence has been reported in many developed countries, but information on this is scarce other parts of the world. We carried out a cross-sectional study sexually active women from general population 15 areas 4 continents. Similar standardised protocols for women's enrolment, cervical specimen collection PCR-based assays HPV testing were used. different groups was compared by area. 18,498 aged 15-74 years included....
Data on the incidence and determinants of human papillomavirus (HPV) infection in women 130 years old are scarce. To address this, a cohort 1610 women—15–85 old, HPV negative, with normal cytological results at baseline—was monitored every 6 months for an average 4.1 years. Information risk factors cervical samples testing detection typing DNA were obtained each visit. The high-risk types was higher than that low-risk (5.0 vs. 2.0 cases/100 woman-years). agespecific curve bimodal, whereas...
Little is known about the factors that influence clearance of human papillomavirus (HPV), primary cause cervical carcinoma. A total 227 women cytologically normal and HPV positive at baseline were identified from a population-based cohort 1,995 Bogota, Colombia, aged 13-85 years followed between 1993 2000 (mean follow-up, 5.3 years). DNA detection viral load determination based on GP5+/GP6+ polymerase chain reaction enzyme immunoassay. Rate ratio estimates for calculated by using methods...
Human papillomavirus is the principal risk factor associated with cervical cancer, most common malignancy among women in Colombia. We conducted a survey, aiming to report type specific prevalence and determinants of human infection normal cytology. A total 1859 from Bogota, Colombia were interviewed tested for using general primer GP5+/GP6+ mediated PCR-EIA. The overall HPV DNA was 14.8%; 9% infected by high types, 3.1% low 2.3% both risk/low types 0.4% uncharacterized (human X). Thirty-two...
The natural course ofChlamydia trachomatis infection and its risk factors were studied in Colombian women with normal cytological results, during a 5-year period.Eighty-two who found to be positive for C. at the start of study 6-month intervals.At each visit, cervical scrape sample was obtained detection by use endogenous-plasmid polymerase chain reaction (PCR)-enzyme immunoassay VD2-PCR-reverse line blot assay.Of studied, 67% had single-serovar infection, 10% mixed-serovar 23% an...
Abstract Human papillomavirus (HPV) is a sexually transmitted infection but it unclear whether differences in transmission efficacy exist between individual HPV types. Information on sexual behavior was collected from 11 areas four continents among population-based, age-stratified random samples of women ages ≥15 years. testing done using PCR-based enzyme immunoassay. Unconditional logistic regression used to estimate odds ratios (OR) being positive and corresponding 95% confidence intervals...
Coinfection with multiple types of human papillomavirus (HPV) and its implications for the development efficacious HPV vaccines is a subject great interest. To describe occurrence concurrent infection to determine whether genital modifies risk acquiring new another type, 1610 subjects were monitored an average 4.1 years in Bogota, Colombia. Information on factors cervical cells was collected detection DNA 36 at study entry 6 consecutive 6-month follow-up visits. Clustering or acquisition...
Little is known about the dynamics of human papillomavirus (HPV) infection and subsequent development high-grade cervical intraepithelial neoplasia (CIN2/3), particularly in women >30 years age. This information needed to assess impact HPV vaccines consider new screening strategies. A cohort 1728 15-85 old with normal cytology at baseline was followed every 6 months for an average 9 years. Women squamous lesions were referred biopsy treatment. The Kaplan-Meier method used estimate median...
The aim of the present study was to estimate prevalence Kaposi sarcoma-associated herpesvirus (KSHV) in female general population, define geographic variation and heterosexual transmission virus.The included 10,963 women from 9 countries for whom information on sociodemographic characteristics reproductive, sexual, smoking behaviors were available. Antibodies against KSHV that encoded lytic antigen K8.1 latent ORF73 determined.The range (defined as detection any antigen) 3.81%-46.02%, with...
Gay and bisexual men (GBM) are disproportionately affected by anal cancer. Prevention is hindered incomplete understanding of the natural history its precursor, high-grade squamous intraepithelial lesions (HSIL).The Study Anal Cancer, conducted between 2010 2018, enrolled human immunodeficiency virus (HIV)-positive HIV-negative GBM aged ≥35 years. cytology high-resolution anoscopy (HRA) were performed at baseline 3 annual visits. A composite HSIL diagnosis (cytology ± histology) was used....
Clinical parameters and proteins have recently been suggested as possible causes of radiotherapy (RT) resistance in cervical carcinoma (CC). The objective the present study was to validate prognostic biomarkers radiation resistance. prospective included patients undergoing RT with curative intent for histologically proven locally advanced squamous cell CC. Tissues blood samples were systematically collected before initiation. Immuno-histochemistry performed (IGF-IR α β, GAPDH, HIF-1 alpha,...
Abstract Human papillomavirus (HPV) is detected in up to 96% of anal squamous cell cancers, where screening programs needed. However, the best methodology still undetermined. Host DNA methylation markers CADM1, MAL and miR124 have been identified cervical disease, but not disease. Anal swabs varying by disease grade were assessed for miR124-2. Each marker was compared across grades, stratified HPV HIV status. Receiver operating characteristic curves predictive value significant gene...
In this study we developed and evaluated a new PCR-based typing assay, directed to the VD2 region of omp1 gene, for detection urogenital Chlamydia trachomatis infections. A nested PCR-reverse line blot (RLB) assay was nine different serovars C. trachomatis. The tested with reference strains cervical scrapes 86 Colombian women previously found be positive by using plasmid PCR. Two sets primers gene were designed, fragments 220 166 bp generated in primary PCRs, respectively. addition, an RLB...
<b>Objectives:</b><i>Chlamydia trachomatis</i> infection in the cervix and uterus has been hypothesised to be a co-factor for cervical cancer. We performed cross sectional study Bogota, Colombia, where cancer rates are high, determine prevalence determinants of <i>C infection, particular its association with human papillomavirus (HPV). <b>Methods:</b> 1829 low income sexually active women were interviewed tested trachomatis</i>, using an endogenous plasmid PCR-EIA, 37 HPV types, general...
: Some high-grade cervical lesions and cancers (HSIL+) test negative for human papillomavirus (HPV). The HPV-negative fraction varies between 0.03% 15% different laboratories. Monitoring extended re-analysis of HSIL+ could thus be helpful to monitor performance HPV testing services. We aimed a) provide a real-life example quality assurance (QA) program based on b) develop international guidance QA services standardized identification apparently re-analysis, either by the primary laboratory...
Australia introduced a national human papillomavirus (HPV) vaccination program for girls in 2007 and boys 2013, achieving high coverage. We assessed HPV prevalence among men who have sex with women (MSW) (MSM) aged 18-35 years examined effects. Between 2015-2018, self-collected penile or intra-anal swab genotyping. Vaccination status was confirmed the National Register. by age groups status. Prevalence of quadrivalent vaccine-targeted types (6, 11, 16, 18) 10.6% (95% confidence interval...
ABSTRACT We compared real-time LightCycler and TaqMan assays the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both PCR determined HPV16 specimens similarly. The level of agreement between these PCR/EIA was low ( P = 0.004), suggesting that latter method is not suited for quantifying DNA.
Human Papillomavirus (HPV) vaccines have been considered potentially cost-effective for the reduction of cervical cancer burden in developing countries; their effectiveness a public health setting continues to be researched. We conducted an HPV prevalence survey among Colombian women with invasive cancer. Paraffin-embedded biopsies were obtained from one high-risk and low-middle-risk regions. GP5+/GP6+ L1 primers, RLB assays, E7 type specific PCR used HPV-DNA detection. 217 cases analyzed...
Introduction Anal squamous cell carcinoma is preceded by persistent infection with high-risk human papillomavirus (HPV) and the cancer precursor, high-grade intraepithelial lesion (HSIL). Detection of specific HPV genotypes HPV-related biomarkers may be an option for primary anal screening. However, more data on natural history lesions are required. The outcomes from this study will enhance our understanding clinical biological behaviour inform development future genotype and/or biomarker...
Incidence and mortality rates of anal cancer are increasing globally. More than 90% squamous cell carcinomas (ASCC) associated with human papillomavirus (HPV). Studies on HPV-related anogenital lesions have shown that patterns methylation viral cellular DNA targets could potentially be developed as disease biomarkers. Lesion-specific isolated from formalin-fixed paraffin-embedded (FFPE) tissues existing or prospective patient cohorts may constitute a valuable resource for analysis. However,...
Low grade squamous intra-epithelial lesions could be considered as a manifestation of human papillomavirus exposition, however the discrepancy between rates infection with and development low is notable. Here we report cross-sectional three-armed case-control study in Colombian population, to compare risk factors women that DNA-negative positive normal cytology.
Background: There exists limited information on the role of hTERT methylation, and its association with type-specific HPV infections in cervical cancer. Materials Methods: Eighty-seven frozen samples were analyzed for infection using a GP5<sup>+</sup>/GP6<sup>+</sup> PCR-RLB assay (RLB). DNA methylation analysis was performed newly developed PCR-RLB-hTERT. Results: Ninety-three percent HPV-positive fifteen different types detected. region 1 revealed no 78.8% partial 21.2%. In two, 68.2%...