A5076666539- Prenatal Screening and Diagnostics
- Pregnancy and preeclampsia studies
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Fetal and Pediatric Neurological Disorders
- Gestational Diabetes Research and Management
- MicroRNA in disease regulation
- Parvovirus B19 Infection Studies
- RNA modifications and cancer
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Thyroid Disorders and Treatments
- Genomic variations and chromosomal abnormalities
- Cancer Immunotherapy and Biomarkers
- Metabolism and Genetic Disorders
- Cardiac Valve Diseases and Treatments
- Birth, Development, and Health
- Mesenchymal stem cell research
- Melanoma and MAPK Pathways
- Cancer-related molecular mechanisms research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CAR-T cell therapy research
- Mitochondrial Function and Pathology
Nanjing Medical University
2016-2025
First Affiliated Hospital of Zhengzhou University
2023-2025
Southern Medical University
2025
Nanfang Hospital
2025
Yuhuangding Hospital
2023-2025
Qingdao University
2023-2025
McGill University Health Centre
2014-2024
Nanjing Maternity and Child Health Care Hospital
2017-2024
Xinyang Normal University
2024
Guangxi Medical University
2024
Abstract Background Tumor-associated macrophages (TAMs) are alternatively activated cells induced by interleukin-4 (IL-4)-releasing CD4 + T cells. TAMs promote breast cancer invasion and metastasis; however, the mechanisms underlying these interactions between tumor that lead to metastasis remain elusive. Previous studies have found microRNAs (miRNAs) circulating in peripheral blood identified microvesicles, or exosomes, as mediators of cell-cell communication. Therefore, one alternative...
Cardiac failure occurs when the heart fails to adapt chronic stresses. Reactive oxygen species (ROS)-dependent signaling is implicated in cardiac stress responses, but role of different ROS sources remains unclear. Here we report that NADPH oxidase-4 (Nox4) facilitates adaptation stress. Unlike other Nox proteins, Nox4 activity regulated mainly by its expression level, which increases cardiomyocytes under stresses such as pressure overload or hypoxia. To investigate functional during...
Mesenchymal stem cells (MSCs) have been found to benefit patients with a variety of ischemic diseases via promoting angiogenesis. It is also well established that exosomes secreted from MSCs deliver bioactive molecules, including microRNAs (miRs) recipient cells. Therefore, we hypothesized miRs into endothelial and mediate The pro-angiogenic stimulatory capacity was investigated using tube-like structure formation spheroid-based sprouting human umbilical vein (HUVECs), in vivo Matrigel plug...
NADPH oxidase (Nox)-derived reactive oxygen species (ROS) are known to be involved in angiotensin II-induced hypertension and endothelial dysfunction. Several Nox isoforms expressed the vessel wall, among which Nox2 is especially abundant endothelium. Endothelial levels rise during but little about cell-specific role of vivo. To address this question, we generated transgenic mice with endothelial-specific overexpression (Tg) studied effects on function blood pressure. Tg had an twofold...
Introduction microRNAs (miRs), a novel class of small non-coding RNAs, are involved in cell proliferation, differentiation, development, and death. In this study, we found that miR-221 translocation by microvesicles (MVs) plays an important role cardioprotection mediated GATA-4 overexpressed mesenchymal stem cells (MSC). Methods Results Adult rat bone marrow MSC neonatal ventricle cardiomyocytes (CM) were harvested as primary cultures. transduced with (MSCGATA-4) using the murine virus...
Objective To explore the feasibility of high-throughput massively parallel genomic DNA sequencing technology for noninvasive prenatal detection fetal sex chromosome aneuploidies (SCAs). Methods The study enrolled pregnant women who were prepared to undergo testing (NIPT) in second trimester. Cell-free (cffDNA) was extracted from mother's peripheral venous blood and a procedure undertaken. Patients identified as having pregnancies associated with SCAs offered chromosomal karyotyping. Results...
Licochalcone A (LicA), a flavonoid isolated from the famous Chinese medicinal herb Glycyrrhiza uralensis Fisch, has wide spectrum of pharmacological activities. In this study, anti-cancer effects and potential mechanisms LicA in non-small cell lung cancer (NSCLC) cells were studied. decreased viability induced apoptosis dose-dependent manner NSCLC cells. inhibited growth by blocking cycle progression at G2/M transition inducing apoptosis. treatment expression MDM2, Cyclin B1, Cdc2 Cdc25C...
Exosomes, carriers to transfer endogenous molecules, derived from bone marrow-derived mesenchymal stem cells (BMSCs) have been reported play a role in the progression of bladder cancer. Here we aimed test functional mechanism microRNA-9-3p (miR-9-3p)-containing exosomes BMSCs were cocultured with cancer cells, and secreted identified. Next, expression miR-9-3p endothelial cell-specific molecule 1 (ESM1) tissues was determined. Then effects ESM1 via BMSC-derived on cell viability, migration,...
Gestational diabetes mellitus (GDM) is associated with an increased risk of adverse pregnancy outcomes. Increasing evidence shows that placentation defects may play important roles in GDM. However, our understanding the human placenta remains limited. In this study, we generated a comprehensive transcriptomic profile cellular signatures and transcriptomes GDM using single-cell RNA sequencing (scRNA-seq), constructed cell atlas, identified subtypes subtype-specific marker genes. addition,...
Trophoblasts, important functional cells in the placenta, play a critical role maintaining placental function.The heterogeneity of trophoblasts has been reported, but little is known about trophoblast subtypes and distinctive functions during preeclampsia (PE).In this study, we aimed to gain insight into cell type-specific transcriptomic changes by performing unbiased single-cell RNA sequencing (scRNA-seq) tissue samples, including those patients diagnosed with PE matched healthy controls.A...
Objective— The mechanisms underlying the pathogenesis of aortic valve calcification remain unclear. With accumulating evidence demonstrating that recapitulates bone development, crucial roles noncanonical Wnt ligands WNT5a, WNT5b, and WNT11 in osteogenesis make them critical targets study calcification. Approach Results— Using immunohistochemistry, real-time qPCR, Western blotting, tissue culture, we examined distribution noncalcified calcified valves their effects on human interstitial...
Lipoprotein(a), or Lp(a), significantly increased alkaline phosphatase activity, release of phosphate, calcium deposition, hydroxyapatite, cell apoptosis, matrix vesicle formation, and phosphorylation signal transduction proteins; expression chondro-osteogenic mediators; decreased SOX9 Gla protein (p < 0.001). Inhibition MAPK38 GSK3β reduced Lp(a)-induced calcification human aortic valve interstitial cells There was abundant presence Lp(a) E06 immunoreactivity in diseased valves. The present...
Rationale: Glioma is the most common malignant primary brain tumor in central nervous system (CNS).The lack of reliable noninvasive diagnostic and prognostic methods one main reasons for high mortality glioma.Serum has become a useful biomarker diagnosis prognosis prediction glioma because extracellular vesicles (EVs) carry molecular components from their parental cells.Methods: To detect EVs perform analysis serum EVs, we established optimized microbead-assisted method based on flow...
Forty-five pregnant women who underwent cesarean section, including 30 cases of gestational diabetes mellitus (GDM) and 15 normal women, were enrolled in this study to examine the differential expression circular RNAs (circRNAs) placentas with GDM by RNA sequencing (RNA-seq) analysis. The differentially expressed circRNAs analyzed bioinformatically using Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment circRNA-microRNA (miRNA) interaction prediction. Quantitative...
Preeclampsia (PE) is the main cause of maternal death in primipara, and commonly results severe neonatal complications such as multiple organ dysfunction syndrome. However, exact pathogenesis this disease remains unclear. Circular RNAs (circRNAs) are noncoding that have been shown to be extensively involved numerous physiological processes, but there limited knowledge their functions mechanisms PE. In present study, we found expression a circRNA, hsa_circ_0088227 (circRNA...
Rab25, an epithelial-specific member of the Rab family small guanosine triphosphatases, is associated with several human cancers. The goal this study was to determine its function in bladder cancer (BC). We examined Rab25 expression pattern two different cohorts BC patients treated radical cystectomy by quantitative PCR, western blotting and immunohistochemical staining. A series vitro vivo assays were performed elucidate underlying mechanisms. significantly elevated at both messenger RNA...
NADPH oxidase-4 (Nox4) is an important reactive oxygen species (ROS) source that upregulated in the haemodynamically overloaded heart. Our previous studies using global Nox4 knockout (Nox4KO) mice demonstrated a protective role of during chronic abdominal aortic banding, involving paracrine enhancement myocardial capillary density. However, other authors who studied cardiac-specific Nox4KO reported detrimental effects response to transverse constriction (TAC). It has been speculated these...
To review the characteristics of newborn screening congenital hypothyroidism (CH), we reviewed data, including levels blood spot thyroid-stimulating hormone (TSH), and serum TSH free thyroxine (FT4), all infants who accepted program during last 14 years. In total, 437,342 underwent CH 192 were diagnosed with incidence was 1:2278. The positive rate initial 0.96%, predictive value 4.8%. We also designed a target sequencing panel 13 causative genes: DUOX2, TG, TPO, TSHR, TTF1, TTF2, PAX8,...