A5005758743- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Tissue Engineering and Regenerative Medicine
- Cardiac Valve Diseases and Treatments
- Apelin-related biomedical research
- Cardiovascular Disease and Adiposity
- Lipoproteins and Cardiovascular Health
- Congenital heart defects research
- Cardiomyopathy and Myosin Studies
- Hormonal Regulation and Hypertension
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cardiac Fibrosis and Remodeling
- Adipose Tissue and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- Pulmonary Hypertension Research and Treatments
- Immune Cell Function and Interaction
- Infective Endocarditis Diagnosis and Management
- Mesenchymal stem cell research
- Mast cells and histamine
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cardiac Structural Anomalies and Repair
- Cardiovascular Function and Risk Factors
- Pluripotent Stem Cells Research
- Renin-Angiotensin System Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Cholesterol and Lipid Metabolism
McGill University Health Centre
2014-2025
McGill University
2013-2025
Montreal Children's Hospital
2025
Royal Victoria Hospital
2022
Royal Victoria Regional Health Centre
2022
Royal Ottawa Mental Health Centre
2022
University of Sharjah
2018
Montreal General Hospital
2014-2016
Montreal Heart Institute
2016
Université de Montréal
2016
Objective— The mechanisms underlying the pathogenesis of aortic valve calcification remain unclear. With accumulating evidence demonstrating that recapitulates bone development, crucial roles noncanonical Wnt ligands WNT5a, WNT5b, and WNT11 in osteogenesis make them critical targets study calcification. Approach Results— Using immunohistochemistry, real-time qPCR, Western blotting, tissue culture, we examined distribution noncalcified calcified valves their effects on human interstitial...
Lipoprotein(a), or Lp(a), significantly increased alkaline phosphatase activity, release of phosphate, calcium deposition, hydroxyapatite, cell apoptosis, matrix vesicle formation, and phosphorylation signal transduction proteins; expression chondro-osteogenic mediators; decreased SOX9 Gla protein (p < 0.001). Inhibition MAPK38 GSK3β reduced Lp(a)-induced calcification human aortic valve interstitial cells There was abundant presence Lp(a) E06 immunoreactivity in diseased valves. The present...
C ardiovascular diseases remain the primary cause of death. 1 Myocardial infarction (MI), angina, and stroke take >16 million lives every year, but underlying mechanisms by which these often-fatal cardiovascular events occur tend to escape attention. 2Atherosclerosis is pathological inflammatory vascular disease not only responsible for most MIs strokes, it accounts 29% deaths worldwide. 1,34][5] Atherosclerosis characterized accumulation lipids, fibrous elements, cells within wall medium...
Exercise is beneficial for a variety of age-related disorders. However, the molecular mechanisms mediating adaptations to exercise in older adults are not well understood. The aim current study was utilize dual approach characterize genetic and metabolic adaptive pathways altered by veteran athletes age-matched untrained individuals. Two groups 50–60 year old males: competitive cyclists (athletes, n = 9; VO2peak 59.1±5.2 ml·kg−1·min−1; peak aerobic power 383±39 W) untrained, minimally active...
IL-15 is an inflammatory cytokine secreted by many cell types. also produced during physical exercise skeletal muscle and has been reported to reduce weight gain in mice. Contrarily, our findings on knockout (KO) mice indicate that promotes obesity. The aim of this study investigate the mechanisms underlying pro-obesity role adipose tissues.Control KO were maintained high fat diet (HFD) or normal control diet. After 16 weeks, body weight, tissue mass, serum lipid levels gene/protein...
Urotensin II (UII) is a potent vasoactive peptide that binds to the urotensin receptor-coupled receptor-14 (known as UT) and exerts wide range of actions in humans experimental animals. We tested hypothesis UII gene deletion or UT blockade ameliorate atherosclerosis.We observed significant reduction weight gain, visceral fat, blood pressure, circulating plasma lipids, proatherogenic cytokines improvement glucose tolerance knockout mice compared with wild type (P<0.05). Deletion after an...
Background Deep vein thrombosis (DVT) is the primary cause of pulmonary embolism and third most life-threatening cardiovascular disease in North America. Post-DVT anticoagulants, such as warfarin, heparin, direct oral reduce incidence subsequent venous thrombi. However, all currently used anticoagulants affect bleeding time at various degrees, there therefore a need for improved therapeutic regimens DVT. It has recently been shown that mast cells play crucial role DVT murine model. The...
Aortic valve stenosis (AVS) is a prevailing and life-threatening cardiovascular disease in adults over 75 years of age. However, the molecular mechanisms governing pathogenesis AVS are yet to be fully unraveled. With accumulating evidence that Wnt signaling plays key role development AVS, involvement intracellular molecules has become an integral study target pathogenesis. Thus, we hypothesized Wnt/β-catenin pathway mediators, SFRP2, DVL2, GSK3β β-catenin dysregulated patients with AVS....
Introduction The progression of coronary atherosclerosis is an active and regulated process. Wnt signaling pathway thought to play role in the pathogenesis several cardiovascular diseases; however, a better understanding this system yet be unraveled. Methods In study, real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) Western blotting were used quantify expression Wnt3a, Wnt5a, Wnt5b human plaque, immunohistochemistry was identify sites local expression. To...
Urotensin II (UII) is a vasoactive peptide that was first discovered in the teleost fish, and later mammals humans. UII binds to G protein coupled receptor GPR14 (now known as UT). mediates important physiological pathological actions by interacting with its receptor. The metabolic syndrome (MetS) described cluster of factors such obesity, dyslipidemia, hypertension, insulin resistance (IR), further leading development type 2 diabetes mellitus cardiovascular diseases. levels are upregulated...
The biogenesis of high-density lipoprotein (HDL) particles by cholesterol-laden foam cells in atherosclerotic lesions is crucial for the removal excess cholesterol from lesions. Impairment HDL biogenic process contributes to progression atherosclerosis. aim this study identify novel cellular factors regulating biogenesis.HDL a apolipoprotein (apo)-mediated solubilization specific plasma membrane (PM) microdomains generated cholesterol-accumulated cells. We established new method isolate PM...
The Hippo-signaling pathway is a mechanism implicated in cardiomyocyte cytoprotection and regeneration after myocardial infarction. Yes-associated protein 1, the main effector of this pathway, acts as co-transcriptional activator to promote proliferation survival. However, biological mechanisms by which yes-associated 1 protects heart post-MI are currently unknown. Here, we propose that plays critical role simulated ischemia-reperfusion injury. AC16 human cardiomyocytes were infected with...
The metabolic syndrome is defined by the presence of hyperlipidemia, obesity, hypertension, and diabetes. associated with significant cardiovascular morbidity mortality. aim present study was to determine role vasoactive peptide urotensin II (UII) in pathogenesis syndrome. We used obese mice (ob/ob) effect UII receptor (UT) blockage on different facets special emphasis cardiac function. Our data demonstrate a increase UT expression myocardium accompanied decrease sarco/endoplasmic reticulum...
Acute myocardial infarction (MI) remains one of the leading causes death worldwide with no curative therapy available. Stem cell therapies have been gaining interest as a means to repair cardiac tissue after MI and prevent onset heart failure. Many in vivo reports suggest that use stem cells is promising, yet clinical trials fail integrate into native tissue, resulting limited improvements function repair. To battle this limitation, combination using embedded bioactive scaffold promotes...
Background and aims: The aims of the present study were to determine expression urotensin II (UII), urotensin-II related peptide (URP) their receptor (UT) in stable unstable carotid atherosclerosis, effects UII on human aortic smooth muscle cell (SMCs) calcification. Methods Results: We examined UII, URP UT protein 88 endarterectomy specimens using immunohistochemistry. Expression was more evident compared plaques (P<0.05). Multivariate Spearman correlation analyses revealed significant...