A5025712968- RNA Research and Splicing
- RNA modifications and cancer
- Insect behavior and control techniques
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Wnt/β-catenin signaling in development and cancer
- Insect Pest Control Strategies
- Advanced biosensing and bioanalysis techniques
- Digestive system and related health
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Enzyme Production and Characterization
- Transgenic Plants and Applications
- Connective tissue disorders research
- Lepidoptera: Biology and Taxonomy
- Developmental Biology and Gene Regulation
- Sirtuins and Resveratrol in Medicine
- Thyroid Cancer Diagnosis and Treatment
- Immune cells in cancer
- Mechanisms of cancer metastasis
- Insect Utilization and Effects
- Metabolism and Genetic Disorders
- Hippo pathway signaling and YAP/TAZ
- Insect Resistance and Genetics
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2024
Universidade do Porto
2007-2024
Instituto de Biologia Molecular e Celular
2014-2023
IPO Porto
2012-2015
Gene Therapy Laboratory
2014
Instituto de Investigaciones Biomédicas Sols-Morreale
2012
Universidad Autónoma de Madrid
2012
Abstract The phosphatidylinositol 3′-kinase (PI3K) pathway is frequently activated in thyroid carcinomas through the constitutive activation of stimulatory molecules (e.g., Ras) and/or loss expression function inhibitory PTEN protein that results Akt activation. Recently, it has been reported somatic mutations within PI3K catalytic subunit, PIK3CA, are common (25-40%) among colorectal, gastric, breast, ovarian cancers, and high-grade brain tumors. Moreover, PIK3CA have a tendency to cluster...
Objective: Our aim was to characterize the molecular and genotypic profile of eight thyroid carcinoma–derived cell lines—TPC1, FB2, B-CPAP, K1, XTC-1, C643, 8505C, Hth74—in order use them as in vitro models carcinogenesis. Design: We evaluated expression five thyroid-specific genes (Tg, TSHr, TPO, PAX8, TTF-1) establish lineage assess differentiation status each lines. screened for mutations most relevant oncogenes/tumor suppressor affected carcinogenesis: RAS, BRAF, CTNNB1, TP53 along with...
// Sara Duarte-Pereira 1 , Isabel Pereira-Castro 1,6 Sarah S. Silva Mariana Gonçalves Correia Célia Neto Luís Teixeira da Costa 1,2 António Amorim 1,3,4 and Raquel M. 1,5 IPATIMUP - Institute of Molecular Pathology Immunology the University Porto, Portugal 2 ICAAM Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Évora, 3 Faculty Sciences, 4 Investigação Inovação em Saúde, Universidade do 5 for...
Purpose: Germline SDHB, SDHC, and/or SDHD mutations have been reported in familial and apparently sporadic paragangliomas (PGLs). There is, however, some variation the prevalence, penetrance, phenotypic expression of succinate dehydrogenase (SDH) mutated gene among different populations. We sought to determine whether germline play a role cervical PGLs from northern Spain, where this disorder is particularly frequent, there any difference with respect data published other
T lymphocytes stimulated through their antigen receptor (TCR) preferentially express mRNA isoforms with shorter 3´ untranslated regions (3´-UTRs) derived from alternative pre-mRNA cleavage and polyadenylation (APA). However, the physiological relevance of APA programs remains poorly understood. CD5 is a T-cell surface glycoprotein that negatively regulates TCR signaling onset activation. plays pivotal role in mediating outcomes cell survival or apoptosis, may prevent both autoimmunity...
Macrophages are essential cells of the immune system that alter their inflammatory profile depending on microenvironment. Alternative polyadenylation in 3'UTR (3'UTR-APA) and intronic (IPA) mechanisms modulate gene expression, particularly cancer activated cells. Yet, how polarization colorectal (CRC) affect 3'UTR-APA IPA primary human macrophages was unclear.In this study, we isolated monocytes from healthy donors, differentiated polarized them into a pro-inflammatory state performed...
Abstract NET family members have recently emerged as important players in the development of multiple structures, from trachea fly larvae to vertebrate eye and human breast cancers. However, their mechanisms action are still poorly understood, we lack a detailed characterization functional domains, well gene expression patterns—particularly adult mammals. Here, present NLZ1/ZNF703 (NocA‐like zinc finger 1/Zinc 703), one two member genes. We show that is ubiquitously expressed mouse tissues,...
The olive fly, Bactrocera oleae (Rossi, 1790) (Diptera: Tephritidae), is the most important pest of trees globally, causing losses that, in absence control measures, can exceed 90% crop.In Mediterranean basin, where overwhelming majority production concentrated (~ 98%), organophosphate insecticides (OPs) have been main tool for B. last four decades, leading to development resistance these compounds.Mutations Ace gene, which codes acetylcholinesterase, target enzyme OPs and other...
Abstract The NET (for NocA, Nlz, Elbow, TLP-1) protein family is a group of conserved zinc finger proteins linked to embryonic development and recently associated with breast cancer. members this act as transcriptional repressors interacting both class I histone deacetylases Groucho/TLE co-repressors. In Drosophila, the Elbow NocA are vital for tracheae, eyes, wings legs, whereas in vertebrates ZNF703 ZNF503 important nervous system, eyes limbs. Despite relevance embryogenesis cancer, many...
The olive fly, Bactrocera oleae, is the most important pest affecting industry, to which it estimated cause average annual losses in excess of one billion dollars. As with other insects a wide distribution, generally accepted that understanding B. oleae population structure and dynamics fundamental for design implementation effective monitoring control strategies. However, despite advances past decade, clear picture oleae's still lacking. In Mediterranean basin, where more than 95%...
Prays oleae is one of the most important olive tree pests and a species interest in evolutionary studies, as it belongs to oldest extant superfamilies Ditrysian Lepidoptera. We determined its mitogenome sequence, found has common features for Lepidoptera, e.g. an >80% A + T content, apparent CGA start codon COX1 ATAGA(T)n motif control region, which also contains several copies 163-164 bp repeat. Importantly, displays Met-Ile-Gln tRNA gene order typical Ditrysia, consistent with hypothesis...
Transcriptional control by TCF/LEF proteins is crucial in key developmental processes such as embryo polarity, tissue architecture and cell fate determination. TCFs associate with β-catenin to activate transcription the presence of Wnt signaling, but its absence act repressors together Groucho-family (GRGs). TCF4 critical vertebrate intestinal epithelium, where TCF4-β-catenin complexes are necessary for maintenance a proliferative compartment, their abnormal formation initiates...
The host cytoskeleton is a major target for bacterial pathogens during infection. In particular, usurp the actin function to strongly adhere cell surface, induce plasma membrane remodelling allowing invasion and spread from disseminate whole organism. Keratins are cytoskeletal proteins that components of intermediate filaments in epithelial cells however, their role infection has been disregarded. Here we investigate keratins, keratins 8 18 (K8 K18), cellular by Listeria monocytogenes. We...
Mutations in the untranslated regulatory regions of genes may result abnormal gene expression or transcriptional regulation. In this study, we characterize ornithine transcarbamylase (OTC) mRNA isoforms X-linked OTC involved urea formation liver. Our data revealed that two major transcripts (OTC-t1 and OTC-t2) are more highly expressed than any other all tissues analyzed, though a longer transcript (OTC-t3) was also isolated characterized from brain sample. The OTC-t2 sequence fully matches...
Summary Macrophages are essential cells of the immune system that alter their inflammatory profile depending on microenvironment. Alternative polyadenylation in 3’UTR (3’UTR-APA) and intronic (IPA) mechanisms modulate gene expression, particular cancer activated cells. Yet, how polarization colorectal (CRC) microenvironment affect 3’UTR-APA IPA primary human macrophages remains unknown. Here, monocytes were isolated from healthy donors, differentiated polarized into a pro-inflammatory state...
The pinewood nematode (PWN) Bursaphelenchus xylophilus is the causative agent of pine wilt disease and greatest biological threat to conifer forests worldwide. Here we describe near-complete mitochondrial DNA (mtDNA) sequence (12,945 bp) PWN lineage recently introduced in Europe. absence polymorphisms across mtDNA three Portuguese isolates suggests that a single was southwestern We also found have an incomplete stop codon (TA) at COX3, while reference from South Korean isolate has complete...