A5079680190- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Malaria Research and Control
- Mosquito-borne diseases and control
- Calcium signaling and nucleotide metabolism
- Biochemical and Molecular Research
- Synthesis and Biological Evaluation
- Diphtheria, Corynebacterium, and Tetanus
- Parasites and Host Interactions
- Sirtuins and Resveratrol in Medicine
- Botulinum Toxin and Related Neurological Disorders
- Leptospirosis research and findings
- HIV/AIDS drug development and treatment
- Toxin Mechanisms and Immunotoxins
- Lipid Membrane Structure and Behavior
- Drug Transport and Resistance Mechanisms
- RNA Research and Splicing
- Hemoglobinopathies and Related Disorders
- Mycobacterium research and diagnosis
- interferon and immune responses
- Hepatitis B Virus Studies
- Erythrocyte Function and Pathophysiology
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Invertebrate Immune Response Mechanisms
Universidade do Porto
2012-2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016-2024
CUF Porto Hospital
2023
Institut Pasteur
2010-2021
Instituto de Biologia Molecular e Celular
2008-2021
Universidade Lusófona
2019
Centro de Genética Clínica
2019
Hospital de Santa Maria
2012-2017
Agropolis International
2008
Institut de Recherche pour le Développement
2006-2008
Abstract Plasmodium sporozoites deposited in the skin following a mosquito bite must migrate and invade blood vessels to complete their development liver. Once bloodstream, arrest liver sinusoids, but molecular determinants that mediate this specific homing are not yet genetically defined. Here we investigate involvement of thrombospondin-related sporozoite protein (TRSP) process using knockout berghei parasites vivo bioluminescence imaging mice. Resorting assay, trsp were found similar...
Malaria infection starts when the sporozoite stage of Plasmodium parasite is injected into skin by a mosquito. Sporozoites are known to traverse host cells before finally invading hepatocyte and multiplying erythrocyte-infecting forms, but how sporozoites reach hepatocytes in liver role cell traversal (CT) remain unclear. We report first quantitative imaging study rodents. show that can cross sinusoidal barrier multiple mechanisms, targeting Kupffer (KC) or endothelial associated not with CT...
The first step of Plasmodium development in vertebrates is the transformation sporozoite, parasite stage injected by mosquito skin, into merozoites, that invades erythrocytes and initiates disease. current view that, mammals, this conversion occurs only inside hepatocytes. Here, we document sporozoites rodent-infecting merozoites skin mice. After bite, ∼50% parasites remain at 24 h ∼10% are developing epidermis dermis, as well immunoprivileged hair follicles where they can survive for weeks....
Experimental infections with visceral Leishmania spp. are frequently performed referring to stationary parasite cultures that comprised of a mixture metacyclic and non-metacyclic parasites often little regard time culture purification. This may lead misleading or irreproducible experimental data. It is known the maintenance in vitro results progressive loss virulence can be reverted by passage mammalian host. In present study, we aimed characterize comparing vivo infection immunological...
Leishmania infection consists in two sequential events, the host cell colonization followed by proliferation/dissemination of parasite. In this review, we discuss importance distinct sets molecules, secreted and/or surface and nonsecreted antigens. The immune response against antigens is noted establishment dissect contribution immunopathology associated with leishmaniasis, showing these panantigens during course infection. As a further example proteins belonging to different groups, include...
Objective: To overcome the limitation of bisnaphthalimidopropyldiaaminooctane (BNIPDaoct) low physiological solubility and potentially increase its efficiency against visceral leishmaniasis (VL), a delivery system based on poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles was developed. Materials & methods: BNIPDaoct–PLGA were prepared by nanoprecipitation characterized. Anti-Leishmania activity evaluated using in vitro vivo VL infection models. Results: successfully produced sized at...
The NAD(+)-dependent deacetylases, namely sirtuins, are involved in the regulation of a variety biological processes such as gene silencing, DNA repair, longevity, metabolism, apoptosis, and development. An enzyme from parasite Leishmania infantum that belongs to this family, LiSIR2RP1, is tubulin deacetylase an ADP-ribosyltransferase. This enzyme's involvement L. virulence survival underscores its potential drug target. Our search for selective inhibitors LiSIR2RP1 has led, first time,...
Abstract Ribose-5-phosphate isomerase (RPI) belongs to the non-oxidative branch of pentose phosphate pathway, catalysing inter-conversion D-ribose-5-phosphate and D-ribulose-5-phosphate. Trypanosomatids encode a type B RPI, whereas humans have structurally unrelated A, making RPIB worthy exploration as potential drug target. Null mutant generation in Leishmania infantum was only possible when an episomal copy gene provided latter retained both vitro vivo absence pressure. This suggests is...
Proteins of the SIR2 (Silent Information Regulator 2) family are characterized by a conserved catalytic domain that exerts unique NAD(+)-dependent deacetylase activity on histones and various other cellular substrates. Previous reports from us have identified Leishmania infantum gene encoding cytosolic protein termed LiSIR2RP1 (Leishmania SIR2-related 1) belongs to family. Targeted disruption one allele led decreased amastigote virulence, in vitro as well vivo. In present study, attempts...
AbstractPurpose: Multiple biochemical and molecular alterations occur in pancreatic cancer cells. In the present study, attempts were made for first time, to explore level of expression members histone deacetylase encoding genes (HDACs) four tumor cell lines: Panc-1, BxPC-3, SOJ-6 MiaPaCa-2; two non-related cells: Jurkat HeLa. Furthermore, we examined possible relationship between levels HDACs sensitivity/resistance HDAC inhibitors (TSA, Nicotinamide Sirtinol). Methods: We have used human...
Ribose 5-phosphate isomerase is an enzyme involved in the non-oxidative branch of pentose phosphate pathway, and catalyzes inter-conversion D-ribose D-ribulose 5-phosphate. Trypanosomatids, including agent African sleeping sickness namely Trypanosoma brucei, have a type B ribose-5-phosphate isomerase. This absent from humans, which structurally unrelated ribose A, therefore has been proposed as attractive drug target waiting further characterization. In this study, brucei showed vitro...