A5091678548- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Insect and Pesticide Research
- Vector-borne infectious diseases
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- Calcium signaling and nucleotide metabolism
- Dermatological diseases and infestations
- Antimicrobial Peptides and Activities
- Insects and Parasite Interactions
- Malaria Research and Control
- Insect behavior and control techniques
- Forensic Entomology and Diptera Studies
- Helminth infection and control
- Sirtuins and Resveratrol in Medicine
- Insect Pest Control Strategies
- Morphological variations and asymmetry
- Biochemical and Molecular Research
- Diptera species taxonomy and behavior
- Biochemical and Structural Characterization
- Lipid Membrane Structure and Behavior
- Adenosine and Purinergic Signaling
- Species Distribution and Climate Change
Institut de Recherche pour le Développement
2016-2025
Université de Montpellier
2016-2025
Maladies Infectieuses et Vecteurs: Écologie, Génétique, Évolution et Contrôle
2015-2025
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2015-2024
Biologie et Génétique des Interactions Plante-Parasite
2011-2023
Interactions hotes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides
2011-2023
Agropolis International
2006-2023
Centre National de la Recherche Scientifique
2012-2023
École Nationale Supérieure de l'Électronique et de ses Applications
2023
CY Cergy Paris Université
2023
Background The recent geographical expansion of phlebotomine vectors Leishmania infantum in the Mediterranean subregion has been attributed to ongoing climate changes. At these latitudes, activity sand flies is typically seasonal; because seasonal phenomena are also sensitive general variations climate, current phenological data sets can provide a baseline for continuing investigations on fly population dynamics that may impact future scenarios leishmaniasis transmission. With this aim,...
Using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide microassay, previously described as means of quantifying Leishmania amazonensis in vitro at the amastigote stage (D. Sereno and J. L. Lemesre, Parisitol. Res., press), we have compared activities seven drugs, including those currently used to treat leishmaniasis, against axenically grown promastigote forms three species (L. amazonensis, mexicana, infantum, responsible for diffuse cutaneous, visceral respectively). The...
The ultimate stage of the transmission Dengue Virus (DENV) to man is strongly dependent on crosstalk between virus and immune system its vector Aedes aegypti (Ae. aegypti). Infection mosquito's salivary glands by DENV final step prior viral transmission. Therefore, in present study, we have determined modulatory effects infection response this organ carrying out a functional genomic analysis uninfected female Ae. mosquitoes infected with DENV. We shown that up-regulates expression genes...
ABSTRACT The mechanism(s) of activity pentavalent antimony [Sb(V)] is poorly understood. In a recent study, we have shown that potassium antimonyl tartrate, trivalent antimonial [Sb(III)], was substantially more potent than Sb(V) against both promastigotes and axenically grown amastigotes three Leishmania species, supporting the idea an in vivo metabolic conversion into Sb(III). We report infantum cultured under axenic conditions were susceptible to meglumine [Glucantime; Sb(V)], unlike...
The basic treatment of leishmaniasis consists in the administration pentavalent antimonials. mechanisms that contribute to antimonial toxicity against intracellular stage parasite (i.e., amastigote) are still unknown. In this study, combined use several techniques including DNA fragmentation assay and situ cytofluorometry terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling methods YOPRO-1 staining allowed us demonstrate potassium antimonyl tartrate, an...
Abstract The ability to manipulate the Leishmania genome create genetically modified parasites by introducing or eliminating genes is considered a powerful alternative for developing new generation vaccine against leishmaniasis. Previously, we showed that deletion of one allele infantum silent information regulatory 2 (LiSIR2) locus was sufficient dramatically affect amastigote axenic proliferation. Furthermore, LiSIR2 single knockout (LiSIR2+/−) amastigotes were unable replicate in vitro...
Rapid, accurate and high-throughput identification of vector arthropods is paramount importance in surveillance programmes that are becoming more common due to the changing geographic occurrence extent many arthropod-borne diseases. Protein profiling by MALDI-TOF mass spectrometry fulfils these requirements for identification, reference databases have recently been established several taxa, mostly with specimens from laboratory colonies. We validated a database containing 20 phlebotomine...
Antimicrobial peptides (AMPs) are promising drugs to kill resistant pathogens. In contrast bacteria, protozoan parasites, such as Leishmania, were little studied. Therefore, the antiparasitic mechanism of AMPs is still unclear. this study, we sought get further insight into by focusing our attention on temporin-SHa (SHa), a small broad-spectrum AMP previously shown be active against Leishmania infantum. To improve activity, designed analogs SHa and compared antibacterial mechanisms. [K3]SHa...
ABSTRACT Resistance to leishmanial infections depends on intracellular parasite killing by activated host macrophages through the l- arginine-nitric oxide (NO) metabolic pathway. Here we investigate cell death process induced NO for protozoan Leishmania amazonensis . Exposure of amastigotes moderate concentrations NO-donating compounds (acidified sodium nitrite NaNO 2 or nitrosylated albumin) endogenous produced lipopolysaccharide gamma interferon treatment infected resulted in a dramatic...
Protocols for DNA electroporation in Leishmania promastigote cells are well established. More recently, vitro culture of axenic amastigotes became possible. We have established conditions transformation axenically grown infantum amastigotes. Parameters were systematically studied using luciferase-mediated transient transfection. Cell lines expressing stable luciferase activity then selected, and their ability to be used an drug screening procedure was determined. A model established,...