A5068221783- Research on Leishmaniasis Studies
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Trypanosoma species research and implications
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Evolution and Genetic Dynamics
- Advanced Chemical Sensor Technologies
- Vector-borne infectious diseases
- Advanced biosensing and bioanalysis techniques
- Neurogenetic and Muscular Disorders Research
- Hereditary Neurological Disorders
- Aging, Health, and Disability
- Invertebrate Immune Response Mechanisms
- Neurological diseases and metabolism
- Vector-Borne Animal Diseases
- Identification and Quantification in Food
- Botulinum Toxin and Related Neurological Disorders
- Forensic Entomology and Diptera Studies
- Insect Pheromone Research and Control
- Viral Infections and Vectors
- Historical and Modern Theater Studies
- Insect Utilization and Effects
The Pirbright Institute
2019-2025
Animal and Plant Health Agency
2023-2024
Instituto de Salud Carlos III
2013-2023
Centro Nacional de Microbiologia
2013-2023
University of York
2022
Hospital Clínico San Carlos
2019
Background The recent geographical expansion of phlebotomine vectors Leishmania infantum in the Mediterranean subregion has been attributed to ongoing climate changes. At these latitudes, activity sand flies is typically seasonal; because seasonal phenomena are also sensitive general variations climate, current phenological data sets can provide a baseline for continuing investigations on fly population dynamics that may impact future scenarios leishmaniasis transmission. With this aim,...
Abstract Aedes aegypti is the main vector of several major pathogens including dengue, Zika and chikungunya viruses. Classical mosquito control strategies utilizing insecticides are threatened by rising resistance. This has stimulated interest in new genetic systems such as gene drivesHere, we test regulatory sequences from Ae. benign gonial cell neoplasm ( bgcn ) homolog to express Cas9 a separate multiplexing sgRNA-expressing cassette inserted into kynurenine 3-monooxygenase kmo gene. When...
CRISPR/Cas9-based homing gene drives have emerged as a potential new approach to mosquito control. While attempts been made develop such systems in Aedes aegypti, none able match the high drive efficiency observed Anopheles species. Here we generate Ae. aegypti transgenic lines expressing Cas9 using germline-specific regulatory elements and assess their ability bias inheritance of an sgRNA-expressing element (kmo
Abstract Background Anopheles stephensi is a competent malaria vector mainly present in southern Asia and the Arabian Peninsula. Since 2012, it has invaded several countries of eastern Africa, creating an emerging risk urban transmission. Urgent efforts are required to develop novel more efficient strategies for targeted control. CRISPR/Cas9-based homing gene drives have been proposed as attractive alternative strategies. Gene potential spread desired trait through population at higher rates...
An unusual increase of human leishmaniasis cases due to Leishmania infantum is occurring in an urban area southwestern Madrid, Spain, since 2010. Entomological surveys have shown that Phlebotomus perniciosus the only potential vector. Direct xenodiagnosis hares (Lepus granatensis) and rabbits (Oryctolagus cuniculus) collected focus proved they can transmit parasites colonized P. perniciosus. Isolates were characterized as L. infantum. The aim present work was conduct a comprehensive study...
Abstract CRISPR/Cas gene drives can bias transgene inheritance through different mechanisms. Homing are designed to replace a wild-type allele with copy of drive element on the homologous chromosome. In Aedes aegypti , sex-determining locus is closely linked white gene, which was previously used as target for homing ( w GDe ). Here, an analysis using this linkage we show that in males did not occur by homing, rather increased propagation donor element. We test same transgenes expressing Cas9...
Background Phlebotomus (Larroussius) perniciosus and Canis familiaris are respectively the only confirmed vector reservoir for transmission of Leishmania (L.) infantum MON-1 in Tunisia. However, hosts two other zymodemes, MON-24 MON-80, still unknown. The aim this study was to analyze L. life cycle a Tunisian leishmaniasis focus. For purpose, we have focused on: i) detection, quantification identification among sand fly population, ii) analysis blood meal preferences Larroussius (Lar.)...
Background An outbreak of human leishmaniasis due to Leishmania infantum has been registered in an urban area southwestern Madrid, Spain, since 2010. Entomological surveys carried out the municipalities Fuenlabrada, Leganés, Getafe and Humanes de Madrid showed that Phlebotomus perniciosus is only potential vector. In this work, intensive molecular surveillance was performed P . females captured region between 2012 2018. Methodology/Principal findings A total 1805 were analyzed for infection,...
A human leishmaniasis outbreak is occurring in the Madrid region, Spain, with parasite and vector involved being Leishmania infantum Phlebotomus perniciosus respectively. The aim of this study was to investigate virulence L. isolates from focus using a natural transmission model. Hamsters were infected by intraperitoneal inoculation (IP) or bites sand flies experimentally obtained P. collected area (IPER/ES/2012/BOS1FL1 IPER/ES/2012/POL2FL6) well characterized strain JPCM5...
Phlebotomine sand flies are the only known vectors of Leishmania spp. protozoan which causes leishmaniasis in 98 countries. In Spain, 11 fly species described, but Phlebotomus perniciosus and ariasi proven disease. On other hand, Sergentomyia minuta is one most abundant ubiquitous this territory, although scarce information available about species. Sand from genus for their preference to feed on cold-blooded animals traditionally involved transmission reptile Leishmania. However, studies...
The increasing prevalence of insecticide resistance and the ongoing global burden vector-borne diseases have encouraged new efforts in mosquito control. For Aedes aegypti, most important arboviral vector, integration rates achieved Cas9-based knock-ins so far been rather low, highlighting need to understand gene conversion patterns other factors that influence homology-directed repair (HDR) events this species. In study, we report effects sequence mismatches or donor template forms on rates....
Abstract Aedes aegypti , the yellow fever mosquito, is main vector of several major pathogens including fever, dengue, Zika and chikungunya viruses. Classical mosquito control strategies, mainly utilizing insecticides, have had success in controlling other vectors recent years, but are much less useful against Ae. even these methods threatened by rising insecticide resistance. This has stimulated interest new mechanisms, notably genetic systems such as gene drives. However, development...
ABSTRACT RNA guided CRISPR gene drives have shown the capability of biasing transgene inheritance in multiple species. Among these, homing endonuclease are most developed. In this study, we report functioning sds3, bgcn , and nup50 expressed Cas9 an Aedes aegypti split drive system targeting white gene. We their capability, propensity for maternal deposition, zygotic/somatic expression. Additionally, by making use tight linkage to sex-determining locus, were able elucidate mechanisms bias....
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Abstract Anopheles stephensi Liston, 1901 (Diptera: culicidae) is a competent vector of Plasmodium falciparum (Haemosporida: plasmodiidae) malaria, and its expansion in the African continent concern due to viability urban settings resistance insecticides. To enhance genetic tractability, we determined utility ~2 kb An. lipophorin ( lp ) promoter fragment driving transgene expression. Lipophorin genes are involved lipid transport insects, an orthologous gambiae (AGAP001826) was previously...