A5003308187- Malaria Research and Control
- Mosquito-borne diseases and control
- HIV Research and Treatment
- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Hepatitis B Virus Studies
- Toxoplasma gondii Research Studies
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Vector-borne infectious diseases
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- Trypanosoma species research and implications
- Monoclonal and Polyclonal Antibodies Research
- Invertebrate Immune Response Mechanisms
- Diffusion and Search Dynamics
- Complement system in diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Burkholderia infections and melioidosis
- Antibiotic Resistance in Bacteria
- Prion Diseases and Protein Misfolding
- Toxin Mechanisms and Immunotoxins
- Bacterial biofilms and quorum sensing
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
Centre National de la Recherche Scientifique
2019-2025
Institut Pasteur
2014-2025
Université Paris Cité
2022-2025
Inserm
2025
Sorbonne Université
2025
Centre d'Immunologie et des Maladies Infectieuses
2025
Institute of Infection and Immunity
2025
Immunité et Cancer
2023
Otto-von-Guericke University Magdeburg
2015-2021
Malaria infection starts when the sporozoite stage of Plasmodium parasite is injected into skin by a mosquito. Sporozoites are known to traverse host cells before finally invading hepatocyte and multiplying erythrocyte-infecting forms, but how sporozoites reach hepatocytes in liver role cell traversal (CT) remain unclear. We report first quantitative imaging study rodents. show that can cross sinusoidal barrier multiple mechanisms, targeting Kupffer (KC) or endothelial associated not with CT...
The virulence of intracellular pathogens such as Leishmania major (L. major) relies largely on their ability to undergo cycles replication within phagocytes, release, and uptake into new host cells. While all these steps are critical for successful establishment infection, neither the cellular niche efficient proliferation, nor spread cells have been characterized in vivo. Here, using a biosensor measuring pathogen proliferation living tissue, we found that monocyte-derived Ly6C+CCR2+...
Nitric oxide (NO) is an important antimicrobial effector but also prevents unnecessary tissue damage by shutting down the recruitment of monocyte-derived phagocytes. Intracellular pathogens such as Leishmania major can hijack these cells a niche for replication. Thus, NO might exert containment restricting availability cellular required efficient pathogen proliferation. However, indirect modes action remain to be established. By combining mathematical modeling with intravital 2-photon...
Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent additionally neutralize liver. Here, using a rodent malaria model, microscopy...
Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent additionally neutralize liver. Here, using a rodent malaria model, microscopy...
Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies (Abs) in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent Abs additionally neutralize liver. Here, using a rodent malaria model,...
The uptake and digestion of host hemoglobin by malaria parasites during blood-stage growth leads to significant oxidative damage membrane lipids. Repair lipid peroxidation is crucial for parasite survival. Here, we demonstrate that Plasmodium falciparum imports a antioxidant enzyme, peroxiredoxin 6 (PRDX6), from the red blood cell cytosol. PRDX6 lipid-peroxidation repair enzyme with phospholipase A2 (PLA2) activity. Inhibition PLA2 inhibitor, Darapladib, increases in disrupts transport...
Plasmodium sporozoites actively migrate in the dermis and enter blood vessels to infect liver. Despite their importance for malaria infection, little is known about these cutaneous processes. We combine intravital imaging a rodent model statistical methods unveil parasite strategy reach bloodstream. determine that display high-motility mode with superdiffusive Lévy-like pattern optimize location of scarce targets. When encountering vessels, frequently switch subdiffusive low-motility...
Human monoclonal antibodies (hmAbs) targeting the Plasmodium falciparum circumsporozoite protein (PfCSP) on sporozoite surface are a promising tool for preventing malaria infection. However, their mechanisms of protection remain unclear. Here, using 13 distinctive PfCSP hmAbs, we provide comprehensive view how hmAbs neutralize sporozoites in host tissues. Sporozoites most vulnerable to hmAb-mediated neutralization skin. rare but potent additionally blood and liver. Efficient tissues mainly...
Yeasts are largely used as bioreactors for vaccine production. Usually, antigens produced in yeast then purified and mixed with adjuvants before immunization. However, the purification costs safety concerns recently raised by use of new argue alternative strategies. To this end, whole both production delivery system appears attractive. Here, we evaluated Pichia pastoris an circumsporozoite protein (CS) Plasmodium, etiologic agent malaria. The CS from Plasmodium berghei (Pb) was selected...
Plasmacytoid dendritic cells (pDCs) are innate sensors of viral infections and important mediators antiviral immunity through their ability to produce large amounts IFN-α. Moreover, Toll-like receptor 7 (TLR7) 9 (TLR9) ligands, such as HIV CpG respectively, turn pDCs into TRAIL-expressing killer able lyse HIV-infected CD4+ T cells. NK can regulate by modulating pDC functions, production IFN-α well cell–cell contact is required promote cell functions. Impaired pDC-NK crosstalk was reported in...
Abstract During transmission of malaria‐causing parasites from mosquitoes to mammals, Plasmodium sporozoites migrate rapidly in the skin search for a blood vessel. The high migratory speed and narrow passages taken by suggest considerable strain on maintain their shape. Here, we show that membrane‐associated protein, concavin, is important maintenance sporozoite shape inside salivary glands during migration skin. Concavin‐GFP localizes at cytoplasmic periphery concavin( − ) progressively...
Following the RTS,S malaria vaccine, which showed only partial protection with short-term memory, there is strong support to develop second-generation vaccines that yield higher efficacy longer duration. The use of replicating viral vectors deliver subunit great interest due their capacity induce efficient cellular immune responses and long-term memory. measles vaccine virus offers an safe live vector could easily be implemented in field. Here, we produced recombinant viruses (rMV)...
Abstract Upon the onset of inflammatory responses, bacterial pathogens are confronted with altered tissue microenvironments which can critically impact on their metabolic activity and growth. Changes in these parameters have however remained difficult to analyze over time, would be critical dissect interplay between host immune response pathogen physiology. Here, we established an vivo biosensor for measuring growth rates Staphylococcus aureus ( S . ) a single cell-level days ongoing...
Introduction Plasmodium sporozoites (SPZ) inoculated by Anopheles mosquitoes into the skin of mammalian host migrate to liver before infecting hepatocytes. Previous work demonstrated that early production IL-6 in is detrimental for parasite growth, contributing acquisition a long-lasting immune protection after immunization with live attenuated parasites. Methods Considering as critical pro-inflammatory signal, we explored novel approach whereby itself encodes murine gene. We generated...
Yeast cells represent an established bioreactor to produce recombinant proteins for subunit vaccine development. In addition, delivery of antigens directly within heat-inactivated yeast is attractive due the adjuvancy provided by cell. this study, Pichia pastoris lysates carrying nucleoprotein (N) from measles virus were evaluated as a novel platform deliver circumsporozoite surface antigen (CS) Plasmodium. When expressed in yeast, N protein auto-assembles into highly multimeric...
Abstract The identification of surface proteins the sporozoite stage malaria parasites important for infectivity could aid in improvement efficacy vaccines targeting pre-erythrocytic stages. Thus, we set out to disclose role secreted protein with an altered thrombospondin repeat (SPATR), which is highly expressed sporozoites. Previous studies showed essential function blood stages, while no was detected sporozoites despite high expression. To achieve downregulation expression maintaining...
The sole currently approved malaria vaccine targets the circumsporozoite protein—the protein that densely coats surface of sporozoites, parasite stage deposited in skin mammalian host by infected mosquitoes. However, this only confers moderate protection against clinical diseases children, impelling a continuous search for novel candidates. In work, we studied importance membrane-associated erythrocyte binding-like (MAEBL) infection Plasmodium sporozoites. Using transgenic parasites and live...
At an early stage of HIV-1 transmission, plasmacytoid dendritic cells (pDCs) and natural killer (NK) are recruited into mucosal tissues. pDCs the major source type I interferon (IFN-α), a powerful innate antiviral cytokine, strong pDC response is associated with spontaneous virus control. The contribution to licensing NK inducing immunity make them key player in phase infection. In contrast, chronic expression IFN-α was found be mediator for HIV pathogenesis. We addressed herein question...