A5103556564- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Monoclonal and Polyclonal Antibodies Research
- Xenotransplantation and immune response
- Hepatitis B Virus Studies
- Toxoplasma gondii Research Studies
- RNA Interference and Gene Delivery
- Malaria Research and Control
- Vector-borne infectious diseases
- Organ Transplantation Techniques and Outcomes
- Glycosylation and Glycoproteins Research
- Immune Response and Inflammation
- HIV/AIDS drug development and treatment
- Virus-based gene therapy research
- Toxin Mechanisms and Immunotoxins
- Biochemical and Molecular Research
- Escherichia coli research studies
- Bacillus and Francisella bacterial research
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
- Cardiac Ischemia and Reperfusion
Institut Pasteur
2005-2025
Institute of Infection and Immunity
2025
Université Paris Cité
2025
Inserm
2003-2016
Immunité et Cancer
2016
Fondation de l'Avenir
2007-2008
National Jewish Health
2007
University of Colorado Denver
2007
National Institute of Allergy and Infectious Diseases
2002
National Institutes of Health
2002
CTLs have the potential to attack tumors, and adoptive transfer of can lead tumor regression in mouse models human clinical settings. However, dynamics cell elimination during efficient T therapy is unknown, it unclear whether act directly by destroying cells or indirectly initiating recruitment innate immune that mediate damage. To address these questions, we report real-time imaging apoptosis vivo using intravital 2-photon microscopy a Förster resonance energy transfer-based (FRET-based)...
Anti-CD20 Ab therapy has proven successful for treating B cell malignancies and a number of autoimmune diseases. However, how anti-CD20 Abs operate in vivo to mediate depletion is not fully understood. In particular, the anatomical location, type effector cells, mechanism underlying remain uncertain. Here, we found that liver major site recirculation accounts decrease numbers observed secondary lymphoid organs. Using intravital imaging, established that, upon treatment, Kupffer cells (KCs)...
Antibodies normally do not cross the blood-brain barrier (BBB) and cannot bind an intracellular cerebral antigen. We demonstrate here for first time that a new class of antibodies can BBB without treatment. Camelids produce native homodimeric heavy-chain antibodies, paratope being composed single-variable domain called VHH. Here, we used recombinant VHH directed against human glial fibrillary acidic protein (GFAP), specific marker astrocytes. Only basic VHHs (e.g., pI=9.4) were able to in...
In the absence of thymopoiesis, T lymphocytes are nevertheless present, mainly in gut epithelium. Ontogeny extrathymic pathway and extent its involvement euthymic mice controversial. These questions have been addressed by assessing expression recombinase activating gene (RAG) through use green fluorescent protein RAG2 transgenic mouse models. athymic mice, lymphopoiesis occurs mesenteric lymph node less Peyer's patches. Ontogenic steps this resemble those but with an apparent bias toward γδ...
The cellular mode of T cell priming in vivo remains to be characterized fully. We investigated the fate cell-dendritic (DC) interactions late phase activation lymph node. In general, CD4 cells detach from DCs before undergoing division. Using a new approach track history antigen (Ag)-recognition events, we demonstrated that activated/divided reengage different an Ag-specific manner. Two-photon imaging intact nodes suggested could establish prolonged with at multiple stages during process....
The regulation of T cell-dendritic cell (DC) contacts during clonal expansion is poorly defined. Although optimal CD4 responses require prolonged exposure to antigen (Ag), it believed that stable cell-DC interactions occur only the first day activation process. Here we show recently activated cells are in fact fully competent for establishing contact with Ag-bearing DC. Using two-photon imaging, found whereas between and DCs were infrequent at high precursor frequency, they readily observed...
Abstract Anti-CD20 monoclonal antibodies (mAbs) represent an effective treatment for a number of B cell malignancies and autoimmune disorders. Glycoengineering anti-CD20mAb may contribute to increased anti-tumor efficacy through enhanced antibody-dependent cellular cytotoxicity (ADCC) phagocytosis (ADP) as reported by in vitro studies. However, where how glycoengineered Ab potentiate therapeutic responses vivo is yet be elucidated. Here, we have performed mouse liver transplants demonstrate...
Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent additionally neutralize liver. Here, using a rodent malaria model, microscopy...
Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent additionally neutralize liver. Here, using a rodent malaria model, microscopy...
Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies (Abs) in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent Abs additionally neutralize liver. Here, using a rodent malaria model,...
The Gram-negative enteroinvasive bacterium Shigella flexneri is responsible for the endemic form of bacillary dysentery, an acute rectocolitis in humans. S. uses a type III secretion system to inject effector proteins into host cells, thus diverting cellular functions its own benefit. Protective immunity reinfection requires several rounds infection be elicited and short-lasting, suggesting that interferes with priming specific immunity. Considering key role played by T-lymphocyte...
Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located deep tissue environments. We describe a procedure visualize the behavior of natural killer (NK) and dendritic (DC) lymph nodes live, anesthetized mice. Intravital two-photon imaging is powerful tool study migration cell interactions immune such as NK DC physiological settings.
Abstract Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent additionally neutralize liver. Here, using a rodent malaria model,...