A5064958700- Malaria Research and Control
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Hepatitis B Virus Studies
- Vector-borne infectious diseases
- vaccines and immunoinformatics approaches
- Vibrio bacteria research studies
- Toxoplasma gondii Research Studies
- Parasites and Host Interactions
- Antimicrobial Peptides and Activities
- T-cell and B-cell Immunology
- Complement system in diseases
- Insect symbiosis and bacterial influences
- Drug Transport and Resistance Mechanisms
- Immunotherapy and Immune Responses
- Insect Resistance and Genetics
- Monoclonal and Polyclonal Antibodies Research
- Virology and Viral Diseases
- Diffusion and Search Dynamics
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
Immunité et Cancer
2017-2025
Institut Pasteur
2015-2025
Université Paris Cité
2021-2025
Institute of Infection and Immunity
2025
Centre National de la Recherche Scientifique
2019
University of Kentucky
2013
Universidade Federal de São Paulo
1995-2011
Bernhard Nocht Institute for Tropical Medicine
2006
Universität Hamburg
2006
University Medical Center Hamburg-Eppendorf
2006
The merozoite stage of the malaria parasite that infects erythrocytes and causes symptoms disease is initially formed inside host hepatocytes. However, mechanism by which hepatic merozoites reach blood vessels (sinusoids) in liver escape immune system before invading remains unknown. Here, we show parasites induce death detachment their hepatocytes, followed budding parasite-filled vesicles (merosomes) into sinusoid lumen. Parasites simultaneously inhibit exposure phosphatidylserine on outer...
Abstract Plasmodium sporozoites deposited in the skin following a mosquito bite must migrate and invade blood vessels to complete their development liver. Once bloodstream, arrest liver sinusoids, but molecular determinants that mediate this specific homing are not yet genetically defined. Here we investigate involvement of thrombospondin-related sporozoite protein (TRSP) process using knockout berghei parasites vivo bioluminescence imaging mice. Resorting assay, trsp were found similar...
Malaria infection starts when the sporozoite stage of Plasmodium parasite is injected into skin by a mosquito. Sporozoites are known to traverse host cells before finally invading hepatocyte and multiplying erythrocyte-infecting forms, but how sporozoites reach hepatocytes in liver role cell traversal (CT) remain unclear. We report first quantitative imaging study rodents. show that can cross sinusoidal barrier multiple mechanisms, targeting Kupffer (KC) or endothelial associated not with CT...
The first step of Plasmodium development in vertebrates is the transformation sporozoite, parasite stage injected by mosquito skin, into merozoites, that invades erythrocytes and initiates disease. current view that, mammals, this conversion occurs only inside hepatocytes. Here, we document sporozoites rodent-infecting merozoites skin mice. After bite, ∼50% parasites remain at 24 h ∼10% are developing epidermis dermis, as well immunoprivileged hair follicles where they can survive for weeks....
CD8 + T cells are specialized of the adaptive immune system capable finding and eliminating pathogen-infected cells. To date it has not been possible to observe destruction any pathogen by in vivo. Here we demonstrate a technique for imaging killing liver-stage malaria parasites bearing transgenic cell receptor specific parasite epitope. We report several features that have described vitro analysis process, chiefly formation large clusters effector around infected hepatocytes. The requires...
Calcium is a key signalling molecule in apicomplexan parasites and plays an important role diverse processes including gliding motility. Gliding essential for the malaria parasite to migrate from skin liver as well invade host tissues cells. Here we investigated dynamics of intracellular Ca(2+) motility Plasmodium berghei sporozoites by live imaging flow cytometry. We found that cytosolic levels increase when are activated suspension, which sufficient induce secretion integrin-like adhesins...
Malaria is caused by protozoan parasites of the genus Plasmodium and remains a global health concern. The parasite has highly adaptable life cycle comprising successive rounds asexual replication in vertebrate host sexual maturation mosquito vector Anopheles. Genetic manipulation been instrumental for deciphering function genes. Conventional reverse genetic tools cannot be used to study essential genes blood stages, thereby necessitating development conditional strategies. Among various such...
Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent additionally neutralize liver. Here, using a rodent malaria model, microscopy...
Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent additionally neutralize liver. Here, using a rodent malaria model, microscopy...
Plasmodium sporozoites are inoculated into the skin during bite of an infected mosquito. This motile stage invades cutaneous blood vessels to reach liver and infect hepatocytes. The circumsporozoite protein (CSP) on sporozoite surface is important antigen targeted by protective antibodies (Abs) in immunoprophylaxis or elicited vaccination. Antibody-mediated protection mainly unfolds parasite migration, but rare potent Abs additionally neutralize liver. Here, using a rodent malaria model,...
We have characterized a pore-forming lytic protein from the saliva of hematophagous insect Triatoma infestans, vector Chagas disease. This protein, named trialysin, has 22 kDa and is present in at about 200 microg/ml. Purified trialysin forms voltage-dependent channels planar lipid bilayers with conductance 880 +/- 40 pS. It lyses protozoan parasites bacteria indicating that it role control microorganism growth salivary glands. At higher concentrations, but below those found saliva, can also...
Preventing malaria infection through vaccination requires preventing every sporozoite inoculated by mosquito bite: a major challenge for Plasmodium falciparum. vivax sporozoites consist of tachysporozoites causing primary and bradysporozoites leading to relapses. We hypothesise that candidate P. vaccine with low efficacy against may substantially reduce transmission
Abstract In the malaria-causing parasite’s life cycle, Plasmodium sporozoites must travel from midgut of a mosquito to salivary glands before they can infect mammalian host. However, only fraction complete journey. Since gland invasion is required for transmission sporozoites, insights at molecular level contribute strategies malaria prevention. Recent advances in single-cell RNA sequencing provide an opportunity assess sporozoite heterogeneity resolution unattainable by bulk methods. this...