A5029728021- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Neurobiology and Insect Physiology Research
- Immune responses and vaccinations
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- Malaria Research and Control
- Insect Utilization and Effects
- Insect-Plant Interactions and Control
- Infectious Diseases and Mycology
- Antibiotic Resistance in Bacteria
- Olfactory and Sensory Function Studies
- Infectious Diseases and Tuberculosis
- Aquatic Invertebrate Ecology and Behavior
- IL-33, ST2, and ILC Pathways
- Insect and Arachnid Ecology and Behavior
- Hemoglobinopathies and Related Disorders
- vaccines and immunoinformatics approaches
- Health Systems, Economic Evaluations, Quality of Life
- Plant and soil sciences
- Erythrocyte Function and Pathophysiology
- Inflammatory Bowel Disease
- Vibrio bacteria research studies
- Hematological disorders and diagnostics
University of Utah
2022-2025
ARUP Laboratories (United States)
2024
Harvard University
2015-2022
Massachusetts General Hospital
2018-2019
Institut Pasteur
2011-2014
Yale University
2010-2011
A systematic functional analysis across much of the conventional Anopheles gambiae odorant receptor (AgOR) repertoire was carried out in Xenopus oocytes using two-electrode, voltage-clamp electrophysiology. The resulting data indicate that each AgOR manifests a distinct odor-response profile and tuning breadth. large diversity responses ranges from AgORs are responsive to single or small number odorants (specialists) more broadly tuned receptors (generalists). Several were identified respond...
Great progress has been made in the field of insect olfaction recent years. Receptors, neurons, and circuits have defined considerable detail, mechanisms by which they detect, encode, process sensory stimuli are being unraveled. We provide a guide to field, with special attention advances genetic model organism Drosophila . highlight key questions that merit additional investigation. then present our view how may be applied control disease-carrying insects such as mosquitoes, transmit...
Once considered a phenotypically monomorphic bacterium, there is growing body of work demonstrating heterogeneity among Mycobacterium tuberculosis (Mtb) strains in clinically relevant characteristics, including virulence and response to antibiotics. However, the genetic molecular basis for most phenotypic differences Mtb remains unknown. To investigate strain variation Mtb, we performed genome-wide transposon mutagenesis coupled with next-generation sequencing (TnSeq) panel clinical isolates...
For many pathogens, including most targets of effective vaccines, infection elicits an immune response that confers significant protection against reinfection. There has been debate as to whether natural Mycobacterium tuberculosis (Mtb) Here we experimentally assessed the conferred by concurrent Mtb in macaques, a robust experimental model human (TB), using combination serial imaging and challenge strains differentiated DNA identifiers. Strikingly, ongoing provided complete establishment...
Calcium is a key signalling molecule in apicomplexan parasites and plays an important role diverse processes including gliding motility. Gliding essential for the malaria parasite to migrate from skin liver as well invade host tissues cells. Here we investigated dynamics of intracellular Ca(2+) motility Plasmodium berghei sporozoites by live imaging flow cytometry. We found that cytosolic levels increase when are activated suspension, which sufficient induce secretion integrin-like adhesins...
Phenotypic antibiotic susceptibility testing in Mycobacterium tuberculosis is slow and cumbersome. Rapid molecular diagnostics promise to help guide therapy, but such assays rely on complete knowledge of the determinants altered susceptibility. Recent genomic studies antibiotic-resistant M. have identified several candidate loci beyond those already known contribute resistance; however, efforts provide experimental validation lagged. Our study identifies a gene (Rv0565c) that associated with...
There is growing evidence that genetic diversity in Mycobacterium tuberculosis, the causative agent of contributes to outcomes infection and public health interventions, such as vaccination. Epidemiological studies suggest among phylogeographic lineages M. strains belonging a sublineage Lineage 2 (mL2) are associated with concerning clinical features, including hypervirulence, treatment failure, vaccine escape. The global expansion increasing prevalence this has been attributed selective...
We successfully employed a single cell RNA sequencing (scRNA-seq) approach to describe the cells and communication networks characterizing granulomatous lymph nodes of TB patients. When mapping from individual patient samples, clustered based on their transcriptome similarities, we uniformly identify several types that known characterize human non-human primate granulomas. Whether high or low Mtb burden, find T cluster be one most abundant. Many expressing markers are clearly quantifiable...
Mycobacterium abscessus (MAB) is intrinsically resistant to many antibiotics, but the evolution of acquired drug resistance poorly understood. We analyzed published genomes 5,617 clinical MAB isolates from 20 countries and searched for signals ongoing in 35 drug-resistance-associated genes. Of these, we found 14 genes were subject positive selection identified novel mutational sites under selection. Among erm(41) V80I mutation arose exclusively strains with 28T affected 50.5% (1750/3465)...
Abstract Mycobacterium tuberculosis (Mtb) remains a global human health threat and significant cause of morbidity mortality. We document here the capture Mtb transcripts in libraries designed to amplify eukaryotic mRNA. These reads are often considered spurious or nuisance rarely investigated. Because early literature suggesting possible presence polyadenylated RNA, we included H37Rv reference genome when assembling scRNA seq from fine needle aspirate samples patients presenting at TB...
Mycobacterium tuberculosis (Mtb), the etiologic agent of (TB), remains a persistent global health challenge due to lack an effective vaccine. The only licensed TB vaccine, Bacille Calmette-Guerin (BCG), is live attenuated strain bovis, that protects young children from severe disease but fails provide protection through adulthood. It unclear why BCG-induced immunity provides incomplete despite inducing robust CD4+ T cell response, known critical correlate against Mtb. We set out interrogate...
For many pathogens, including most targets of effective vaccines, infection elicits an immune response that confers significant protection against reinfection. There has been debate as to whether natural M. tuberculosis (Mtb) Here we experimentally assessed the conferred by concurrent Mtb nfection in macaques, a robust experimental model human (TB), using combination serial imaging and challenge strains differentiated DNA identifiers. Strikingly, ongoing provided complete establishment...
ABSTRACT There is growing evidence that genetic diversity in Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, contributes to outcomes infection and public health interventions, such as vaccination. Epidemiological studies suggest among phylogeographic lineages Mtb, strains belonging Lineage 2 (L2) are associated with concerning clinical features including hypervirulence, treatment failure, vaccine escape. The global expansion increasing prevalence L2 has been attributed...