A5070411707- Escherichia coli research studies
- Urinary Tract Infections Management
- Gut microbiota and health
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Pelvic floor disorders treatments
- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- RNA and protein synthesis mechanisms
- Antibiotic Use and Resistance
- Bacterial Infections and Vaccines
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Pharmacological Effects of Natural Compounds
- Bladder and Urothelial Cancer Treatments
- Alzheimer's disease research and treatments
- Drug Transport and Resistance Mechanisms
- Diagnosis and treatment of tuberculosis
- Immunotherapy and Immune Responses
- Urinary Bladder and Prostate Research
- Probiotics and Fermented Foods
- Pediatric Urology and Nephrology Studies
- Biochemical and Structural Characterization
Harvard University
2018-2023
Washington University in St. Louis
2015-2020
Significance The evolution of multidrug resistance in pathogenic bacteria, including uropathogenic Escherichia coli (UPEC), that cause most urinary tract infections is becoming a worldwide crisis. UPEC use variety virulence factors and adhesins, the mannose-binding FimH adhesin, to colonize invade bladder tissue, often forming intracellular biofilms quiescent reservoirs can contribute recurrent recalcitrant treatment. Using two prototypical strains, we discovered positively selected residues...
Uropathogenic E. coli (UPEC), which cause urinary tract infections (UTI), utilize type 1 pili, a chaperone usher pathway (CUP) pilus, to UTI and colonize the gut. The pilus rod, comprised of repeating FimA subunits, provides structural scaffold for displaying tip adhesin, FimH. We solved 4.2 Å resolution structure rod using cryo-electron microscopy. Residues forming interactive surfaces that determine mechanical properties were maintained by selection based on global alignment fimA...
There is growing evidence that genetic diversity in Mycobacterium tuberculosis, the causative agent of contributes to outcomes infection and public health interventions, such as vaccination. Epidemiological studies suggest among phylogeographic lineages M. strains belonging a sublineage Lineage 2 (mL2) are associated with concerning clinical features, including hypervirulence, treatment failure, vaccine escape. The global expansion increasing prevalence this has been attributed selective...
Abstract Background Combatting the tuberculosis (TB) epidemic caused by Mycobacterium ( Mtb ) necessitates a better understanding of factors contributing to patient clinical outcomes and transmission. While host environmental have been evaluated, impact genetic background phenotypic diversity is underexplored. Previous work has made associations between lineages some epidemiological features, but bacterial traits underlying these connections are largely unknown. Methods We developed...
Urinary tract infections (UTI) affect over 150 million individuals worldwide every year. These are associated with significant morbidity and have a sizeable economic impact, $5 billion being spent on UTI treatment in the USA annually. Uropathogenic E. coli (UPEC) responsible for 80% of community aquired UTIs 65% nosocomial UTI. The current standard care is antibiotic therapy. However, 30–50% women experience recurrent (rUTI) despite receiving prevalence single multi‐drug resistant UPEC...
Adhesion to biotic and abiotic surfaces is an essential step in the infectious cycle of many human pathogens. To this end, both Gram‐positive Gram‐negative bacteria have developed a variety strategies overcome innate clearance mechanisms persist within host. The expression biogenesis Chaperone‐Usher Pathway (CUP) pili represent one such strategy. In addition mediating formation bacterial biofilms, these proteinaceous extracellular appendages contain, at their tip, two‐domain adhesin that...
ABSTRACT There is growing evidence that genetic diversity in Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, contributes to outcomes infection and public health interventions, such as vaccination. Epidemiological studies suggest among phylogeographic lineages Mtb, strains belonging Lineage 2 (L2) are associated with concerning clinical features including hypervirulence, treatment failure, vaccine escape. The global expansion increasing prevalence L2 has been attributed...