A5018112280- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- RNA and protein synthesis mechanisms
- Data-Driven Disease Surveillance
- CRISPR and Genetic Engineering
- Infectious Diseases and Tuberculosis
Oxford University Clinical Research Unit
2023-2024
Harvard University
2024
Liverpool School of Tropical Medicine
2024
Nepal Medical College Teaching Hospital
2024
Massachusetts General Hospital
2024
Wellcome Trust
2024
Health and Human Development (2HD) Research Network
2024
Hospital for Tropical Diseases
2023-2024
Center for Systems Biology
2024
University of California, San Francisco
2024
<ns3:p>Background Collecting and storing large number of sputum samples with a view to culturing these in the future requires an efficient initial handling method. We devised modified digestion decontamination method that maximised storage capacity <ns3:italic>Mycobacterium tuberculosis</ns3:italic> (M.tb) recovery from culture while minimising laboratory workload risk contamination. Methods collected smear microscopy positive patients pulmonary tuberculosis (TB). The were split processed...
Offering patients with tuberculosis (TB) an optimal and timely treatment regimen depends on the rapid detection of Mycobacterium (Mtb) drug resistance from clinical samples. Finding Low Abundance Sequences by Hybridization (FLASH) is a technique that harnesses efficiency, specificity, flexibility Cas9 enzyme to enrich targeted sequences.
ABSTRACT Mortality from tuberculous meningitis (TBM) remains around 30%, with most deaths occurring within 2 months of starting treatment. drug-resistant strains is higher still, making early detection drug resistance (DR) essential. Targeted next-generation sequencing (tNGS) produces high read depths, allowing the DR-associated alleles low frequencies. We applied Deeplex Myc-TB—a tNGS assay—to cerebrospinal fluid (CSF) samples 72 adults microbiologically confirmed TBM and compared its...
Antibiotic tolerance in Mycobacterium tuberculosis reduces bacterial killing, worsens treatment outcomes, and contributes to resistance. We studied rifampicin isolates with or without isoniazid resistance (IR). Using a minimum duration of killing assay, we measured survival isoniazid-susceptible (IS, n=119) resistant (IR, n=84) isolates, correlating growth, inhibitory concentrations (MICs), isoniazid-resistant mutations. Longitudinal IR were analyzed for changes genetic variant emergence....
We conducted a descriptive analysis of multi-drug resistant tuberculosis (MDR-TB) in Vietnam's two largest cities, Hanoi and Ho Chi Minh city. All patients with rifampicin were recruited from surrounding provinces between 2020 2022. Additional city over the same time period. Demographic data recorded all patients, samples collected, cultured, whole genome sequenced analysed for drug resistance mutations. Genomic susceptibility predictions made on basis World Health Organization's catalogue...
We characterized the spatial distribution of drug-susceptible (DS) and multidrug-resistant (MDR) tuberculosis (TB) cases in Ho Chi Minh City, Vietnam, a major metropolis southeastern Asia, explored demographic socioeconomic factors associated with local TB burden. Hot spots DS MDR incidence were observed central parts substantial heterogeneity was across wards. Positive autocorrelation for both TB. Ward-level HIV prevalence male proportion population. No ward-level indicators case count...
Abstract Background Combatting the tuberculosis (TB) epidemic caused by Mycobacterium ( Mtb ) necessitates a better understanding of factors contributing to patient clinical outcomes and transmission. While host environmental have been evaluated, impact genetic background phenotypic diversity is underexplored. Previous work has made associations between lineages some epidemiological features, but bacterial traits underlying these connections are largely unknown. Methods We developed...
Collecting and storing large number of sputum samples with a view to culturing these in the future requires an efficient initial handling method. We devised modified digestion decontamination method that maximised storage capacity
Antibiotic tolerance in Mycobacterium tuberculosis leads to less effective bacterial killing, poor treatment responses and resistant emergence. Therefore, we investigated the rifampicin of M. isolates, with or without pre-existing isoniazid-resistance. We determined in-vitro survival fraction by minimum duration killing assay isoniazid susceptible (IS, n=119) (IR, n=84) isolates. Then correlated growth, inhibitory concentrations (MICs) isoniazid-resistant mutations. The longitudinal IR...
Antibiotic tolerance in Mycobacterium tuberculosis leads to less effective bacterial killing, poor treatment responses and resistant emergence. There is limited understanding of antibiotic clinical isolates M. . Therefore, we investigated the rifampicin isolates, with or without pre-existing isoniazid-resistance. In-vitro survival fractions determined by minimum duration killing assay isoniazid susceptible (n=119) (n=84) isolates. Rifampicin was correlated growth, inhibitory concentrations...
Antibiotic tolerance in Mycobacterium tuberculosis reduces bacterial killing, worsens treatment outcomes, and contributes to resistance. We studied rifampicin isolates with or without isoniazid resistance (IR). Using a minimum duration of killing assay, we measured survival isoniazid-susceptible (IS, n=119) resistant (IR, n=84) isolates, correlating growth, inhibitory concentrations (MICs), isoniazid-resistant mutations. Longitudinal IR were analyzed for changes genetic variant emergence....
Abstract Antibiotic tolerance in Mycobacterium tuberculosis leads to less effective bacterial killing, poor treatment responses and resistant emergence. Therefore, we investigated the rifampicin of M. isolates, with or without pre-existing isoniazid-resistance. We determined in-vitro survival fraction by minimum duration killing assay isoniazid susceptible (IS, n=119) (IR, n=84) isolates. Then correlated growth, inhibitory concentrations (MICs) isoniazid-resistant mutations. The longitudinal...
<ns3:p><ns3:bold>Background:</ns3:bold> Collecting and storing large number of sputum samples with a view to culturing these in the future requires an efficient initial handling method. We devised modified digestion decontamination method that maximised storage capacity <ns3:italic>Mycobacterium tuberculosis </ns3:italic>(M.tb)<ns3:italic> </ns3:italic>recovery from culture while minimising laboratory workload risk contamination.</ns3:p><ns3:p> <ns3:bold>Methods: </ns3:bold>We collected...