A5085297769- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- Computational Drug Discovery Methods
- SARS-CoV-2 and COVID-19 Research
- Complement system in diseases
- Viral Infections and Outbreaks Research
- Receptor Mechanisms and Signaling
- vaccines and immunoinformatics approaches
- Protein Degradation and Inhibitors
- CRISPR and Genetic Engineering
- PARP inhibition in cancer therapy
- Bacteriophages and microbial interactions
- Pluripotent Stem Cells Research
- Gene expression and cancer classification
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Animal Genetics and Reproduction
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- Trypanosoma species research and implications
University of Washington
2013-2020
Neoleukin Therapeutics (United States)
2020
We developed a de novo protein design strategy to swiftly engineer decoys for neutralizing pathogens that exploit extracellular host proteins infect the cell. Our pipeline allowed design, validation, and optimization of human angiotensin-converting enzyme 2 (hACE2) neutralize severe acute respiratory syndrome coronavirus (SARS-CoV-2). The best monovalent decoy, CTC-445.2, bound with low nanomolar affinity high specificity receptor-binding domain (RBD) spike protein. Cryo-electron microscopy...
While the cost of DNA sequencing has dropped by five orders magnitude in past decade, synthesis remains expensive for many applications. Although microarrays have decreased oligonucleotide synthesis, use array-synthesized oligos practice is limited short lengths, high error rates, low yield and challenges assembling long constructs from complex pools. Toward addressing these issues, we developed a protocol multiplex pairwise assembly To evaluate method, attempted to assemble up 2271 targets...
Broadly neutralizing antibodies targeting a highly conserved region in the hemagglutinin (HA) stem protect against influenza infection. Here, we investigate protective efficacy of protein (HB36.6) computationally designed to bind with high affinity same HA stem. We show that intranasal delivery HB36.6 affords protection mice lethally challenged diverse strains independent Fc-mediated effector functions or host antiviral immune response. This prevents infection when given as single dose 6.0...
ABSTRACT The crisis of biodiversity loss in the anthropogenic era requires new tools for studying non-model organisms. Elephants, example, are both an endangered species and excellent models complex phenotypes like size, social behavior, longevity, but they remain severely understudied. Here we report first derivation elephant ( Elephas maximus ) induced pluripotent stem cells (emiPSCs) achieved via a two-step process chemical-media induction colony selection, followed by overexpression...
Influenza is a ubiquitous and recurring infection that results in approximately 500 000 deaths globally each year. Commercially available rapid diagnostic tests are based upon detection of the influenza nucleoprotein, which limited they unable to differentiate by species require an additional viral lysis step. Sample preprocessing can be minimized or eliminated targeting intact virus, thereby reducing assay complexity leveraging large number hemagglutinin proteins on surface virus. Here, we...
There is an urgent need for the ability to rapidly develop effective countermeasures emerging biological threats, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes ongoing disease 2019 (COVID-19) pandemic. We have developed a generalized computational design strategy engineer de novo proteins precisely recapitulate protein surface targeted by agents, like viruses, gain entry into cells. The designed act decoys block cellular and aim be resilient viral...