A5016769340- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Bacterial Genetics and Biotechnology
- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Cancer Research and Treatments
- Advanced Biosensing Techniques and Applications
- Solar and Space Plasma Dynamics
- Animal Genetics and Reproduction
- Biotechnology and Related Fields
- Modular Robots and Swarm Intelligence
- Ionosphere and magnetosphere dynamics
- Toxin Mechanisms and Immunotoxins
- Zebrafish Biomedical Research Applications
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
Harvard University
2017-2024
Harvard University Press
2020-2022
Boston VA Research Institute
2020-2022
Boston University
2010-2014
Regulatory gaps must be filled before gene drives could used in the wild
We present a workflow for the design and production of biological networks from high-level program specifications. The is based on sequence intermediate models that incrementally translate specifications into DNA samples implement them. identify algorithms translating between adjacent them as set software tools, organized four-stage toolchain: Specification, Compilation, Part Assignment, Assembly. specification stage begins with Boolean logic computation specified in Proto programming...
ABSTRACT The crisis of biodiversity loss in the anthropogenic era requires new tools for studying non-model organisms. Elephants, example, are both an endangered species and excellent models complex phenotypes like size, social behavior, longevity, but they remain severely understudied. Here we report first derivation elephant ( Elephas maximus ) induced pluripotent stem cells (emiPSCs) achieved via a two-step process chemical-media induction colony selection, followed by overexpression...
The fast-paced field of synthetic biology is fundamentally changing the global biosecurity framework. Current regulations and strategies are based on previous governance paradigms for pathogen-oriented security, recombinant DNA research, broader concerns related to genetically modified organisms (GMOs). Many scholarly discussions practitioners therefore concerned that outpaces established biosafety measures prevent deliberate malicious or inadvertent accidental misuse biology's processes...
Abstract Human cell conversion technology has become an important tool for devising new transplantation therapies, generating disease models and testing gene therapies. However, while transcription factor over-expression-based methods have shown great promise in types vitro, they often endure low efficiency. In this context, effort been devoted to increasing the efficiency of current protocols development computational approaches can be help endeavor. Here we introduce a computer-guided...
Targeted delivery of therapeutic proteins toward specific cells and across cell membranes remains major challenges. Here, we develop protein-based systems utilizing detoxified single-chain bacterial toxins such as diphtheria toxin (DT) botulinum neurotoxin (BoNT)-like toxin, BoNT/X, carriers. The system can deliver large protein cargoes including Cas13a, CasRx, Cas9, Cre recombinase into in a receptor-dependent manner, although ribonucleoproteins containing guide RNAs is not successful....
A synthetic auxotroph does not escape from biocontainment after evolution, demonstrating that life always find a way.
The high variability of the intensity suprathermal electron flux in solar wind is usually ascribed to sources on Sun. Here we demonstrate that a substantial amount arises from peaks stream interaction regions, where fast runs into slow and creates pressure ridge at interface. Superposed epoch analysis centered interfaces 26 regions previously identified Wind data reveal twofold increase 250 eV (integrated over pitch angle). Whether result compression there or are signatures coronal hole...
Multicellular organisms originate from a single cell, ultimately giving rise to mature of heterogeneous cell type composition in complex structures. Recent work the areas stem biology and tissue engineering has laid major groundwork ability convert certain types cells into other types, but there been limited progress control morphology cellular masses as they grow. Contemporary approaches this problem have included use artificial scaffolds, 3D bioprinting, media formulations; however, are no...
iGEM has spent the past decade encouraging teams to push their projects frontiers of synthetic biology. However, as project complexity increases, so too does level assumed risk. In absence a coherent international framework for evaluating these risks in biology, recently engaged with MIT Program on Emerging Technologies develop progressive approach handling questions safety and security. These two groups have worked together create rigorous screening program, acknowledging that strengthened...
Abstract The ability to differentiate stem cells into human cell types is essential define basic mechanisms and therapeutics, especially for not routinely accessible by biopsies. But while engineered expression of transcription factors (TFs) identified through TF screens has been found rapidly efficiently produce some types, generation other that require complex combinations TFs elusive. Here we develop an iterative, pooled single-cell screening method improves the identification effective...
A bstract Multi-cellular organisms originate from a single cell, ultimately giving rise to mature of heterogeneous cell type composition in complex structures. Recent work the areas stem biology and tissue engineering have laid major groundwork ability convert certain types cells into other types, but there has been limited progress control morphology cellular masses as they grow. Contemporary approaches this problem included use artificial scaffolds, 3D bioprinting, media formulations,...
Owl (www.owlcad.org) is a biodesign automation tool that generates electronic datasheets for synthetic biological parts using common formatting. Data can be retrieved automatically from existing repositories and modified in the user interface (UI). uses data to generate an HTML page with standard typesetting saved as PDF file. Here we present software its alpha version, current UI, description of input generating datasheet, example datasheets, vision tool's role automation.
Abstract Molecular biologists rely on the use of fluorescent probes to take measurements their model systems. These fluorophores fall into various classes (e.g. dyes, proteins, etc.), but they all share some general properties (such as excitation and emission spectra, brightness) require similar equipment for data acquisition. Selecting an ideal set a particular measurement technology or vice versa is multidimensional problem that difficult solve with ad hoc methods due enormous solution...
We present a framework that allows us to construct and formally analyze the behavior of synthetic gene circuits from specifications in high level language used describing electronic circuits. Our back-end synthesis tool automatically generates genetic-regulatory network (GRN) topology realizing with assigned biological parts database. describe experimental procedures acquire characterization data for mathematical models capturing all possible behaviors generated GRN. delineate algorithms...
We present a framework that allows us to construct and formally analyze the behavior of synthetic gene circuits from specifications in high level language used describing electronic circuits. Our back-end synthesis tool automatically generates genetic-regulatory network (GRN) topology realizing with assigned biological "parts" database. describe experimental procedures acquire characterization data for parts mathematical models capturing all possible behaviors generated GRN. delineate...
A bstract The creation of induced pluripotent stem cells (iPSCs) has enabled scientists to explore the derivation many types cells. While there are diverse general approaches for cell-fate engineering, one fastest and most efficient is transcription factor (TF) over-expression. However, finding right combination TFs over-express differentiate iPSCs directly into other cell-types a difficult task. Here were describe machine-learning (ML) pipeline, called CellCartographer , using chromatin...