A5028210452- Biosensors and Analytical Detection
- Gold and Silver Nanoparticles Synthesis and Applications
- Lymphoma Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- bioluminescence and chemiluminescence research
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Viral-associated cancers and disorders
- Advanced Biosensing Techniques and Applications
- Chemical Synthesis and Analysis
- Protein Structure and Dynamics
- Nicotinic Acetylcholine Receptors Study
- Nanocluster Synthesis and Applications
- CNS Lymphoma Diagnosis and Treatment
- Adrenal and Paraganglionic Tumors
- Cholinesterase and Neurodegenerative Diseases
- Biotin and Related Studies
- Molecular Sensors and Ion Detection
- Cancer, Hypoxia, and Metabolism
- Enzyme Structure and Function
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Endometrial and Cervical Cancer Treatments
- Chronic Lymphocytic Leukemia Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
Ente Ospedaliero Cantonale
2024
University of Verona
2022-2024
Ospedaliera di Piacenza
2024
Guglielmo da Saliceto Hospital
2022-2023
École Polytechnique Fédérale de Lausanne
2011-2018
École Polytechnique
2013
NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry
2011
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used for imaging either toxic, require illumination with blue light or not compatible super-resolution microscopy, thereby limiting their utility. Here we describe a far-red stain, SiR-Hoechst, which displays minimal toxicity, is applicable different cell types and tissues, microscopy. The combination of these properties makes this probe powerful tool
Abstract We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no‐wash quantification of analytes. Our approach is based on genetic fusion antibody fragments NanoLuc luciferase and SNAP‐tag, latter being labeled with synthetic fluorescent competitor antigen. Binding antigen, here drugs, by displaces tethered from disrupts resonance energy transfer (BRET) between fluorophore. The semisynthetic sensors display tunable response range...
Abstract We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The are chimeras of NanoLuc with either SNAP‐tag or HaloTag7. Labeling the self‐labeling tag a fluorophore shifts maximum that fluorophore. Luciferases tunable colors have applications as reporter genes, for construction biosensors and in bioimaging.
Abstract The possibility to design proteins whose activities can be switched on and off by unrelated effector molecules would enable applications in various research areas, ranging from biosensing synthetic biology. We describe here a general method modulate the activity of protein response concentration specific effector. approach is based ligands that possess two mutually exclusive binding sites, one for interest Tethering such ligand results an intramolecular ligand–protein interaction...
Abstract Acetylcholine is a key neurotransmitter, and anticholinesterase agents are essential compounds used as medical drugs, pesticides, chemical warfare agents. A semisynthetic fluorescence‐based probe for the direct, real‐time detection of acetylcholine introduced. The possesses good sensitivity, tunable range, can be selectively targeted to cell surfaces, thereby making it an attractive tool applications in analytical chemistry quantitative biology.
The steroid hormone 17α-hydroxylprogesterone (17-OHP) is a biomarker for congenital adrenal hyperplasia and hence there considerable interest in development of sensors this compound. We used computational protein design to generate models with binding sites 17-OHP containing an extended, nonpolar, shape-complementary pocket the four-ring core compound, hydrogen bonding residues at base interact carbonyl hydroxyl groups more polar end ligand. Eight 16 designed proteins experimentally tested...
Two new water-soluble gold nanoparticles (AO-TEG-Au and AO-PEG-Au NPs) are prepared characterized. They stabilized by thioalkylated oligoethylene glycols functionalized with fluorescent Acridine Orange (AO) derivatives. Despite the different core sizes (11.8 3.9 nm respectively) shell composition, they both well dispersed stable in water, even if some self-aggregation is observed case of AO-TEG-Au NPs. However, NPs show much lower emission efficiency respect to Spectrophotometric...
Abstract Primary lymphoma of the female genital tract (PLFGT) is a rare type extranodal lymphoma. In this retrospective study from International Extranodal Lymphoma Study Group, we analyzed clinical data 60 women diagnosed with PLFGT between 1982 and 2012. The median age was 52 years. Limited stage, as defined by Ann Arbor FIGO staging systems, observed in 55% 63% cases, respectively. uterus primary site 25 ovaries second most common ( n = 24). histological subtype diffuse large B‐cell...
Abstract We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no‐wash quantification of analytes. Our approach is based on genetic fusion antibody fragments NanoLuc luciferase and SNAP‐tag, latter being labeled with synthetic fluorescent competitor antigen. Binding antigen, here drugs, by displaces tethered from disrupts resonance energy transfer (BRET) between fluorophore. The semisynthetic sensors display tunable response range...
Abstract We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The are chimeras of NanoLuc with either SNAP‐tag or HaloTag7. Labeling the self‐labeling tag a fluorophore shifts maximum that fluorophore. Luciferases tunable colors have applications as reporter genes, for construction biosensors and in bioimaging.
Head and neck paragangliomas (HNPGLs), rare chemoresistant tumors curable only with surgery, are strongly influenced by genetic predisposition, hence patients relatives require lifetime follow-up MRI and/or PET-CT because of de novo disease risk. This entails exposure to electromagnetic/ionizing radiation, costs, organizational challenges, scattered far from reference centers. Simplified first-line screening strategies needed. We employed flow injection analysis tandem mass spectrometry, as...
Abstract Acetylcholine is a key neurotransmitter, and anticholinesterase agents are essential compounds used as medical drugs, pesticides, chemical warfare agents. A semisynthetic fluorescence‐based probe for the direct, real‐time detection of acetylcholine introduced. The possesses good sensitivity, tunable range, can be selectively targeted to cell surfaces, thereby making it an attractive tool applications in analytical chemistry quantitative biology.
Abstract Attempts to create novel ligand-binding proteins often focus on formation of a binding pocket with shape complementarity against the desired ligand (particularly for compounds that lack distinct polar moieties). Although designed exhibit ligand, in some cases they display unintended recognition behavior. One such protein, was originally intended bind tetrahydrocannabinol (THC), found instead 25-hydroxy-cholecalciferol (25-D3) and subjected biochemical characterization, further...
The combination of chemotherapy and immunotherapy for metastatic cholangiocarcinoma (CCA) offers promising improvements in survival response rates beyond traditional treatments. TOPAZ-1 KEYNOTE-966 have demonstrated the efficacy combining (durvalumab pembrolizumab) with chemotherapy, even gallbladder cancer (GBC), a complete rate 2.7% trial. Advanced CCA treated combinations has shown responses influenced by high programmed death-ligand 1 (PD-L1) or Epstein-Barr virus expression. These were...
Mycobacterium abscessus complex, hereinafter Mab, is a taxonomic group of rapidly growing, nontuberculous mycobacteria (NTM). Despite major advances in understanding virulence, pathogenicity and mechanism antibiotic resistance, Mab remains significant cause pulmonary extra-pulmonary disease. Herein, we describe disseminated, macrolide-resistant, subspecies infection occurring severely immune-compromised 34-year-old allotransplanted female patient affected by chronic graft versus host disease...
Keywords: biosensor ; bioprobe luminescence acetylcholine imaging FRET These Ecole polytechnique federale de Lausanne EPFL, n° 6063 (2014)Programme doctoral Chimie et Genie chimiqueFaculte des sciences baseInstitut ingenierie chimiquesLaboratoire d'ingenierie proteinesJury: Prof. X. Hu (president du jury), K. Johnsson (directeur these), B. Fierz, M. Lochner, Dr E. Nakata (rapporteurs) Public defense: 2014-1-10 Reference doi:10.5075/epfl-thesis-6063Print copy in library catalog Record created...
Topic: 19. Aggressive Non-Hodgkin lymphoma - Clinical Background: Peripheral T-cell (PTCL), is a heterogeneous and rare entity (5-10% of all lymphomas). Central nervous system (CNS) involvement at diagnosis relapse in patients with PTCL ranged from 2-9%, well-known dismal prognosis. There are no clear recommendations for CNS prophylaxis PTCL, the exception some entity. We retrospectively collected clinicopathologic treatment data 205 patients, diagnosed between 2000 2022, who underwent...