A5011393275- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Calcium signaling and nucleotide metabolism
- Sirtuins and Resveratrol in Medicine
- Adenosine and Purinergic Signaling
- bioluminescence and chemiluminescence research
- Monoclonal and Polyclonal Antibodies Research
- Quantum Dots Synthesis And Properties
- Mitochondrial Function and Pathology
- Nanoparticles: synthesis and applications
- Microfluidic and Capillary Electrophoresis Applications
- Pharmacological Effects of Natural Compounds
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Tryptophan and brain disorders
- Innovative Microfluidic and Catalytic Techniques Innovation
- Magnolia and Illicium research
- Oxidative Organic Chemistry Reactions
- Heme Oxygenase-1 and Carbon Monoxide
- Molecular Communication and Nanonetworks
- Heavy Metal Exposure and Toxicity
- Electrochemical sensors and biosensors
- Drug Transport and Resistance Mechanisms
- Polymer Surface Interaction Studies
- Inflammasome and immune disorders
Shenzhen Institutes of Advanced Technology
2020-2024
Chinese Academy of Sciences
2022-2024
Shenzhen University
2024
Max Planck Institute for Medical Research
2017-2019
École Polytechnique Fédérale de Lausanne
2014-2018
NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry
2017
Wuhan University
2010-2014
State Key Laboratory of Virology
2010-2014
In-Q-Tel
2012
Monitoring metabolites at the point of care could improve diagnosis and management numerous diseases. Yet for most metabolites, such assays are not available. We introduce semisynthetic, light-emitting sensor proteins use in paper-based metabolic assays. The metabolite is oxidized by nicotinamide adenine dinucleotide phosphate, changes color presence reduced cofactor, enabling quantification with a digital camera. approach makes any that can be cofactor candidate quantitative point-of-care...
Zinc oxide nanoparticles (ZnO NPs) are increasingly applied in a diverse array of industrial and commercial products. Therefore, it is urgently required to characterize their toxic behavior. ZnO NPs have been reported induce effects at the levels individual organism, tissue, cell DNA. However, little known about potential impacts subcellular level. In present work, we investigated toxicity isolated rat liver mitochondria. We found that treatment mitochondria with resulted collapse...
Abstract We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no‐wash quantification of analytes. Our approach is based on genetic fusion antibody fragments NanoLuc luciferase and SNAP‐tag, latter being labeled with synthetic fluorescent competitor antigen. Binding antigen, here drugs, by displaces tethered from disrupts resonance energy transfer (BRET) between fluorophore. The semisynthetic sensors display tunable response range...
For efficient coupling of droplet-based microfluidics with mass spectrometry (MS), a spyhole drilled on the top microchip is used to sample passing droplets by electrostatic-spray ionization (ESTASI) MS. The technique involves placing an electrode below chip under and applying high-voltage pulses. Electrospray occurs directly from spyhole, droplet content analyzed MS without dilution or oil removal step. To demonstrate versatility this technique, we have successfully monitored tryptic...
Abstract We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The are chimeras of NanoLuc with either SNAP‐tag or HaloTag7. Labeling the self‐labeling tag a fluorophore shifts maximum that fluorophore. Luciferases tunable colors have applications as reporter genes, for construction biosensors and in bioimaging.
Abstract Along with the widespread development of their bioapplications, concerns about biosafety quantum dots (QDs) have increasingly attracted intensive attention. This study examines toxic effect and subcellular location cadmium telluride (CdTe) QDs different sizes against yeast Saccharomyces cerevisiae . The innovative approach is based on combination microcalorimetric, spectroscopic, electrochemical, microscopic methods, which allows analysis CdTe S. its mechanism. According to values...
Mapping NAD+ dynamics in live cells and human is essential for translating interventions into effective therapies. Yet, genetically encoded sensors with better specificity pH resistance are still needed the cost-effective monitoring of both subcellular compartments clinical samples. Here, we introduce multicolor, resonance energy transfer-based covering nano- to millimolar concentration ranges measurement visualization. The captured blood increase induced by NMN supplementation revealed...
Heme oxygenase-1 (HO-1, HMOX1) degrades heme protecting cells from heme-induced oxidative damage. Beyond its well-established cellular functions, has emerged as a stabilizer of G-quadruplexes. These secondary DNA structures interfere with replication. We recently revealed that nuclear HO-1 colocalizes G-quadruplexes and promotes their removal. Here, we investigate whether safeguards against replication stress. Experiments were conducted in control HMOX1-deficient HEK293T cell lines....
Abstract For efficient coupling of droplet‐based microfluidics with mass spectrometry (MS), a spyhole drilled on the top microchip is used to sample passing droplets by electrostatic‐spray ionization (ESTASI) MS. The technique involves placing an electrode below chip under and applying high‐voltage pulses. Electrospray occurs directly from spyhole, droplet content analyzed MS without dilution or oil removal step. To demonstrate versatility this technique, we have successfully monitored...
The biological effect of CdTe quantum dots (QDs) on Halobacterium halobium R1 (H. R1) growth was analyzed by a microcalorimetric technique. By using TAM air eight channels microcalorimeter, the thermogenic curves H. were obtained at 37 °C. To analyze results, maximum heat power (P(m)) and rate constants (k) determined, which showed that they correlated to concentration QDs. addition caused gradual increase P(m) k low concentrations QDs, conspicuous decrease high concentrations. For...
Nicotinamide adenine dinucleotide (NAD+ ) level has been associated with various age-related diseases and its pharmacological modulation emerges as a potential approach for aging intervention. But human NAD+ landscape exhibits large heterogeneity. The lack of rapid, low-cost assays limits the establishment whole-blood baseline development personalized therapies, especially those poor responses towards conventional supplementations. Here, we developed an automated analyzer rapid measurement 5...
Abstract We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no‐wash quantification of analytes. Our approach is based on genetic fusion antibody fragments NanoLuc luciferase and SNAP‐tag, latter being labeled with synthetic fluorescent competitor antigen. Binding antigen, here drugs, by displaces tethered from disrupts resonance energy transfer (BRET) between fluorophore. The semisynthetic sensors display tunable response range...
Abstract We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The are chimeras of NanoLuc with either SNAP‐tag or HaloTag7. Labeling the self‐labeling tag a fluorophore shifts maximum that fluorophore. Luciferases tunable colors have applications as reporter genes, for construction biosensors and in bioimaging.
Abstract Nicotinamide mononucleotide (NMN) is a major precursor for NAD + metabolism with promising effects in treating - and aging-related pathologies. However, measuring live cell NMN dynamics was not possible, leaving key questions intracellular uptake regulation unanswered. Here we developed genetically encoded bioluminescent sensor to quantify subcellular cells by fusing engineered NMN-responsive binding domain fluorescent proteins from BRET pairs. The dissected the multimechanistic of...
Abstract Aberrant protein secretion is a central driver of tissue inflammation and destruction in rheumatoid arthritis (RA). While RA fibroblast-like synoviocytes (FLSs) exhibit prominent endoplasmic reticulum (ER) Golgi, the mechanism underlying their excessive not fully understood. Here, we identified deficiency de novo NAD + synthesis enzyme, quinolinate phosphoribosyltransferase (QPRT), as significant abnormality synovium. QPRT loss counterintuitively inflates trans-Golgi network (TGN)...
Abstract Mapping NAD + dynamics in live cells and human are essential for translating interventions into effective therapies. Yet genetically encoded sensors with better specificity pH-resistance still needed cost-effective monitoring of subcellular clinical . We introduce multicolor, resonance energy transfer-based that cover nano- to milli-molar concentration ranges measurement visualization. The captured the blood increase induced by NMN supplementation revealed distinct effects...