A5035036786- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Plasmonic and Surface Plasmon Research
- Colorectal Cancer Surgical Treatments
- RNA Interference and Gene Delivery
- Gold and Silver Nanoparticles Synthesis and Applications
- Enhanced Recovery After Surgery
- Cardiac, Anesthesia and Surgical Outcomes
- Hernia repair and management
- Monoclonal and Polyclonal Antibodies Research
- Advanced Proteomics Techniques and Applications
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Advanced Fluorescence Microscopy Techniques
- Alzheimer's disease research and treatments
- Microfluidic and Capillary Electrophoresis Applications
- Biliary and Gastrointestinal Fistulas
- Bacteriophages and microbial interactions
- Stoma care and complications
- Mitochondrial Function and Pathology
- Analytical Chemistry and Sensors
- Acute Myeloid Leukemia Research
- Extracellular vesicles in disease
Czech Academy of Sciences, Institute of Photonics and Electronics
2015-2025
Cornell University
2023-2024
Charles University
2018-2022
University Hospital in Motol
2019-2022
Extracellular vesicles (EVs) are preeminent carriers of biomarkers and have become the subject intense biomedical research for medical diagnostics using biosensors. To create effective EV-based immunoassays, it is imperative to develop surface chemistry approaches with optimal EV detection targeting transmembrane protein that not affected by cell-to-cell variability. Here, we developed a series immunoassays EVs derived from mouse monocyte cells plasmon resonance (SPR) We chemically...
We present a novel approach to reference-compensated label-free affinity biosensing in complex media. Unlike conventional approaches that employ surfaces with different biological functionalities the detection and reference channels produce sensor response, new (referred as single surface referencing (SSR)) uses functionalized split into channel which sample (detection channel) mixed biomolecules binding analyte thus inhibiting of (reference is introduced. This ensures (i) only captures (ii)...
In this study we examine the experimental use of staggered herringbone mixer (SHM) for signal enhancement a microfluidic surface plasmon resonance imaging (SPRi) affinity-based biosensor. We define (Emix) as ratio time-dependent slope sensor response SHM-based channel and that an unmixed channel; Emix is directly proportional to changes in sensitivity inversely limit detection (LOD). Measurements were carried out three SHM designs under wide range volumetric flow rates two analytes: high...
The multifunctional mitochondrial enzyme 17β-hydroxysteroid dehydrogenase type 10 might play a role in the development of Alzheimer disease via its high-affinity binding to amyloid β peptides and neuronal over-expression. It is suggested that cerebrospinal fluid levels enzyme, free or bound peptides, are potential specific biomarker disease. However, dysfunction seems many neurological diseases including multiple sclerosis. In this study, specificity changes relation over-expression was...
The scavenger receptor cysteine-rich (SRCR) family comprises a group of membrane-attached or secreted proteins that contain one more modules/domains structurally similar to the membrane distal domain type I macrophage receptor. Although no all-inclusive biological function has been ascribed SRCR family, some these receptors have shown recognize pathogen-associated molecular patterns (PAMP) bacteria, fungi other microbes. SSc5D is recently described soluble produced by monocytes/macrophages...
Surface plasmon resonance (SPR) sensors are known to be able detect very low surface concentrations of (bio)molecules on macroscopic areas. To explore the potential SPR biosensors achieve single-molecule detection, we have minimized read-out area (to ∼64 μm2) by employing a sensor system based spectroscopy plasmons generated diffractive structure via microscope objective and light collection through small aperture. This approach allows for decreasing number detected molecules 3 orders...
We present a portable surface plasmon resonance (SPR) sensor based on spectroscopy of plasmons special diffractive structure. The combines microfluidic cartridge incorporating the structure functionalized with DNA probes and compact SPR reader. apply to detection nucleic acids employing two most common immobilization methods: (a) biotinylated immobilized using biotin-streptavidin chemistry (b) thiolated attached directly sensor. It is demonstrated that both these methods allow detecting...
Aberrant glycosylation of glycoproteins has been linked with various pathologies. Therefore, understanding the relationship between aberrant patterns and onset progression disease is an important research goal that may provide insights into cancer diagnosis new therapy development. In this study, we use a surface plasmon resonance imaging biosensor lectin array to investigate associated oncohematological disease-myelodysplastic syndromes (MDS). particular, detected interaction lectins...
The field of chemo- and biosensors, ranging from biomedical/clinical applications to environmental food analyses, has been growing in the last two decades [...]
Abstract Myelodysplastic syndromes (MDS) are a heterogeneous group of hematological malignancies with high risk transformation to acute myeloid leukemia (AML). MDS associated posttranslational modifications proteins and variations in the protein expression levels. In this work, we present novel interactomic diagnostic method based on both array surface plasmon resonance biosensor technology, which enables monitoring protein-protein interactions label-free manner. contrast conventional...
Multifunctional mitochondrial enzyme 17β-hydroxysteroid dehydrogenase type 10 plays a role in the development of Alzheimer's disease. However, changes its expression brain or cerebrospinal fluid are not fully specific for this dementia. Our previous study revealed that complexes and amyloid β could serve as more biomarker disease than either individually when compared to autoimmune multiple sclerosis. In study, enzyme-linked immunosorbent assay surface plasmon resonance biosensor method were...