A new concept of compact biochip for surface plasmonenhanced fluorescence assays is reported.It takes advantage the amplification signal through coupling fluorophore labels with confined and strongly enhanced field intensity plasmons.In order to efficiently excite collect emitted light via plasmons on a metallic sensor surface, (reverse) Kretschmann configuration combined diffractive optical elements embedded chip surface.These include concentric relief grating imaging highly directional...

A novel nanofabrication technique based on 4-beam interference lithography is presented that enables the preparation of large macroscopic areas (>50 mm2) perfectly periodic and defect-free two-dimensional plasmonic arrays nanoparticles as small 100 nm. The a special interferometer, composed two mirrors sample with photoresist together form right-angled corner reflector. In such an incoming expanded laser beam split into four interfering beams yield pattern rectangular symmetry....

Functional polymer coatings that combine the ability to resist nonspecific fouling from complex media with high biorecognition element (BRE) immobilization capacity represent an emerging class of new functional materials for a number bioanalytical and biosensor technologies medical diagnostics, security, food safety. Here, we report on random copolymer brush surface - poly(CBMAA-ran-HPMAA) providing BRE while simultaneously exhibiting ultralow-fouling behavior in media. We demonstrate both...

Refractometric sensors utilizing surface plasmon resonance (SPR) should satisfy a series of performance metrics, bulk sensitivity, thin-film refractive-index resolution, and high-Q-factor resonance, as well practical requirements such manufacturability the ability to separate optical fluidic paths via reflection-mode sensing. While many geometries nanohole, nanoslit, nanoparticles have been employed, it is nontrivial engineer nanostructures all aforementioned requirements. We combine gold...

Abstract Spatial light modulators have become an essential tool for advanced microscopy, enabling breakthroughs in 3D, phase, and super-resolution imaging. However, continuous spatial-light modulation that is capable of capturing sub-millisecond microscopic motion without diffraction artifacts polarization dependence challenging. Here we present a photothermal spatial modulator (PT-SLM) fast phase imaging nanoscopic 3D reconstruction. The PT-SLM can generate step-like wavefront change, free...

Labeling of biological matter is a key technique enabling visualization and real-time tracking dynamic processes beyond the diffraction limit. While precision fluorescence-based localization microscopy limited by signal saturation, new generation small scattering labels (such as gold nanoparticles) have brought below size single protein. However, also most obvious handicap, they are usually significantly larger than resulting precision. We address this inconsistency demonstrate detection...

A novel method enabling rapid fabrication of 2D periodic arrays plasmonic nanoparticles across large areas is presented. This based on the interference multiple coherent beams originating from diffraction large-diameter collimated beam a transmission phase mask. Mutual orientation interfering determined by parameters used Herein, mask (periods and modulation depth) are selected to yield an pattern with high contrast narrow well-separated maxima. Finally, lithography (MBIL)-based nanomotifs...

Microtubules are cytoskeletal polymers of tubulin dimers assembled into protofilaments that constitute nanotubes undergoing periods assembly and disassembly. Static electron micrographs suggest a structural transition straight curved ones occurring at the tips disassembling microtubules. However, these transitions have never been observed process microtubule disassembly thus remains unclear. Here, label-free optical microscopy capable selective imaging transient changes tip is introduced....

Surface plasmon resonance (SPR) sensors are known to be able detect very low surface concentrations of (bio)molecules on macroscopic areas. To explore the potential SPR biosensors achieve single-molecule detection, we have minimized read-out area (to ∼64 μm2) by employing a sensor system based spectroscopy plasmons generated diffractive structure via microscope objective and light collection through small aperture. This approach allows for decreasing number detected molecules 3 orders...

Poloxamer 188 (P188), a poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) triblock copolymer, protects cell membranes against various external stresses, whereas (PEO; 8600 g/mol) homopolymer lacks protection efficacy. As part of comprehensive effort to elucidate the mechanism, we used surface plasmon resonance (SPR) obtain direct evidence binding polymers onto supported lipid bilayers. Binding kinetics and coverage P188 PEO were examined compared. Most notably, exhibited...

We report a SPR biosensor based on long-range (LR) and short-range (SR) surface plasmon (SP) modes excited simultaneously diffraction grating. Employing the LRSP SRSP in grating-coupled sensor offers several interesting features such as extended probe depth of ability to distinguish response caused by bulk refractive index changes. Prototype device wavelength interrogation SPs was developed be tested model refractometric experiment. This paper presents results theoretical analysis...

We present a portable surface plasmon resonance (SPR) sensor based on spectroscopy of plasmons special diffractive structure. The combines microfluidic cartridge incorporating the structure functionalized with DNA probes and compact SPR reader. apply to detection nucleic acids employing two most common immobilization methods: (a) biotinylated immobilized using biotin-streptavidin chemistry (b) thiolated attached directly sensor. It is demonstrated that both these methods allow detecting...

New composite layer architecture of 3D hydrogel polymer network that is loaded with mole­cularly imprinted nanoparticles (nanoMIP) reported for direct optical detection low‐molecular‐weight compounds. This attached to the metallic surface a plasmon resonance (SPR) sensor in order simultaneously serve as an waveguide and large capacity binding‐matrix target analyte. Optical spectroscopy (OWS) used label‐free readout method allowing measurement refractive index changes are associated molecular...

A label-free surface plasmon resonance biosensor method was applied to determine tetrodotoxin (TTX) in pufferfish matrixes using an antibody inhibition assay format. prevalidation study conducted demonstrate the performance characteristics, such as selectivity, LOD, LOQ, repeatability, reproducibility, and accuracy. Three participating laboratories reported standard curves buffer matrix. set of blind samples with TTX spiked into well 10% extract were analyzed. Additionally, three naturally...

This paper description Sound Source Localization problem. At present, is a developing sector in the measurement of acoustic signals. The aim this to locate source signal and evaluate angle distance between sources measuring system. With development information technology leads new researches field localization sound Recent research system that would similar as closely possible human evaluation mechanism

In the last decade surface plasmon resonance (SPR) biosensors have made great strides both in terms of technology and its applications. SPR become a central tool for study molecular interactions been widely used detection chemical biological analytes. Food analysis belongs to major areas potential applications biosensors. Therefore, numerous analytes implicated food safety (e.g. pathogens, toxins, drug residues, vitamins, hormones, contaminants, allergens) developed. This paper reviews...

This report finishes a research of the floriculture sector's recent advancements in India, notably terms key cut flower producing states, industry growth, and export potential. The primary focus is on commercially farmed loose flowers. Documentation was carried out throughout previous two decades. purpose this study to look at performance, composition exports, means transportation for share exports. India produces roughly 19 lakh Tonnes flowers 8.90 per year 3.40 hectares land, bringing...

rHIgM22 is a recombinant human monoclonal IgM designed to promote remyelination, and it currently in Phase I clinical trials patients with multiple sclerosis (MS). In animal models of demyelination, single low dose stimulates oligodendrocyte maturation, induces preserves axons, slows the decline locomotor deficits. Natural autoantibodies like typically bind antigens weak affinity. binds oligodendrocytes myelin. Because for prevalent within exclusive central nervous system (CNS) myelin, we...