A5000660636- Marine Toxins and Detection Methods
- Spectroscopy and Chemometric Analyses
- Identification and Quantification in Food
- Melamine detection and toxicity
- Advanced Chemical Sensor Technologies
- Nicotinic Acetylcholine Receptors Study
- Biosensors and Analytical Detection
- Marine Biology and Environmental Chemistry
- Advanced biosensing and bioanalysis techniques
- Mercury impact and mitigation studies
- Mass Spectrometry Techniques and Applications
- Mycobacterium research and diagnosis
- Edible Oils Quality and Analysis
- Dye analysis and toxicity
- Cultural Heritage Materials Analysis
- Microplastics and Plastic Pollution
- Effects and risks of endocrine disrupting chemicals
- Veterinary Medicine and Surgery
- Meat and Animal Product Quality
- Polyamine Metabolism and Applications
- Bacteriophages and microbial interactions
- Analytical Chemistry and Chromatography
- Analytical chemistry methods development
- Tattoo and Body Piercing Complications
- Polymer Science and PVC
Center for Food Safety and Applied Nutrition
2014-2024
United States Food and Drug Administration
2014-2024
University of Maryland, College Park
2011
Iowa State University
2007-2010
Ames National Laboratory
2007-2010
National Animal Disease Center
2007
A non-targeted detection method using near-infrared (NIR) spectroscopy combined with chemometric modeling was developed for the rapid screening of commercial milk powder (MP) products as authentic or potentially mixed known and unknown adulterants. Two benchtop FT-NIR spectrometers a handheld NIR device were evaluated model development. The performance SIMCA classification models then validated an independent test set genuine MP samples gravimetrically prepared mixtures consisting MPs spiked...
ABSTRACT A sandwich immunoassay for the rapid, low-level detection of Mycobacterium avium subsp. paratuberculosis has been developed. M. is causative agent Johne's disease in cattle, and one major obstacles controlling spread this inability to rapidly detect small amounts bacteria or other diagnostic markers shed during subclinical stage infection. This paper details development performance an assay sonicated lysate that based on surface-enhanced Raman scattering (SERS). There are two key...
The United States Pharmacopeial Convention has led an international collaborative project to develop a toolbox of screening methods and reference standards for the detection milk powder adulteration. During development adulterated standards, blending used combine melamine had unanticipated strong effects on near-infrared (NIR) spectrum melamine. prominent absorbance band at 1468 nm was retained when it dry-blended with skim but disappeared in wet-blended mixtures, where spray-dried samples...
The industrial chemical melamine was used in 2007 and 2008 to raise the apparent protein content pet feed watered down milk, respectively. Because humans may be exposed via several different routes into human diet as well deliberate contamination, this study designed characterize effect of high dose or cyanuric acid oral exposure on pregnant animal developing fetus, including placental transfer. Clear rectangular crystals formed following a single triazine which is morphology from golden...
Tetrodotoxin (TTX) is a low molecular weight neurotoxin found in number of animal species, including pufferfish. One emerging method for TTX detection employs surface plasmon resonance (SPR) immunosensors. SPR, an optical technique that allows label-free, real-time, multiplexed analysis, can have limits rival many the conventional transduction methods. Preliminary SPR approaches were successful, yet suffered from throughput and used noncommercial instrumentation. To advance this broader use,...
Tetrodotoxin (TTX), a small molecular weight neurotoxin, is responsible for poisoning events that traditionally occur from consumption of contaminated puffer fish. Recent studies have shown growing number foods with TTX and larger waters associated countries where the toxin may occur. The apparent expanding prevalence supports need screening assays can be used to detect potentially harmful food. In past few years, surface plasmon resonance (SPR) biosensors been developed rapid, robust...
Raman spectroscopy in combination with chemometrics was explored as a rapid, non-targeted screening method for the detection of milk powder (MP) adulteration using melamine an example contaminant. and unsupervised pattern-recognition method, principal component analysis (PCA), allowed differentiation authentic MPs from adulterated ones at concentrations > 1.0% dry-blended (DB) samples 0.30% wet-blended (WB) ones. Soft independent modelling class analogy (SIMCA), supervised also used to...
As a result of the increase in practice tattooing, US Food and Drug Administration has identified need for improved analytical methods to detect pigments potential impurities inks. Raman spectroscopy allows nondestructive identification compounds is commonly used art, archaeology, forensics; however, technique only limitedly been applied tattoo pigments. In this study, approximately 30 inorganic, organometallic, organic were evaluated with by using 532, 633, 780‐nm lasers. Individual...
The etiological agent of Johne's disease is Mycobacterium avium subsp. paratuberculosis. Controlling the spread this hindered by lack sensitive, selective, and rapid detection methods for M. By using a recently optimized sandwich immunoassay (B. J. Yakes, R. Lipert, P. Bannantine, D. Porter, Clin. Vaccine Immunol. 15:227-234, 2008), which incorporates new monoclonal antibody selective capture labeling paratuberculosis surface-enhanced Raman scattering sensitive readout, limits approximately...
There is a need to develop rapid tools screen milk products for economically motivated adulteration. An understanding of the physiochemical variability within skim powder (SMP) and non-fat dry (NFDM) key establishing natural differences these commodities prior development non-targeted detection methods. This study explored sources variance in 71 commercial SMP NFDM samples using Raman spectroscopy principal component analysis (PCA) characterised largest number powders acquired from broad...
Abstract This study evaluated an electronic‐nose (e‐nose) sensor in combination with support vector machine (SVM) modeling for predicting the decomposition state of four types fish fillets: mahi‐mahi, croaker, red snapper, and weakfish. The National Seafood Sensory Expert scored fillets were thawed, 10‐g portions weighed into glass jars which then sealed, held at approximately 30°C to allow volatile components be trapped available analysis. measurement sample vial headspace was performed...
Tetrodotoxin (TTX) is a low molecular weight (~319 Da) neurotoxin found in number of animal species, including pufferfish. Protection from toxin tainted food stuffs requires rapid, sensitive, and specific diagnostic tests. An emerging technique for the detection both proteins nucleic acids Fluidic Force Discrimination (FFD) assays. This simple rapid method typically uses sandwich immunoassay format labeled with micrometer-diameter beads has novel capability removing nonspecifically attached...