A5086487044- Marine Toxins and Detection Methods
- Nicotinic Acetylcholine Receptors Study
- Marine Biology and Environmental Chemistry
- Toxin Mechanisms and Immunotoxins
- Forensic Toxicology and Drug Analysis
- Transgenic Plants and Applications
- Environmental Toxicology and Ecotoxicology
- Ion channel regulation and function
- Bacillus and Francisella bacterial research
- Botulinum Toxin and Related Neurological Disorders
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Marine and coastal ecosystems
- Plant-based Medicinal Research
- Environmental Chemistry and Analysis
- Plant tissue culture and regeneration
- bioluminescence and chemiluminescence research
- Neuroscience of respiration and sleep
- Clostridium difficile and Clostridium perfringens research
- Protist diversity and phylogeny
- Diphtheria, Corynebacterium, and Tetanus
- Chemical synthesis and alkaloids
- Identification and Quantification in Food
- Yersinia bacterium, plague, ectoparasites research
- Herbal Medicine Research Studies
United States Army Medical Research Institute of Infectious Diseases
2011-2024
Pacific Northwest National Laboratory
2017
United States Department of the Army
1995-2015
Panthera Biopartners
2009
University of Trieste
2009
Centers for Disease Control and Prevention
2001
National Center for Environmental Health
2001
Universal Sensors (United States)
1993
University of Miami
1984
The polyether lipid-soluble toxins isolated from the marine dinoflagellate Ptychodiscus brevis (formerly Gymnodinium breve) have been determined to bind a unique site associated with rat brain synaptosomes. Using [3H]brevetoxin PbTx-3 as specific probe, binding was at 4 degrees in synaptosomes using rapid centrifugation technique. Rosenthal analysis yields KD of 2.9 nM and Bmax 6.8 pmol toxin/mg protein. Labeled probe can be displaced by unlabeled PbTx-3, PbTx-2, or synthetic (reduced...
Marine toxins appear to be increasing in many areas of the world. An emerging problem Mediterranean Sea is represented by palytoxin (PlTX), one most potent marine toxins, frequently detected seafood. Due high potential for human toxicity PlTX, there a strong and urgent need sensitive methods toward its detection quantification. We have developed an ultrasensitive electrochemiluminescence-based sensor taking advantage specificity provided anti-PlTX antibodies, good conductive properties...
Following four outbreaks of paralytic shellfish poisoning on Kodiak Island, Alaska, during 1994, medical records ill persons were reviewed and interviews conducted. Urine serum specimens analyzed at three independent laboratories using different saxitoxin binding assays. High-performance liquid chromatography was used to determine the presence specific toxin congeners. Among 11 persons, required mechanical ventilation one died. Mean peak systolic diastolic blood pressure measurements 172...
This paper reports on the analysis of toxin content from Palythoa tuberculosa and toxica samples collected off Hawaiian coast. Our work, based in-depth high-resolution liquid chromatography−mass spectrometry along with extensive NMR study, led us to structurally characterize 42-hydroxy-palytoxin, a new palytoxin congener. itself appeared be major components toxic extract P. sample, while 42-hydroxy-palytoxin was proven by far main derivative in toxica. Functional studies this palytoxin-like...
In October of 1996, a Gymnodinium breve bloom occurred in shellfish harvesting waters Alabama, Mississippi and Louisiana, Gulf Mexico, USA. Bloom densities reached 5.6x10(5) cells liter(-1) residence at sampling stations ranged from 3 to 28 days. Brevetoxin-2 dominated G. toxin profiles seawater extracts. Shellfish toxicity, assessed by mouse bioassay, exceeded the guidance level for up 75 days after had dissipated. Cytotoxicity assays bioassays showed similar temporal patterns but two...
In October of 1996, a Gymnodinium breve bloom occurred in shellfish harvesting waters Alabama, Mississippi and Louisiana, Gulf Mexico, USA. Bloom densities reached 5.6x10(5) cells liter(-1) residence at sampling stations ranged from 3 to 28 days. Brevetoxin-2 dominated G. toxin profiles seawater extracts. Shellfish toxicity, assessed by mouse bioassay, exceeded the guidance level for up 75 days after had dissipated. Cytotoxicity assays bioassays showed similar temporal patterns but two...
Abstract Brevetoxins are a family of ladder-frame polyether toxins produced during blooms the marine dinoflagellate Karenia brevis. Consumption fish exposed to K. brevis can lead development neurotoxic shellfish poisoning. The toxic effects brevetoxins due activation voltage-sensitive sodium channels (VSSCs) in cell membranes. Binding has historically been measured using radioligand competition assay that is fraught with difficulty. In thisstudy, we developed novel fluorescence-based binding...
This study provides the first evaluation of cytotoxic effects recently identified palytoxin (PLTX) analog, ovatoxin-a (OVTX-a), major toxin produced by Ostreopsis cf. ovata in Mediterranean Sea. Its increasing detection during blooms and seafood highlights need to characterize its toxic set up appropriate methods. OVTX-a is about 100 fold less potent than PLTX reducing HaCaT cells viability (EC50 = 1.1 × 10(-9) M vs 1.8 10(-11) M, MTT test) agreement with a reduced binding affinity (Kd 1.2...
Ostreopsis cf. ovata, a benthic dinoflagellate often blooming along the Mediterranean coasts, has been associated with toxic events ranging from dyspnea to mild dermatitis. In late September 2009, an ovata bloom occurred in Gulf of Trieste (Northern Adriatic Sea; Italy), causing pruritus and dermatitis beachgoers. An integrated study was initiated characterize cells by light confocal microscopy, PCR techniques, immunocytochemistry, high resolution liquid chromatography–mass spectrometry (HR...
Palytoxins are potent marine biotoxins that have recently become endemic to the Mediterranean Sea, and becoming more frequently associated with seafood. Due their high toxicity, suitable methods quantify palytoxins needed. Thus, we developed an indirect sandwich ELISA for palytoxin 42-hydroxy-palytoxin. An intralaboratory study demonstrated sensitivity (limit of detection, LOD = 1.1 ng/mL; limit quantitation, LOQ 2.2 ng/mL), accuracy (bias 2.1%), repeatability (RSDr 6% 9% intra- interassay...