A5016878952- Single-cell and spatial transcriptomics
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Service-Oriented Architecture and Web Services
- Advanced Computational Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Prenatal Screening and Diagnostics
- Distributed and Parallel Computing Systems
- Neuroinflammation and Neurodegeneration Mechanisms
- Axon Guidance and Neuronal Signaling
- Renal and related cancers
- Pluripotent Stem Cells Research
- Immune cells in cancer
- MicroRNA in disease regulation
- Retinal Development and Disorders
- Zebrafish Biomedical Research Applications
- Intracerebral and Subarachnoid Hemorrhage Research
- Vagus Nerve Stimulation Research
- Extracellular vesicles in disease
- Cancer, Stress, Anesthesia, and Immune Response
- Eosinophilic Esophagitis
Inserm
2020-2023
Centre d’Immunologie de Marseille-Luminy
2020-2023
Centre National de la Recherche Scientifique
2020-2023
Aix-Marseille Université
2020-2022
Max Planck Institute for Molecular Biomedicine
2021
Weatherford College
2021
Jena University Hospital
2021
University of Münster
2021
Cincinnati Children's Hospital Medical Center
2014-2015
Significance The retina is subject to a variety of insults that lead degeneration one or more types neurons and ultimate visual impairment blindness. Although the retinas nonmammalian vertebrates can regenerate new after injury, mammalian largely lack this potential. We have tested whether expression proneural transcription factor Ascl1 may be key difference between fish mouse by targeting cells provide retinal progenitors in mature retina, Müller glia. Our results show at least differences...
The activity of adult stem cells is regulated by signals emanating from the surrounding tissue. Many niche have been identified, but it unclear how they influence choice to remain quiescent or divide. Here we show that when hippocampus receive activating signals, first induce expression transcription factor Ascl1 and only subsequently exit quiescence. Moreover, lowering reduces proliferation rate hippocampal cells, inactivating blocks quiescence completely, rendering them unresponsive...
Abstract Neuronal nerve processes in the tumor microenvironment were highlighted recently. However, origin of intra-tumoral nerves remains poorly known, part because technical difficulties tracing fibers via conventional histological preparations. Here, we employ three-dimensional (3D) imaging cleared tissues for a comprehensive analysis sympathetic innervation murine model pancreatic ductal adenocarcinoma (PDAC). Our results support two independent, but coexisting, mechanisms: passive...
Abstract Perivascular macrophages (pvMs) are associated with cerebral vasculature and mediate brain drainage immune regulation. Here, using reporter mouse models, whole section immunofluorescence, flow cytometry, single cell RNA sequencing, besides the Lyve1 + F4/80 CD206 CX3CR1 pvMs, we identify a – pvM population that shares phagocytic functions location. Furthermore, parenchyma mostly hosts MHCII pvMs low to intermediate CD45 expression. Using double Cx3cr1 GFP x Cx3cr1-Cre ; Rosa tdT...
The innate lymphoid cell (ILC) family consists of NK cells, ILC type 1, 2, 3 and tissue inducer cells. They have been shown to play important roles in homeostasis immune responses are generally considered resident. Not much is known about the presence members within central nervous system whether they resident this organ too. Therefore, we studied all after ischemic brain insult.We used photothrombotic lesion method induce lesions mouse brain. Using whole-mount immunofluorescence imaging,...
Highlights•LTi progenitors originate from early embryonic hemogenic endothelium, excluding yolk sac•The fetal liver harbors proliferating LTi precursor populations•LTi maturation occurs in the periphery into two LTi4 populations•Embryonic are replaced by HSC-derived cells adultsSummaryDuring embryogenesis, lymphoid tissue inducer (LTi) essential for lymph node organogenesis. These part of innate cell (ILC) family. Although their earliest hematopoietic origin is unclear, other immune have...
Abstract Innate lymphoid cells (ILCs) have a function in homeostasis and immune responses, but their role ischemic brain insult is unknown. Here, we report that ILCs are not resident within the mouse parenchyma during steady-state conditions, attracted after stroke. Specifically, identified NK cells, ILC1s, ILC2s ILC3s lesion, highest influx being observed for ILC1s. We further show Cxcl12 expressed at blood-brain barrier essential NKp46 + to migrate toward lesion. Complementary, Cxcr4...
Abstract Perivascular macrophages (pvM) are closely associated with cerebral vasculature and play an essential role in drainage of the brain regulation immune response. Here, using reporter mouse models immunofluorescence on sections whole brain, flow cytometry single cell sequencing, we identify a Lyve1+ perivascular population lacking classical macrophage markers such as CD45 Cx3cr1. We named new non-conventional negative pvM2. These cells have similar location, morphology phagocytic...