A5025436080- MicroRNA in disease regulation
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Zebrafish Biomedical Research Applications
- Neuroscience and Neuropharmacology Research
- Circular RNAs in diseases
- Developmental Biology and Gene Regulation
- Genomics, phytochemicals, and oxidative stress
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- GDF15 and Related Biomarkers
- Advanced Memory and Neural Computing
- RNA modifications and cancer
- Diet and metabolism studies
- Neuroscience and Neural Engineering
- RNA and protein synthesis mechanisms
- Retinoids in leukemia and cellular processes
University of California, San Diego
2017-2023
Pfizer (United States)
2023
University of Washington
2015-2020
Significance The retina is subject to a variety of insults that lead degeneration one or more types neurons and ultimate visual impairment blindness. Although the retinas nonmammalian vertebrates can regenerate new after injury, mammalian largely lack this potential. We have tested whether expression proneural transcription factor Ascl1 may be key difference between fish mouse by targeting cells provide retinal progenitors in mature retina, Müller glia. Our results show at least differences...
Argonaute (AGO) proteins partner with microRNAs (miRNAs) to target specific genes for post-transcriptional regulation. During larval development in Caenorhabditis elegans, Argonaute-Like Gene 1 (ALG-1) is the primary mediator of miRNA pathway, while related ALG-2 protein largely dispensable. Here we show that adult C. elegans these AGOs are differentially expressed and, surprisingly, work opposition each other; alg-1 promotes longevity, whereas alg-2 restricts lifespan. Transcriptional...
Abstract Diseases and damage to the retina lead losses in retinal neurons eventual visual impairment. Although mammalian has no inherent regenerative capabilities, fish have robust regeneration from Müller glia (MG). Recently, we shown that driving expression of Ascl1 adult mouse MG stimulates neural regeneration. The observed is limited variety can be derived MG; -expressing primarily generate bipolar cells. To better understand limits MG-based retinas, used ATAC- RNA-seq compare newborn...
The primary glial cells in the retina, Müller glia, differentiate from retinal progenitors first postnatal week. CNTF/LIF/STAT3 signaling has been shown to promote their differentiation; however, another key differentiation signal, BMP, not examined during this period of differentiation. In course our analysis BMP pathway, we observed a transient wave Smad1/5/8 inner nuclear layer at end week, day (P) 5 P9, after neurogenesis. To determine function wave, blocked vitro or vivo, using either...
Abstract MicroRNAs (miRNAs) regulate gene expression by base-pairing to target sequences in messenger RNAs (mRNAs) and recruiting factors that induce translational repression mRNA decay. In animals, nucleotides 2-8 at the 5’ end of miRNA, called seed region, are often necessary sometimes sufficient for functional interactions. MiRNAs contain identical grouped into families where individual members have potential share targets act redundantly. A rare exception seemed be miR-238/239ab family...
MicroRNAs (miRNAs) regulate gene expression by base-pairing to target sequences in messenger RNAs (mRNAs) and recruiting factors that induce translational repression mRNA decay. In animals, nucleotides 2–8 at the 5’ end of miRNA, called seed region, are often necessary sometimes sufficient for functional interactions. MiRNAs contain identical grouped into families where individual members have potential share targets act redundantly. A rare exception seemed be miR-238/239ab family...
Abstract Dravet syndrome is a developmental and epileptic encephalopathy characterized by seizures, behavioral abnormalities, deficits, elevated risk of sudden unexpected death in epilepsy (SUDEP). Most patient cases are caused de novo loss-of-function mutations the gene SCN1A , causing haploinsufficiency alpha subunit voltage-gated sodium channel Na V 1.1. Within brain, 1.1 primarily localized to axons inhibitory neurons, decreased function hypothesized reduce GABAergic neurotransmission...
Abstract Diseases and damage to the retina lead losses in retinal neurons eventual visual impairment. Although mammalian has no inherent regenerative capabilities, fish have robust regeneration from Müller glia (MG). Recently, we shown that driving expression of Ascl1 adult mouse MG stimulates neurogenesis similar regeneration. The observed is limited variety can be derived MG; Ascl1-expressing primarily generate bipolar cells. To better understand limits MG-based retinas, used ATAC- RNA-seq...