A5036425727- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- Nanoplatforms for cancer theranostics
- Gastric Cancer Management and Outcomes
- Glycosylation and Glycoproteins Research
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- bioluminescence and chemiluminescence research
- Surgical Simulation and Training
- MicroRNA in disease regulation
- Retinoids in leukemia and cellular processes
- Cardiac, Anesthesia and Surgical Outcomes
- Animal Genetics and Reproduction
- Monoclonal and Polyclonal Antibodies Research
- Cancer Mechanisms and Therapy
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer-related molecular mechanisms research
- Zebrafish Biomedical Research Applications
- Ferroptosis and cancer prognosis
- Viral Infectious Diseases and Gene Expression in Insects
- Colorectal Cancer Screening and Detection
University of California, San Diego
2023-2025
VA San Diego Healthcare System
2023-2025
University of Washington
2013-2020
Significance The retina is subject to a variety of insults that lead degeneration one or more types neurons and ultimate visual impairment blindness. Although the retinas nonmammalian vertebrates can regenerate new after injury, mammalian largely lack this potential. We have tested whether expression proneural transcription factor Ascl1 may be key difference between fish mouse by targeting cells provide retinal progenitors in mature retina, Müller glia. Our results show at least differences...
Non-mammalian vertebrates have a robust ability to regenerate injured retinal neurons from Müller glia (MG) that activate the gene encoding proneural factor Achaete-scute homolog 1 (Ascl1; also known as Mash1 in mammals) and de-differentiate into progenitor cells. By contrast, mammalian MG limited regenerative response fail upregulate Ascl1 after injury. To test whether ASCL1 could restore neurogenic potential MG, we overexpressed dissociated mouse cultures intact explants. ASCL1-infected...
Claudins (CLDN1–CLDN24) are a family of tight junction proteins whose dysregulation has been implicated in tumorigeneses many cancer types. In colorectal (CRC), CLDN1, CLDN2, CLDN4, and CLDN18 have shown to either be upregulated or aberrantly expressed. the normal colon, CLDN1 CLDN3–7 Although few claudins, such as CLDN6 CLDN7, expressed CRC their levels reduced compared colon. The present review outlines expression profiles claudin those that potential biomarkers for prognostication.
Abstract Diseases and damage to the retina lead losses in retinal neurons eventual visual impairment. Although mammalian has no inherent regenerative capabilities, fish have robust regeneration from Müller glia (MG). Recently, we shown that driving expression of Ascl1 adult mouse MG stimulates neural regeneration. The observed is limited variety can be derived MG; -expressing primarily generate bipolar cells. To better understand limits MG-based retinas, used ATAC- RNA-seq compare newborn...
Abstract Background Gastric cancer poses a major diagnostic and therapeutic challenge as surgical resection provides the only opportunity for cure. Specific labeling of gastric could distinguish resectable nonresectable disease facilitate an R0 resection, which improve survival. Methods Two patient-derived lines, KG8 KG10 , were established from specimens two patients who underwent gastrectomy adenocarcinoma. Harvested tumor fragments implanted into greater curvature stomach to establish...
The primary glial cells in the retina, Müller glia, differentiate from retinal progenitors first postnatal week. CNTF/LIF/STAT3 signaling has been shown to promote their differentiation; however, another key differentiation signal, BMP, not examined during this period of differentiation. In course our analysis BMP pathway, we observed a transient wave Smad1/5/8 inner nuclear layer at end week, day (P) 5 P9, after neurogenesis. To determine function wave, blocked vitro or vivo, using either...
The present study aimed to validate the accuracy of a tumor-specific antibody target liver metastases colorectal cancer.
Background/Objectives: Pancreatic cancer is the third leading cause of death related to cancer. The only possible cure presently complete surgical resection; however, this limited by difficulty in clearly defining tumor margins. Enhancement visualization pancreatic ductal adenocarcinoma (PDAC) margins using near-infrared dye-conjugated tumor-specific antibodies was pioneered anti-CEA, anti-CA19.9, and anti-MUC5AC orthotopic mouse models Recently, an antibody Mucin 4 (MUC4) conjugated a...
Abstract Introduction: Gastric cancer has a global incidence of ~1 million cases per year. Currently, complete surgical resection is the only opportunity for cure patients. Bright labeling tumor margins with specific markers could improve outcomes. This report demonstrates advantages targeting gastric humanized anti-CEA and TAG-72 antibodies in mouse models. Methods: A cell line human cancer, MKN45, patient-derived tumor, KG8, were used to create xenograft To establish orthotopic models...
Accurately identifying metastatic disease is critical to directing the appropriate treatment in pancreatic cancer. Mucin 5AC overexpressed cancer but absent normal pancreas tissue. The present proof-of-concept study demonstrates efficacy of an anti-mucin antibody conjugated IR800 dye (MUC5AC-IR800) preferentially label a liver metastasis (Panc Met) unique patient-derived orthotopic xenograft (PDOX) model. In models, mean tumor background ratio was 1.787 (SD ± 0.336), and immunohistochemistry...
Abstract Diseases and damage to the retina lead losses in retinal neurons eventual visual impairment. Although mammalian has no inherent regenerative capabilities, fish have robust regeneration from Müller glia (MG). Recently, we shown that driving expression of Ascl1 adult mouse MG stimulates neurogenesis similar regeneration. The observed is limited variety can be derived MG; Ascl1-expressing primarily generate bipolar cells. To better understand limits MG-based retinas, used ATAC- RNA-seq...