A5060354018- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- Cancer Cells and Metastasis
- Photodynamic Therapy Research Studies
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Immune cells in cancer
- Gastric Cancer Management and Outcomes
- Glycosylation and Glycoproteins Research
- Ultrasound and Hyperthermia Applications
- Monoclonal and Polyclonal Antibodies Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Gastrointestinal Tumor Research and Treatment
- Mycobacterium research and diagnosis
- Metastasis and carcinoma case studies
- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Virus-based gene therapy research
- 3D Printing in Biomedical Research
- Photoacoustic and Ultrasonic Imaging
- Cancer Immunotherapy and Biomarkers
- Colorectal Cancer Surgical Treatments
VA San Diego Healthcare System
2018-2025
University of California, San Diego
2017-2025
University of Essex
2016-2017
Abstract Background Gastric cancer poses a major diagnostic and therapeutic challenge as surgical resection provides the only opportunity for cure. Specific labeling of gastric could distinguish resectable nonresectable disease facilitate an R0 resection, which improve survival. Methods Two patient-derived lines, KG8 KG10 , were established from specimens two patients who underwent gastrectomy adenocarcinoma. Harvested tumor fragments implanted into greater curvature stomach to establish...
// Thinzar M. Lwin 1 , Kentaro Miyake 1, 2, 3 Takashi Murakami Jonathan C. DeLong Siamak Amirfakhri 4 Filemoni Sang Nam Yoon 2 Paul J. Yazaki 5 John E. Shivley Brian Datnow 6 Bryan Clary Robert Hoffman and Michael Bouvet Department of Surgery, University California San Diego, CA, USA AntiCancer, Inc., Gastroenterological Yokohama City University, Graduate School Medicine, Yokohama, Japan VA Diego Healthcare System, Molecular Imaging Therapy, Hope, Duarte, Pathology, Correspondence to:...
Background Photoimmunotherapy (PIT) employs the use of a near-infrared (NIR) laser to activate an antibody conjugated NIR-activatable dye induce cancer cell death. PIT has shown be effective in number studies, however, there are no data on its colorectal orthotopic model. Methods Humanized anti-CEA (M5A) was IRDye700DX (M5A-700). validated vitro with colon cell-line, using intensity either 4 J/cm2, 8 or 16 J/cm2. Orthotopic mouse models were established by surgical implantation LS174T tumor...
Overexpression of mucin-5AC (MUC5AC) makes it a targetable biomarker in pancreatic cancer. The present study evaluated tumor targeting with MUC5AC antibody conjugated to near-infrared dye patient-derived orthotopic xenograft (PDOX) mouse model.MUC5AC monoclonal was the IRDye800CW synthesize MUC5AC-IR800. PDOX models were established by implanting high-MUC5AC-expressing on pancreas nude mice. After 4 weeks growth, mice imaged after receiving MUC5AC-IR800 (75 μg) intravenously.In models,...
HopQ is an outer-membrane protein of Helicobacter pylori that binds to human carcinoembryonic antigen-related cell-adhesion molecules (CEACAMs) with high specificity. We aimed investigate fluorescence targeting CEACAM-expressing colorectal tumors in patient-derived orthotopic xenograft (PDOX) models fluorescently labeled recombinant (rHopQ). Western blotting, flow cytometry and ELISA were performed determine the efficiency rHopQ binding CEACAMs. was conjugated IR800DyeCW (rHopQ-IR800). Nude...
Fluorescence imaging has been shown to improve intra-operative detection of liver metastasis. The present study aimed determine whether humanized anti-TAG-72 antibody (huCC49) conjugated a near-infrared dye provides selective labeling colorectal-cancer metastasis in orthotopic mouse models.Humanized was IRDye800CW (huCC49-IR800). Orthotopic liver-metastasis nude-mouse models (n=5) were established with the human colon-cancer LS174T cell-line. Three weeks later, mice administered huCC49-IR800...
Breast cancer is the most common neoplasm in women and frequent cause of death those between 35 55 years age. All multicellular organisms have an innate immune system, whereas adaptive or 'acquired' system restricted to vertebrates. This study focused on effect conditioned medium isolated from cultured breast cells NB4 neutrophil-like cells.In current were incubated with MCF-7 cell-conditioned medium. After 6 h incubation intracellular receptor TLR2, was analyzed.The results revealed that...
Background/Aim: Near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) is used to visualize colon-cancer liver metastases (CCLM) during surgery. The present study aimed use ICG small CCLM in an orthotopic mouse model. Materials and Methods: A 3-mm fragment of HT29 human colon cancer was implanted the 5 athymic nude mice (nu/nu). Pearl Trilogy Small Animal Fluorescence Imaging system 24 h after intravenous (IV) injection 0.025 mg (0.25 mg/ml) ICG. Results: In four five mice,...
Background/Aim: During surgical resection of gastroesophageal-junction (GEJ) adenocarcinoma, the margin status is often difficult to visualize resulting in high recurrence rates. The aim present study was develop a labelling technique that would allow improved visualization GEJ tumor margins for surgeons reduce rates patient-like model. Materials and Methods: A patient obtained from an endoscopic biopsy implanted subcutaneously nude mouse. patient-derived orthotopic xenograft (PDOX) model...
Abstract Background Pancreatic cancer is a recalcitrant disease in which R0 resection often not achieved owing to difficulty visualization of the tumor margins and proximity adjacent vessels. To improve outcomes, we have developed fluorescence-guided surgery (FGS) photoimmunotherapy (PIT) using fluorescent tumor-specific antibody. Methods Nude mice received surgical orthotopic implantation (SOI) human pancreatic cell line BxPC-3 expressing green protein. An anti-carcinoembryonic...
Background/Objectives: Pancreatic cancer is the third leading cause of death related to cancer. The only possible cure presently complete surgical resection; however, this limited by difficulty in clearly defining tumor margins. Enhancement visualization pancreatic ductal adenocarcinoma (PDAC) margins using near-infrared dye-conjugated tumor-specific antibodies was pioneered anti-CEA, anti-CA19.9, and anti-MUC5AC orthotopic mouse models Recently, an antibody Mucin 4 (MUC4) conjugated a...
Accurately identifying metastatic disease is critical to directing the appropriate treatment in pancreatic cancer. Mucin 5AC overexpressed cancer but absent normal pancreas tissue. The present proof-of-concept study demonstrates efficacy of an anti-mucin antibody conjugated IR800 dye (MUC5AC-IR800) preferentially label a liver metastasis (Panc Met) unique patient-derived orthotopic xenograft (PDOX) model. In models, mean tumor background ratio was 1.787 (SD ± 0.336), and immunohistochemistry...
Monocytes are important regulators for the maintenance of homeostasis in innate and adaptive immune system have been reported to play role cancer progression. CD47-SIRPα recognition is a coinhibitory signal inhibit phagocytosis monocytes macrophages has well-known as "Don't eat me" signal. By using an approach integrated sensing activating proteins (iSNAPs), we rewired axis create iSNAP-M which activates pathways engineered human (iSNAP-MC). The mRNA expression levels monocyte/macrophage...
INTRODUCTION: While immunotherapy through anti-PD1 checkpoint inhibition has demonstrated impressive clinical efficacy against a number of tumor types, its application been ineffective glioblastomas METHODS: We wished to determine whether high intensity focused ultrasound (HIFU) augment the glioblastomas. HIFU can be administered low-duty cycle induce subcellular fragmentation without causing temperature elevation (termed mechanical HIFU). Alternatively, delivered high-duty foci and thermal...
Despite aggressive treatment, most glioblastoma patients succumb to the disease within two years of diagnosis. The molecular basis for rare who exhibit “exceptional response” and survive beyond expectation remains poorly understood. Using three independent patient cohorts, we showed that decreased expression an inflammation gene signature consistently associated with response”. Integrated cell-based analysis suggests glioblastoma-associated microglia as principle mediator this signature....