A5018457159- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Neuroscience and Neural Engineering
- MicroRNA in disease regulation
- Zebrafish Biomedical Research Applications
- Nicotinic Acetylcholine Receptors Study
- Epigenetics and DNA Methylation
- Neurotransmitter Receptor Influence on Behavior
- Congenital heart defects research
- Neuroscience and Neuropharmacology Research
- Neurogenetic and Muscular Disorders Research
- CRISPR and Genetic Engineering
- PI3K/AKT/mTOR signaling in cancer
- RNA Interference and Gene Delivery
- Modular Robots and Swarm Intelligence
- 3D Printing in Biomedical Research
- Psychedelics and Drug Studies
- Mechanisms of cancer metastasis
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Development and Disorders
Stanford University
2017-2024
Organogenesis (United States)
2020-2024
Emory University
2024
Neuroscience Institute
2023
Neurosciences Institute
2020-2021
The Francis Crick Institute
2013-2017
Yale University
2010
Abstract Self-organizing neural organoids represent a promising in vitro platform with which to model human development and disease 1–5 . However, lack the connectivity that exists vivo, limits maturation makes integration other circuits control behaviour impossible. Here we show stem cell-derived cortical transplanted into somatosensory cortex of newborn athymic rats develop mature cell types integrate sensory motivation-related circuits. MRI reveals post-transplantation organoid growth...
Sending neural stem cells back to the garage In brain's hippocampus, which modulates memories and emotions, generate new neurons, even during adulthood. How many neurons are generated, when, follows from balance between quiescence proliferation in pool of cells. Urbán et al. asked what signals send proliferating into a quiescent state. They found that key transcription factor promotes cellular was degraded through ubiquitinylation system. This molecular interaction regulated return resting...
Organoids recapitulate brain development Gene expression changes and their control by accessible chromatin in the human during is of great interest but limited accessibility. Trevino et al. avoided this problem developing three-dimensional organoid models forebrain examining accessibility gene at single-cell level. From analysis, they matched developmental profiles between fetal samples, identified transcription factor binding profiles, predicted how factors are linked to cortical...
The majority of neural stem cells (NSCs) in the adult brain are quiescent, and this fraction increases with aging. Although signaling pathways that promote NSC quiescence have been identified, transcriptional mechanisms involved mostly unknown, largely due to lack a cell culture model. In study, we first demonstrate cultures (NS cells) exposed BMP4 acquire cellular characteristics quiescent cells. We then use epigenomic profiling identify enhancers associated NS state. Motif enrichment...
The activity of adult stem cells is regulated by signals emanating from the surrounding tissue. Many niche have been identified, but it unclear how they influence choice to remain quiescent or divide. Here we show that when hippocampus receive activating signals, first induce expression transcription factor Ascl1 and only subsequently exit quiescence. Moreover, lowering reduces proliferation rate hippocampal cells, inactivating blocks quiescence completely, rendering them unresponsive...
Transcription factors of the nuclear factor one (NFI) family play a pivotal role in development nervous system. One member, NFIX, regulates neocortex, hippocampus, and cerebellum. Postnatal Nfix−/− mice also display abnormalities within subventricular zone (SVZ) lining lateral ventricles, region brain comprising neurogenic niche that provides ongoing neurogenesis throughout life. Specifically, exhibit more PAX6-expressing progenitor cells SVZ. However, mechanism underlying this phenotype...
Abstract Abnormalities in crosstalk between the thalamus and cerebral cortex are thought to lead severe neuropsychiatric disorders, such as epilepsy psychotic disorders. Pathogenic variants CACNA1G gene, which encodes α1G subunit of thalamus-enriched T-type voltage-gated calcium channel CaV3.1, associated with absence seizures, intellectual disability, schizophrenia, but cellular circuit level consequences these genetic humans remain unknown. Here, we developed an vitro human assembloid...
Background: The costs associated with alcohol abuse are staggering, therefore much effort has been put into developing new pharmacologic strategies to decrease abuse. Recently, the nicotinic acetylcholine receptor (nAChR) partial agonist varenicline shown ethanol consumption in both humans and animal models. Methods: We examined effects of on ataxic sedative‐hypnotic ethanol. First, was administered prior placement a locomotor activity chamber determine whether influenced baseline activity....
ABSTRACT Genetic perturbations of cerebral cortical development can lead to neurodevelopmental disease, including autism spectrum disorder (ASD). To identify genomic regions crucial corticogenesis, we mapped the activity gene-regulatory elements generating a single-cell atlas gene expression and chromatin accessibility both independently jointly. This revealed waves regulation by key transcription factors (TFs) across nearly continuous differentiation trajectory into glutamatergic neurons,...
Abstract Understanding spinal cord generation and assembly is essential to elucidate how motor behavior controlled disorders arise. The cellular landscape of the human remains, however, insufficiently explored. Here, we profiled midgestation with single cell-resolution discovered, even at this fetal stage, remarkable heterogeneity across within cell types. Glia displayed diversity related positional identity along dorso-ventral rostro-caudal axes, while astrocytes specialized transcriptional...