A5068751619- Reproductive biology and impacts on aquatic species
- Neuropeptides and Animal Physiology
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Hypothalamic control of reproductive hormones
- Neurogenesis and neuroplasticity mechanisms
- Proteoglycans and glycosaminoglycans research
- Ion channel regulation and function
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Congenital heart defects research
- Neuroscience of respiration and sleep
- RNA Research and Splicing
- Sperm and Testicular Function
- Genomics and Chromatin Dynamics
- Neonatal and fetal brain pathology
- Mitochondrial Function and Pathology
- Innovation and Socioeconomic Development
- Tissue Engineering and Regenerative Medicine
- Traumatic Brain Injury and Neurovascular Disturbances
- Retinal Development and Disorders
- Neonatal Respiratory Health Research
Hebrew University of Jerusalem
2015-2024
Stanford University
2018-2024
Organogenesis (United States)
2019-2024
Neuroscience Institute
2023
Neurosciences Institute
2020-2022
Abstract Self-organizing neural organoids represent a promising in vitro platform with which to model human development and disease 1–5 . However, lack the connectivity that exists vivo, limits maturation makes integration other circuits control behaviour impossible. Here we show stem cell-derived cortical transplanted into somatosensory cortex of newborn athymic rats develop mature cell types integrate sensory motivation-related circuits. MRI reveals post-transplantation organoid growth...
Abstract Timothy syndrome (TS) is a severe, multisystem disorder characterized by autism, epilepsy, long-QT and other neuropsychiatric conditions 1 . TS type (TS1) caused gain-of-function variant in the alternatively spliced developmentally enriched CACNA1C exon 8A, as opposed to its counterpart 8. We previously uncovered several phenotypes neurons derived from patients with TS1, including delayed channel inactivation, prolonged depolarization-induced calcium rise, impaired interneuron...
Abstract Background Within 2 min of severe ischemia, spreading depolarization (SD) propagates like a wave through compromised gray matter the higher brain. More SDs arise over hours in adjacent tissue, expanding neuronal damage. This period represents therapeutic window to inhibit SD and so reduce impending tissue injury. Yet most neuroscientists assume that course early brain injury can be explained by glutamate excitotoxicity, concept immediate release promotes downstream There are many...
Abstract Neural circuits connecting the cerebral cortex, basal ganglia and thalamus are fundamental networks for sensorimotor processing their dysfunction has been consistently implicated in neuropsychiatric disorders 1-9 . These recursive, loop have investigated animal models by clinical neuroimaging, however, direct functional access to developing human neurons forming these limited. Here, we use pluripotent stem cells reconstruct an vitro cortico-striatal-thalamic-cortical circuit...
Abstract Abnormalities in crosstalk between the thalamus and cerebral cortex are thought to lead severe neuropsychiatric disorders, such as epilepsy psychotic disorders. Pathogenic variants CACNA1G gene, which encodes α1G subunit of thalamus-enriched T-type voltage-gated calcium channel CaV3.1, associated with absence seizures, intellectual disability, schizophrenia, but cellular circuit level consequences these genetic humans remain unknown. Here, we developed an vitro human assembloid...
Life histories of oviparous species dictate high metabolic investment in the process gonadal development leading to ovulation. In vertebrates, these two distinct processes are controlled by gonadotropins follicle-stimulating hormone (FSH) and luteinizing (LH), respectively. While it was suggested that a common secretagogue, gonadotropin-releasing (GnRH), oversees both functions, generation loss-of-function fish challenged this view. Here, we reveal satiety cholecystokinin (CCK) is primary...
Modern, high-density neuronal recordings reveal at ever higher precision how information is represented by neural populations. Still, we lack the tools to understand these processes bottom-up, emerging from biophysical properties of neurons, synapses, and network structure. The concept dynamic gain function, a spectrally resolved approximation population's coding capability, has potential link cell-level network-level performance. However, not only useful but also very complex because gain's...
Life histories of oviparous species dictate high metabolic investment in the process gonadal development leading to ovulation. In vertebrates, these two distinct processes are controlled by gonadotropins follicle-stimulating hormone (FSH) and luteinizing (LH), respectively. While it was suggested that a common secretagogue, gonadotropin-releasing (GnRH), oversees both functions, generation loss-of-function fish challenged this view. Here we reveal satiety cholecystokinin (CCK) is primary...
Cortical regions that are damaged by insults, such as ischemia, hypoxia, and trauma, frequently generate spreading depolarization (SD). At the neuronal level, SDs entail complete breakdown of ionic gradients, persisting for seconds to minutes. It is unclear whether these transient events have a more lasting influence on function. Here, we describe electrophysiological changes in cortical neurons after recovery from hypoxia-induced SD. When examined with standard measures excitability several...
Life histories of oviparous species dictate high metabolic investment in the process gonadal development leading to ovulation. In vertebrates, these two distinct processes are controlled by gonadotropins follicle-stimulating hormone (FSH) and luteinizing (LH), respectively. While it was suggested that a common secretagogue, gonadotropin-releasing (GnRH), oversees both functions, generation loss-of-function fish challenged this view. Here, we reveal satiety cholecystokinin (CCK) is primary...
Abstract Life histories of oviparous species dictate high metabolic investment in the process gonadal development leading to ovulation. In vertebrates, these two distinct processes are controlled by gonadotropins follicle-stimulating hormone (FSH) and luteinizing (LH), respectively. While it was suggested that a common secretagogue, gonadotropin-releasing (GnRH), oversees both functions, generation loss-of-function fish challenged this view. Here, we reveal satiety cholecystokinin (CCK) is...
In whole-cell patch clamp recordings from layer 5 neocortical neurons, blockade of voltage gated sodium and calcium channels leaves a cesium current that is outward rectifying. This was originally identified as "non-specific cationic current", subsequently it hypothesized mediated by TRP channels. order to test this hypothesis, we used fluorescence imaging intracellular indicators, found no evidence suggest associated with influx either these ions the cell body or dendrites. Moreover, still...
SUMMARY Defects in interneuron migration during forebrain development can disrupt the assembly of cortical circuits and have been associated with neuropsychiatric disease. The molecular cellular bases such deficits particularly difficult to study humans due limited access functional tissue from patients. We previously developed a human assembloid model Timothy Syndrome (TS), caused by gain-of-function mutation CACNA1C which encodes L-type calcium channel (LTCC) Ca v 1.2. By functionally...