A5083123719- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Alzheimer's disease research and treatments
- Ion channel regulation and function
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Cholinesterase and Neurodegenerative Diseases
- stochastic dynamics and bifurcation
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Organic Chemistry Cycloaddition Reactions
- Asymmetric Synthesis and Catalysis
- Advanced Fluorescence Microscopy Techniques
- Synthetic Organic Chemistry Methods
- Memory and Neural Mechanisms
- Epilepsy research and treatment
- Prion Diseases and Protein Misfolding
- Radiomics and Machine Learning in Medical Imaging
- Electrochemical Analysis and Applications
- Cell Image Analysis Techniques
- Cardiac electrophysiology and arrhythmias
- Reproductive Biology and Fertility
- Erythrocyte Function and Pathophysiology
- Carbohydrate Chemistry and Synthesis
University of South Florida
2016-2025
University of Bonn
2024
University of Tampa
2018-2020
The Ohio State University
2014
Los Alamos National Laboratory
2011-2013
Pennsylvania State University
2007-2010
Ohio University
2005-2009
Wellcome Trust
1989-2004
East Cheshire NHS Trust
2001
Arkansas Agricultural Experiment Station
1996
Many radiomics features were originally developed for non-medical imaging applications and therefore original assumptions may need to be reexamined. In this study, we investigated the impact of slice thickness pixel spacing (or size) on extracted from Computed Tomography (CT) phantom images acquired with different scanners as well acquisition reconstruction parameters. The dependence CT texture gray-level discretization was also evaluated.A composed 10 cartridges materials scanned eight...
The pathological phenomena of seizures and spreading depression have long been considered separate physiological events in the brain. By incorporating conservation particles charge, accounting for energy required to restore ionic gradients, we extend classic Hodgkin–Huxley formalism uncover a unification neuronal membrane dynamics. examining dynamics as function potassium oxygen, now account wide range activities, from spikes seizures, (whether high or hypoxia induced), mixed seizure states,...
Abstract Radiomic features are potential imaging biomarkers for therapy response assessment in oncology. However, the robustness of with respect to parameters is not well established. Previously identified were found be intrinsically dependent on voxel size and number gray levels (GLs) a recent texture phantom investigation. Here, we validate GL in-phantom normalizations lung tumors. Eighteen patients non-small cell cancer varying tumor volumes analyzed. To compare patient data, scans...
Simultaneous analysis of oligomer and fibril assembly kinetics reveals inhibitory effects metastable oligomers on amyloid formation.
Observability of a dynamical system requires an understanding its state-the collective values variables. However, existing techniques are too limited to measure all but small fraction the physical variables and parameters neuronal networks. We constructed models biophysical properties membrane, synaptic, microenvironment dynamics, incorporated them into model-based predictor-controller framework from modern control theory. demonstrate that it is now possible meaningfully estimate dynamics...
Nonlinear ensemble state estimation offers a paradigm-shifting improvement in our ability to observe, predict, and control the of spiking neuronal systems. We use an Kalman filter predict hidden states future trajectories Hodgkin-Huxley equations, reconstruct ion dynamics, activity including strategy for dynamic conductance clamping, show feasibility controlling pathological cellular such as seizures.
Electrophysiological recordings show intense neuronal firing during epileptic seizures leading to enhanced energy consumption. However, the relationship between oxygen metabolism and seizure patterns has not been well studied. Recent studies have developed fast quantitative techniques measure microdomain concentration events. In this article, we develop a biophysical model that accounts for these experimental observations. The is an extension of Hodgkin-Huxley formalism includes...
Cell volume changes are ubiquitous in normal and pathological activity of the brain. Nevertheless, we know little how cell affects neuronal dynamics. We here performed first detailed study effects on By incorporating swelling together with dynamic ion concentrations oxygen supply into Hodgkin-Huxley type spiking dynamics, demonstrate spontaneous transition between epileptic seizure spreading depression states as swells contracts response to osmotic pressure. Our use an order parameter...
Abstract Background Within 2 min of severe ischemia, spreading depolarization (SD) propagates like a wave through compromised gray matter the higher brain. More SDs arise over hours in adjacent tissue, expanding neuronal damage. This period represents therapeutic window to inhibit SD and so reduce impending tissue injury. Yet most neuroscientists assume that course early brain injury can be explained by glutamate excitotoxicity, concept immediate release promotes downstream There are many...
Abstract Vesicular glutamate transporters accumulate in synaptic vesicles, where they also function as a major Cl - efflux pathway. Here we combine heterologous expression and cellular electrophysiology with mathematical modeling to understand the mechanisms underlying this dual of rat VGLUT1. When is main cytoplasmic anion, VGLUT1 functions H + -glutamate exchanger, transport rate around 600 s −1 at −160 mV. Transport other large anions, including aspartate, not stoichiometrically coupled...
Alzheimer's disease (AD) is characterized by profound disruption of synaptic function, with mounting evidence suggesting that amyloid-β (Aβ) oligomers disrupt calcium (Ca 2+ ) homeostasis through membrane pore formation. While these pores are known to alter intracellular Ca dynamics, their immediate impact on transmission and potential interaction Familial AD (FAD)-associated endoplasmic reticulum (ER) dysfunction remains unclear. Here, we extend our previously developed model presynaptic...
Cell volume changes are ubiquitous in normal and pathological activity of the brain. Nevertheless, we know little about dynamics cell tissue swelling, differential volumes neurons glia during states such as spreading depolarizations (SD) under ischemic non–ischemic conditions, epileptic seizures. By combining Hodgkin–Huxley type spiking dynamics, dynamic ion concentrations, simultaneous neuronal astroglial into a comprehensive model, elucidate why glial cells swell more than SD special case...
Alzheimer's disease (AD) is characterized by the abnormal proteolytic processing of amyloid precursor protein, resulting in increased production a self-aggregating form beta (Aβ). Several lines work on AD patients and transgenic mice with high Aβ levels exhibit altered rhythmicity, aberrant neuronal network activity hyperexcitability reflected clusters hyperactive neurons, spontaneous epileptic activity. Recent studies highlight that accumulation changes intrinsic properties inhibitory which...
Large variability in computed tomography (CT) radiomics feature values due to CT imaging parameters can have subsequent implications on the prognostic or predictive significance of these features. Here, we investigated impact pitch, dose, and reconstruction kernel radiomic Moreover, introduced correction factors reduce by kernels. The credence cartridge American College Radiology (ACR) phantoms were scanned five different scanners. ACR phantom was used for 3-D noise power spectrum (NPS)...
The inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) channel is crucial for the generation and modulation of intracellular Ca2+ signals in animal cells. To gain insight into complicated ligand regulation this ubiquitous channel, we constructed a simple quantitative continuous-time Markov-chain model from data. Our accounts most experimentally observed gating behaviors single native IP3R channels insect Sf9 Ligand (Ca2+ IP3) dependencies activity established six main ligand-bound complexes,...
Simultaneous changes in ion concentrations, glutamate, and cell volume together with exchange of matter between network vasculature are ubiquitous numerous brain pathologies. A complete understanding pathological conditions as well normal function, therefore, hinges on elucidating the molecular cellular pathways involved these mostly interdependent variations. In this paper, we develop first computational framework that combines Hodgkin–Huxley type spiking dynamics, dynamic concentrations...