A5035557125- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
Forschungszentrum Jülich
2020-2023
Abstract Vesicular glutamate transporters accumulate in synaptic vesicles, where they also function as a major Cl - efflux pathway. Here we combine heterologous expression and cellular electrophysiology with mathematical modeling to understand the mechanisms underlying this dual of rat VGLUT1. When is main cytoplasmic anion, VGLUT1 functions H + -glutamate exchanger, transport rate around 600 s −1 at −160 mV. Transport other large anions, including aspartate, not stoichiometrically coupled...
Excitatory amino acid transporters (EAATs) are prototypical dual function proteins that as coupled glutamate/Na+/H+/K+ and anion-selective channels. Both transport functions intimately intertwined at the structural level: Secondary active glutamate is based on elevator-like movements of mobile domain across membrane, lateral movement this results in anion channel opening. This particular gating mechanism predicts existence mutant with changed properties, but without alteration transport. We...