A5066686261- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Ion Transport and Channel Regulation
- Cardiac electrophysiology and arrhythmias
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Particle physics theoretical and experimental studies
- Molecular Sensors and Ion Detection
- Lipid Membrane Structure and Behavior
- Nicotinic Acetylcholine Receptors Study
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Genetics and Neurodevelopmental Disorders
- Advanced biosensing and bioanalysis techniques
- Particle Accelerators and Free-Electron Lasers
- Quantum Chromodynamics and Particle Interactions
- Invertebrate Immune Response Mechanisms
- High-Energy Particle Collisions Research
- Nuclear physics research studies
- Insect and Pesticide Research
- Genomics and Rare Diseases
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Chemical Synthesis and Analysis
- Drug Transport and Resistance Mechanisms
Forschungszentrum Jülich
2016-2025
Stadtwerke Jülich (Germany)
2023
Ludwig-Maximilians-Universität München
2023
Direct delivery of proteins and peptides into living mammalian cells has been accomplished using phospholipid liposomes as carrier particles. Such are usually taken up via endocytosis where the main part their cargo is degraded in lysosomes before reaching its destination. Here, fusogenic liposomes, a newly developed molecular system, were used for protein delivery. When such loaded with water-soluble brought contact cells, liposomal membrane efficiently fused cellular plasma delivering...
Somatic mutations are common in cancer, with only a few driving the progression of disease, while most silent passengers. Some may hinder or even reverse cancer progression. The voltage-gated proton channel (HV1) plays key role cellular pH homeostasis and shows increased expression several malignancies. Inhibiting HV1 cells reduces invasion, migration, extrusion, recovery, impacting tumor Focusing on HVCN1, gene coding for human (hHV1), 197 were identified from three databases: 134 missense...
Article10 September 2019Open Access Transparent process Allosteric gate modulation confers K+ coupling in glutamate transporters Daniel Kortzak orcid.org/0000-0001-9098-3466 Institute of Complex Systems, Zelluläre Biophysik (ICS-4) and JARA-HPC, Forschungszentrum Jülich, Germany Search for more papers by this author Claudia Alleva Ingo Weyand David Ewers Klinik für klinische Neurophysiologie, Universitätsmedizin Göttingen, Abteilung Neurogenetik, Max-Planck-Institut Experimentelle Medizin,...
Early/late endosomes, recycling and lysosomes together form the endo-lysosomal pathway. This system plays a crucial role in cell differentiation survival, dysregulation of appears to be important pathogenesis neurodevelopmental neurodegenerative diseases. Each compartment fulfils specific function, which is supported by ion transporters channels that modify concentrations electrical gradients across membranes. CLC-type Cl – /H + exchangers are group assumed regulate luminal acidification...
ClC-4 is an intracellular Cl−/H+ exchanger that highly expressed in the brain and whose dysfunction has been linked to intellectual disability epilepsy. Here we studied subcellular localization of human heterologous expression systems. retained endoplasmic reticulum (ER) upon overexpression HEK293T cells. Co-expression with distinct ClC-3 splice variants targets late endosome/lysosomes (ClC-3a ClC-3b) or recycling endosome (ClC-3c). When cultured astrocytes, sorted endocytic compartments WT...
The voltage-gated proton channel 1 (HV 1) is an important component of the cellular extrusion machinery and essential for charge compensation during respiratory burst phagocytes. HV has been identified in a wide range eukaryotes throughout animal kingdom, with exception insects. Therefore, it proposed that insects do not possess channel. In present study, we report existence 1-type We searched insect transcriptome shotgun assembly (TSA) sequence databases found putative orthologues various...
(Δ)pH induces conformational change from a deactivated to an activated state of the hHv1 voltage-gated proton channel.
The HV 1 voltage-gated proton (HV 1) channel is a key component of the cellular extrusion machinery and pivotal for charge compensation during respiratory burst phagocytes. best-described physiological inhibitor Zn2+ . Externally applied ZnCl2 drastically reduces currents reportedly recorded in Homo sapiens, Rattus norvegicus, Mus musculus, Oryctolagus cuniculus, Rana esculenta, Helix aspersa, Ciona intestinalis, Coccolithus pelagicus, Emiliania huxleyi, Danio rerio, Helisoma trivolvis,...
Abstract Objective Mutations in the gene solute carrier family member 1A2 ( SLC1A2) encoding excitatory amino acid transporter 2 (EAAT2) are associated with severe forms of epileptic encephalopathy. EAAT2 is expressed glial cells and presynaptic nerve terminals represents main l ‐glutamate uptake mammalian brain. It does not only function as a secondary active glutamate transporter, but also an anion channel. How naturally occurring mutations affect these two transport functions how such...
Voltage-gated ion channels, whose first identified function was to generate action potentials, are divided into subfamilies with numerous members. The family of voltage-gated proton channels (H
Excitatory amino acid transporters (EAATs) are prototypical dual function proteins that as coupled glutamate/Na+/H+/K+ and anion-selective channels. Both transport functions intimately intertwined at the structural level: Secondary active glutamate is based on elevator-like movements of mobile domain across membrane, lateral movement this results in anion channel opening. This particular gating mechanism predicts existence mutant with changed properties, but without alteration transport. We...
Voltage-gated proton channels (HV 1) are expressed in eukaryotes, including basal hexapods and polyneopteran insects. However, currently, there is little known about HV 1 A characteristic aspartate (Asp) that functions as the selectivity filter (SF) RxWRxxR voltage-sensor motif conserved structural elements channels. By analysing Transcriptome Shotgun Assembly (TSA) databases, we found 33 species meeting these requirements. Unexpectedly, an unusual natural variation Asp to glutamate (Glu) at...
${K}^{+}$-meson production in $\mathrm{pA}$ ( $A\phantom{\rule{0ex}{0ex}}=\phantom{\rule{0ex}{0ex}}\mathrm{C},\mathrm{Cu},\mathrm{Au}$) collisions has been studied using the ANKE spectrometer at an internal target position of COSY-J\"ulich accelerator. The complete momentum spectrum kaons emitted forward angles, $\ensuremath{\vartheta}\ensuremath{\le}12\ifmmode^\circ\else\textdegree\fi{}$, measured for a beam energy ${T}_{p}\phantom{\rule{0ex}{0ex}}=\phantom{\rule{0ex}{0ex}}1.0\mathrm{GeV}$,...
ClC-3, ClC-4, and ClC-5 are electrogenic chloride/proton exchangers that can be found in endosomal compartments of mammalian cells. Although the association with genetic diseases severe phenotype knock-out animals illustrate their physiological importance, cellular functions these proteins have remained insufficiently understood. We here study role two Clcn3 splice variants, ClC-3b ClC-3c, granular exocytosis catecholamine accumulation adrenal chromaffin cells using a combination...
Recently, three proton channels (HV ) have been identified and characterized in Aplysia californica (AcHV 1-3). Focusing on AcHV 1 2, analysis of Transcriptome Shotgun Assembly genomic databases 91 molluscs HV homologous other molluscs: to 2 were found 90 species (56 full-length sequences) 33 (18 sequences), respectively. Here, we report the discovery a fourth distinct channel family, 4. This new family has high homology was only bivalvian (13 species, 12 sequences). Typically, these possess...
S-Nitrosylation of cysteine residues is an important molecular mechanism for dynamic, post-translational regulation several proteins, providing a ubiquitous redox regulation. Cys are present in fluorescent proteins (FP), including members the family Aequorea victoria Green Fluorescent Protein (GFP)-derived FPs, where two highly conserved contribute to favorable environment autocatalytic chromophore formation reaction. The effect nitric oxide on fluorescence properties FPs has not been...