A5062287462- Ion channel regulation and function
- Advanced Electron Microscopy Techniques and Applications
- Ion Transport and Channel Regulation
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Advanced Biosensing Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Hearing, Cochlea, Tinnitus, Genetics
- Genetics and Neurodevelopmental Disorders
- Olfactory and Sensory Function Studies
- Force Microscopy Techniques and Applications
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Pain Mechanisms and Treatments
- Cellular transport and secretion
- RNA regulation and disease
- Neuroscience and Neuropharmacology Research
- Neurological disorders and treatments
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Trace Elements in Health
- Plant-based Medicinal Research
- ATP Synthase and ATPases Research
- Genomics and Rare Diseases
- Insect and Pesticide Research
Forschungszentrum Jülich
2014-2024
Stadtwerke Jülich (Germany)
2023
Ludwig-Maximilians-Universität München
2023
ORCID
2022
Marine Biological Laboratory
2014
Guanylyl cyclases (GCs), which synthesize the messenger cyclic guanosine 3′,5′-monophosphate, control several sensory functions, such as phototransduction, chemosensation, and thermosensation, in many species from worms to mammals. The GC chemoreceptor sea urchin sperm can decode chemoattractant concentrations with single-molecule sensitivity. molecular cellular underpinnings of ultrasensitivity are not known for any eukaryotic chemoreceptor. In this paper, we show that an exquisitely high...
Early/late endosomes, recycling and lysosomes together form the endo-lysosomal pathway. This system plays a crucial role in cell differentiation survival, dysregulation of appears to be important pathogenesis neurodevelopmental neurodegenerative diseases. Each compartment fulfils specific function, which is supported by ion transporters channels that modify concentrations electrical gradients across membranes. CLC-type Cl – /H + exchangers are group assumed regulate luminal acidification...
ClC-4 is an intracellular Cl−/H+ exchanger that highly expressed in the brain and whose dysfunction has been linked to intellectual disability epilepsy. Here we studied subcellular localization of human heterologous expression systems. retained endoplasmic reticulum (ER) upon overexpression HEK293T cells. Co-expression with distinct ClC-3 splice variants targets late endosome/lysosomes (ClC-3a ClC-3b) or recycling endosome (ClC-3c). When cultured astrocytes, sorted endocytic compartments WT...
In the mammalian ear, transduction of sound stimuli is initiated by K+ entry through mechano-sensitive channels into inner hair cells. driven a positive endocochlear potential that maintained marginal cell layer stria vascularis. This process requires basolateral import NKCC1 Na+-2Cl--K+ co-transporters as well Cl- efflux ClC-Ka/barttin or ClC-Kb/barttin channels. Multiple mutations in gene encoding obligatory CLC-K subunit barttin, BSND, have been identified patients with Bartter syndrome...
The HV 1 voltage-gated proton (HV 1) channel is a key component of the cellular extrusion machinery and pivotal for charge compensation during respiratory burst phagocytes. best-described physiological inhibitor Zn2+ . Externally applied ZnCl2 drastically reduces currents reportedly recorded in Homo sapiens, Rattus norvegicus, Mus musculus, Oryctolagus cuniculus, Rana esculenta, Helix aspersa, Ciona intestinalis, Coccolithus pelagicus, Emiliania huxleyi, Danio rerio, Helisoma trivolvis,...
The oligomeric state of plasma membrane proteins is the result interactions between individual subunits and an important determinant their function. Most approaches used to address this question rely on extracting these complexes from native environment, which may disrupt weaker interactions. Therefore, microscopy techniques have been increasingly in recent years determine states situ. Classical light suffers insufficient resolution, but super-resolution methods such as single molecule...
ClC-3 Cl – /H + exchangers are expressed in multiple endosomal compartments and likely modify intra-endosomal pH [Cl ] via the stoichiometrically coupled exchange of two ions one H . We studied pain perception Clcn3 –/– mice found that not only modifies electrical activity peripheral nociceptors but is also involved inflammatory processes spinal cord. demonstrate regulates number Na v K ion channels plasma membrane dorsal root ganglion (DRG) neurons these changes impair age-dependent decline...
Barttin is an accessory subunit of ClC-K chloride channels expressed in the kidney and inner ear. Main functions ClC-K/barttin are generation cortico-medullary osmotic gradients endocochlear potential Mutations gene encoding barttin, BSND, result impaired urinary concentration sensory deafness. predicted to be a two helical integral membrane protein that directly interacts with its ion channel bilayer where it stabilizes complex, promotes incorporation into surface leads activation. It...
Abstract The solute carrier family 26 (SLC26) encompasses multifunctional anion exchangers in all kingdoms of life. SLC26 proteins are known to assemble as dimers, and co-expression multiple isoforms certain cells raises the question whether different SLC26s can into hetero-dimers. We focused on SLC26A11, a broadly expressed isoform that differs from other its subcellular localization. Whereas vast majority SLC26-FP fusion proteins, i.e. SLC26A1, SLC26A2, SlC26A3, SLC26A4/pendrin,...
Determining the oligomeric state of membrane proteins is critical for understanding their function. However, traditional ex situ methods like clear native gel electrophoresis can disrupt protein subunit interactions during sample preparation. In such as stepwise photobleaching have limitations due to high expression levels and optical resolution in microscopy. Super-resolution microscopy techniques single-molecule localization (SMLM) potential overcome these limitations, but stochastic...
Background: Variants in CLCN4 are implicated neurodevelopmental disorder, X-linked intellectual disability, and epileptic encephalopathy. encodes ClC-4, which is hypothesized to play a role ion homeostasis intracellular trafficking. ClC-4 relies on its formation of heterodimers with ClC-3, possesses signals for target organelles. Methods: Case-Series. Then, we performed heterologous expression, patch-clamp electrophysiology, confocal microscopy, protein biochemistry experiments characterize...
<title>Abstract</title> <italic>CLCN4</italic>-related disorder is a rare X-linked neurodevelopmental condition with pathogenic mechanism yet to be elucidated. <italic>CLCN4 </italic>encodes the vesicular 2Cl<sup>-</sup>/H<sup>+</sup> exchanger ClC-4, and <italic>CLCN4</italic> variants frequently result in altered ClC-4 transport activity<italic>. </italic>The precise cellular molecular function of remains unknown; however, together ClC-3, thought have role ion homeostasis endosomes...
The oligomeric state of plasma membrane proteins is the result interactions between individual and an important determinant their function. Most approaches used to address this question rely on extracting these complexes from native environment, which may disrupt weaker interactions. Therefore, microscopy techniques have been increasingly in recent years determine states situ . Classical light suffers insufficient resolution, but super-resolution methods such as single molecule localization...