The KISS1 gene encodes the kisspeptin neuropeptide, which activates receptor (KISS1R; G protein-coupled 54; GPR54) and participates in neuroendocrine regulation of GnRH secretion. To study physiological function(s) evolutionary conservation KISS1, we cloned opossum, Xenopus, zebrafish kiss1 cDNAs. Processing zebrafish, or opossum KISS proteins would liberate a carboxy-terminal amidated peptide with 52, 54, 53 amino acid residues, respectively. Phylogenetic analysis all known vertebrate...

Kisspeptin is an important regulator of reproduction in many vertebrates. The involvement the two kisspeptins, Kiss1 and Kiss2, their receptors, Gpr54-1 Gpr54-2, controlling was studied brains modern teleosts, striped hybrid basses. In situ hybridization laser capture microdissection followed by quantitative RT (QRT)-PCR detected coexpression kiss1 kiss2 hypothalamic nucleus lateral recess. Neurons expressing gpr54-1 gpr54-2 were several brain regions. preoptic area, colocalized GnRH1...

The endocrine regulation of vertebrate reproduction is achieved by the coordinated actions several peptide neurohormones, tachykinin among them. To study evolutionary conservation and physiological functions neurokinin B (NKB), we identified ( tac ) receptor (NKBR) genes from many fish species, cloned two cDNA forms zebrafish. Phylogenetic analysis showed that piscine Tac3s mammalian arise one lineage. High identity was found different species in region encoding NKB; all shared common...

ABSTRACT A lethal disease of koi and common carp (species Cyprinus carpio ) has afflicted many fish farms worldwide since 1998, causing severe financial losses. Morbidity mortality are restricted to appear in spring autumn, when water temperatures 18 28°C. We have isolated the virus from sick fish, propagated it fin cell culture, shown that a single clone causes upon infection. Intraperitoneal injection or bathing virus-containing kills 85 100% within 7 21 days. This is similar previously...

The metallic luster from the skin of fish is due to a photonic crystal system composed multilayer stacks cytoplasm and crystals. crystals are described as thin (50−100 nm) plates guanine, with no reference their hydration state. We established through X-ray diffraction that structure anhydrous guanine. noted structure-function relationship exceptional compared other purines similar molecular stacking structure. These elongate in direction stacking, contrast biogenic guanine whose smallest...

In fish, FSH is generally important for early gonadal development and vitellogenesis. As in mammals, a heterodimer composed of an alpha subunit that noncovalently associated with the hormone-specific beta subunit. The objective present study was to express glycosylated, properly folded, biologically active tilapia (tFSH) using Pichia pastoris expression system. Using this material, we aimed develop specific ELISA enable response GnRH. methylotrophic yeast P. used coexpress recombinant genes...

LPXRFamide (LPXRFa) peptides have been characterized for their ability to inhibit gonadotropin (GTH) release in birds and stimulate growth hormone (GH) frogs. However, involvement regulating the reproductive hypothalamo-pituitary-gonadal axis mammals fish is inconclusive. To study role of LPXRFa regulation GTH secretion, we cloned tilapia LPXRF receptor (LPXRF-R). Processing preproLPXRFa liberated three mature that varied size post-translational modifications. Phylogenetic analysis closely...

The function and components of the hypothalamic-pituitary axis are conserved among vertebrates; however, in fish, a neuroglandular mode delivery (direct contact between axons endocrine cells) was considered dominant, whereas tetrapods hypothalamic signals relayed to their targets via hypophysial portal blood system (neurovascular mode). By using transgenic zebrafish model we studied functional anatomical aspects gonadotrope regulation thus revisiting existing model. FSH cells were found be...

Abstract Serotonergic psychedelics are emerging therapeutics for psychiatric disorders, yet their underlying mechanisms of action in the brain remain largely elusive. Here, we developed a wide-field behavioral tracking system larval zebrafish and investigated effects psilocybin, psychedelic serotonin receptor agonist. Machine learning analyses precise body kinematics identified latent states reflecting spontaneous exploration, visually-driven rapid swimming, irregular swim patterns following...

In cultured pituitary cells of tilapia, gonadotropin-releasing hormone (GnRH; IO11M 4-24 h), elevation cyclic AMP (by 10 µM forskolin or 0.2 mM3-isobutyl-1-methylxanthine: IBMX 0.5-36 h) activation protein kinase C (PKC; by 12.5 nM tetradecanoyl phorbol-13-acetate: TPA, 0.5–24 all increased gonadotropin (GtH) IIβ steady state mRNA levels three- to fourfold. The involvement PKA and PKC in the GnRH stimulatory effect on both GtH release was corroborated use inhibitors, H89 GF109203X,...

Althoughmolecular mechanisms underlying steroid effects on GnRH and dopamine receptors are well documented in mammals, little is known fish. Herein, we describe the expression of pituitary relative to gonadotropin release. We exposed female tilapia graded doses estradiol or 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP) vitro, vivo, determined mRNA levels ofgnrhr1,gnrhr3,drd2,lhb, andfshbby real-time PCR. also using specific ELISAs. Exposure low caused increasedgnrhr3mRNA vivo probably...

Abstract Background In oviparous vertebrates, including fish, vitellogenesis consists of highly regulated pathways involving 17β-estradiol (E2). Previous studies focused on a relatively small number hepatic expressed genes during vitellogenesis. This study aims to identify involved in and by E2, using zebrafish microarray gene expression profiling, provide information functional distinctive the liver vitellogenic female, as model fish. Results Genes associated with were revealed following...

Social position in a dominance hierarchy is often tightly coupled with fertility. Consequently, an animal that can recognize and rapidly take advantage of opportunity to rise rank will have reproductive advantage. Reproduction all vertebrates controlled by the brain-pituitary-gonad axis, males African cichlid fish Astatotilapia burtoni, GnRH1 neurons at apex this axis are under social control. However, little known about how quickly information transformed into functional change, or socially...

Retinal is the main retinoid stored in oviparous eggs of fish, amphibians, and reptiles, reaching oocytes association with vitellogenins, yolk precursor proteins. During early presegmentation stages zebrafish embryos, retinal metabolized to retinoic acid (RA), which regulates genes involved cell proliferation, differentiation, tissue function therefore essential for normal embryonic development. While synthesis vitellogenin its regulation by 17β-estradiol (E(2)) were extensively...

The gonadotropins follicle-stimulating hormone (FSH) and luteinizing (LH) are key regulators of the reproductive axis in vertebrates. Despite high popularity zebrafish as a model organism for studying functions, to date no transgenic with labeled gonadotropes have been introduced. Using gonadotropin regulatory elements from tilapia, we generated two lines gonadotropes. tilapia sequences were highly divergent but several conserved allowed promoters correctly drive transgenes pituitaries. FSH...