A5011793090- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Immune cells in cancer
- CAR-T cell therapy research
- Gut microbiota and health
- Clinical Nutrition and Gastroenterology
- Wound Healing and Treatments
Inserm
2018-2024
Institut Curie
2018-2024
Université Paris Sciences et Lettres
2018-2024
Immunité et Cancer
2023-2024
National Institute of Dental and Craniofacial Research
2023
National Institutes of Health
2023
Mucosal-associated invariant T (MAIT) cells are abundant with unique specificity for microbial metabolites. MAIT conservation along evolution indicates important functions, but their low frequency in mice has hampered detailed characterization. Here, we performed the first transcriptomic analysis of murine cells. MAIT1 (RORγtneg) and MAIT17 (RORγt+) subsets were markedly distinct from mainstream cells, quasi-identical to NKT1 NKT17 subsets. The expression similar programs was further...
Tissue repair processes maintain proper organ function following mechanical or infection-related damage. In addition to antibacterial properties, mucosal associated invariant T (MAIT) cells express a tissue transcriptomic program and promote skin wound healing when expanded. Herein, we use human-like mouse model of full-thickness excision assess the underlying mechanisms MAIT cell function. Single-cell RNA sequencing analysis suggested that already at steady state. Following excision,...
Abstract γδ T cells perform heterogeneous functions in homeostasis and disease across tissues. However, it is unclear whether these roles correspond to distinct subsets or a homogeneous population of exerting context-dependent functions. Here, by cross-organ multimodal single-cell profiling, we reveal that various mouse tissues harbor unique site-adapted subsets. Epidermal intestinal intraepithelial are transcriptionally exhibit epigenetic hallmarks functional diversity. Through parabiosis...
Mucosal-associated invariant T (MAIT) cells harbor evolutionarily conserved TCRs, suggesting important functions. As human and mouse MAIT functional programs appear distinct, the features remain unidentified. Using species-specific tetramers coupled to single-cell RNA sequencing, we characterized cell development in six species spanning 110 million years of evolution. Cross-species analyses revealed transcriptional events underlying maturation, marked by ZBTB16 induction all species. human,...
Intestinal inflammation shifts microbiota composition and metabolism. How the host monitors responds to such changes remains unclear. Here, we describe a protective mechanism by which mucosal-associated invariant T (MAIT) cells detect metabolites produced upon intestinal promote tissue repair. At steady state, MAIT ligands derived from riboflavin biosynthesis pathway were aerotolerant bacteria residing in colonic mucosa. Experimental colitis triggered luminal expansion of...
Generating knockout mice for target molecules in specific T cell populations, without subset-specific promoters, is time-consuming and costly. Here, we describe steps enriching mucosal-associated invariant cells from the thymus, expanding them vitro performing a CRISPR-Cas9 knockout. We then detail procedure injecting into wounded Cd3ε−/− characterizing skin. For complete details on use execution of this protocol, please refer to du Halgouet et al. (2023).1
Tissue repair processes maintain proper organ function following mechanical or infection‑related damage. In addition to anti-bacterial properties, MAIT cells express a tissue transcriptomic program and promote skin wound healing when expanded. Herein, we use human‑like full‑thickness excision mouse model assess the underlying mechanisms of cell function. Single-cell RNAseq analysis suggests that already at steady state. Following excision, keratinocyte proliferation thereby accelerating...