A5053644134- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- HIV Research and Treatment
- Infection Control and Ventilation
- Long-Term Effects of COVID-19
- Viral gastroenteritis research and epidemiology
- COVID-19 and Mental Health
- Insect symbiosis and bacterial influences
- Virus-based gene therapy research
- Virology and Viral Diseases
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Vector-borne infectious diseases
- COVID-19 Impact on Reproduction
- Viral Infections and Immunology Research
- Bacillus and Francisella bacterial research
- Cytomegalovirus and herpesvirus research
- Research on Leishmaniasis Studies
Institut de Recherche pour le Développement
2012-2024
Aix-Marseille Université
2012-2024
Inserm
2016-2024
Méditerranée Infection Foundation
2014-2023
Santé Publique France
2021
Institut National de Santé Publique
2021
Assistance Publique Hôpitaux de Marseille
2015-2020
École des Hautes Études en Santé Publique
2014-2017
Institut de Neurobiologie de la Méditerranée
2003-2012
Institut de Recherche pour le Développement
2010
Coronavirus disease 2019 (COVID-19) is a caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has resulted in pandemic1. The C5a complement factor its receptor C5aR1 (also known as CD88) have key role the initiation maintenance of several inflammatory responses recruiting activating neutrophils monocytes1. Here we provide longitudinal analysis immune responses, including phenotypic analyses cells assessments soluble factors that are present blood...
Infection with yellow fever virus (YFV), the prototypic mosquito-borne flavivirus, causes severe febrile disease haemorrhage, multi-organ failure and a high mortality. Moreover, in recent years Flavivirus genus has gained further attention due to re-emergence increasing incidence of West Nile, dengue Japanese encephalitis viruses. Potent safe antivirals are urgently needed. Starting from crystal structure NS3 helicase Kunjin (an Australian variant Nile virus), we identified novel,...
After an infection by SARS-CoV-2, many patients present with persistent physical symptoms that may impair their quality of life. Beliefs regarding the causes these influence perception and promote maladaptive health behaviors.
T-705, also known as favipiravir, is a small-molecule inhibitor that currently in clinical development for the treatment of influenza virus infections. This molecule inhibits replication broad spectrum other RNA viruses. The objective this study was to investigate antiviral effect favipiravir on chikungunya (CHIKV) and contribute unravelling molecular mechanism action against virus. anti-CHIKV examined cell culture mouse model lethal infection. A five-step protocol used select CHIKV variants...
Abstract We spotted severe acute respiratory syndrome coronavirus 2 on polystyrene plastic, aluminum, and glass for 96 hours with without bovine serum albumin (3 g/L). observed a steady infectivity (<1 log10 drop) 3.5 decrease glass, 6 drop aluminum. The presence of proteins noticeably prolonged infectivity.
Background In 2014–2015, we assessed favipiravir tolerance and efficacy in patients with Ebola virus (EBOV) disease (EVD) Guinea (JIKI trial). Because the drug had never been used before for this indication that high concentrations of drugs were needed to achieve antiviral against EBOV, a pharmacokinetic model propose relevant dosing regimen. Here report plasma achieved participants JIKI trial put them perspective model-based targeted concentrations. Methods findings Pre-dose collected at...
Standard precautions to minimize the risk of SARS-CoV-2 transmission implies that infected cell cultures and clinical specimens may undergo some sort inactivation reduce or abolish infectivity. We evaluated three heat protocols (56 °C-30 min, 60 °C-60 min 92 °C-15 min) on using (i) culture supernatant, (ii) virus-spiked human sera (iii) nasopharyngeal samples according recommendations European norm NF EN 14476-A2. Regardless protocol type samples, a 4 Log10 TCID50 reduction was observed....
Abstract The resurgence of Chikungunya virus is described during an urban epidemic in Kinshasa Democratic Republic the Congo, after 39 years without any isolation virus. was isolated sera from nine patients with clinical symptoms. A 1,200 bp long partial sequence E1/3'UTR genomic region determined for each isolate. All sequences clustered central African lineage. They constitute reference Congo. J. Med. Virol. 74:277–282, 2004. © 2004 Wiley‐Liss, Inc.
Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal (virus-spiked clinical COVID-19 patients) were used measure reduction infectivity after 10 minute contact with lysis...
Abstract Clinical samples collected in COVID-19 patients are commonly manipulated BSL-2 laboratories for diagnostic purpose. We used the French norm NF-EN-14476+A2 derived from European standard EN-14885. To avoid risk of exposure laboratory workers, we showed that Triton-X100 must be added to guanidinium thiocyanate-lysis buffers obtain a 6-log reduction infectious virus. Although heating protocol consisting 92°C-15min was more effective rather than 56°C-30min and 60°C-60min achieve...
The replacement of the Omicron BA.1 variant SARS-CoV-2 by BA.2 and rapid growth BA.5 sub lineage, which have both different sets mutations in spike glycoprotein, alters spectrum activity therapeutic antibodies currently licensed European Union. Using clinical strains variants, we compared neutralising power monoclonal against BA.1, using an ancestral strain (lineage B.1, D614G) a Delta as reference. Sotrovimab/Vir-7831 is less active than (fold change reduction ~ 1,4) even 2.7). Within...
Importance Persistent symptoms after SARS-CoV-2 infection are an emerging public health problem. The duration of these remains poorly documented. Objective To describe the temporal dynamics persistent and factors associated with their resolution. Design, Setting, Participants This cross-sectional study involved 53 047 participants from 3 French adult population-based cohorts (CONSTANCES [Consultants des Centres d’Examens de Santé], E3N/E4N, Nutrinet-Santé) who were included in a nationwide...
Abstract We report the isolation of chikungunya virus from a patient during an outbreak denguelike syndrome in Cameroon 2006. The was phylogenetically grouped Democratic Republic Congo cluster, indicating continuous circulation genetically similar population 6 years Central Africa.
Background Chikungunya is an Aedes -borne disease characterised by febrile arthralgia and responsible for massive outbreaks. We present a prospective clinical cohort study retrospective serological relating to CHIK outbreak, in the Republic of Congo 2011. Methodology Findings analysed 317 suspected cases, which 308 (97.2%) lived city Brazzaville (66.6% South area). Amongst them, 37 (11.7%) were CHIKV+ve patients (i.e., biologically confirmed real-time RT-PCR assay), whom 36 (97.3%) had...
Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by commercial NS1 protein-based IgG ELISA, confirmation cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with dengue seroprevalence above 90%), this allowed reaching specificity sensitivity values over 98%. The CPE-based VNT consists of...
ABSTRACT A doxorubicin derivate, SA-17, that carries a squaric acid amide ester moiety at the carbohydrate (α- l -daunosaminyl) group was identified as selective inhibitor of in vitro dengue virus (DENV) serotype 2 replication (50% effective concentration [EC 50 ] = 0.34 ± 0.20 μg/ml [0.52 0.31 μM]). SA-17 is markedly less cytostatic than parent compound, resulting selectivity index value ∼100. also inhibits yellow fever 17D (YFV-17D) (EC 3.1 1.0 [4.8 1.5 μM]), although efficiently DENV...
Abstract This study reports the first isolation and partial genetic characterization of Chikungunya virus (CHIKV) from patients during a 2006–2007 dengue‐like syndrome outbreak in Gabon. The isolated viruses were phylogenetically close to strains Democratic Republic Congo 7 years ago more recently Cameroon. These results indicate continuing circulation genetically stable CHIKV population Central Africa. J. Med. Virol. 80:430–433, 2008. © 2008 Wiley‐Liss, Inc.
Arboviral infections have repeatedly been reported in the republic of Djibouti, consistent with fact that essential vectors for arboviral diseases are endemic region. However, there is a limited recent information regarding arbovirus circulation, and associated risk predictors to human exposure largely unknown. We performed, from November 2010 February 2011 Djibouti city general population, cross-sectional ELISA sero-neutralisation-based sero-epidemiological analysis nested household cohort,...