A5048640766- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Neuroblastoma Research and Treatments
- IL-33, ST2, and ILC Pathways
- Cancer Immunotherapy and Biomarkers
- Chemical Synthesis and Analysis
- MicroRNA in disease regulation
- Chemokine receptors and signaling
- Lung Cancer Research Studies
- Hematopoietic Stem Cell Transplantation
- Cell Adhesion Molecules Research
- 3D Printing in Biomedical Research
- Synthesis and Biological Evaluation
- Adenosine and Purinergic Signaling
- Cancer, Hypoxia, and Metabolism
- Click Chemistry and Applications
- Immune Response and Inflammation
- Receptor Mechanisms and Signaling
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
University of Genoa
2016-2025
Istituto Giannina Gaslini
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2017-2025
Instituto Nacional de Metrologia, Qualidade e Tecnologia
2021
Tecnologie Avanzate (Italy)
1995-2015
Tehran University of Medical Sciences
2012
National and Kapodistrian University of Athens
2012
Alleanza Contro il Cancro
1990-2006
Ente Ospedaliero Ospedali Galliera
2006
University of Florence
2005
Human natural killer (NK) cells express a series of activating receptors and coreceptors that are involved in recognition killing target cells. In this study, an attempt to identify the cellular ligands for such triggering surface molecules, mice were immunized with NK-susceptible On basis functional screening, four mAbs selected induced partial down-regulation NK-mediated cytotoxicity against immunizing As revealed by biochemical analysis, three recognized molecules ∼70 kD. The other mAb...
Two major receptors involved in human natural cytotoxicity, NKp46 and NKp44, have recently been identified. However, experimental evidence suggested the existence of additional such receptor(s). In this study, by generation monoclonal antibodies (mAbs), we identified NKp30, a novel 30-kD triggering receptor selectively expressed all resting activated killer (NK) cells. Although mAb-mediated cross-linking NKp30 induces strong NK cell activation, masking inhibits cytotoxicity against normal or...
After culture in interleukin (IL)-2, natural killer (NK) cells acquire an increased capability of mediating non–major histocompatibility complex (MHC)–restricted tumor cell lysis. This may reflect, at least part, the de novo expression by NK triggering receptors involved cytolysis. In this study we identified a novel 44-kD surface molecule (NKp44) that is absent freshly isolated peripheral blood lymphocytes but progressively expressed all vitro after IL-2. Different from other markers...
The surface density of the triggering receptors responsible for natural killer (NK)-mediated cytotoxicity is crucial ability NK cells to kill susceptible target cells. In this study, we show that transforming growth factor β1 (TGFβ1) down-regulates expression NKp30 and in part NKG2D but not other such as NKp46. TGFβ1-mediated inhibition reflects gene regulation at transcriptional level. has been shown represent major receptor involved NK-mediated killing dendritic Accordingly,...
Summary NKp46 has been shown to represent a novel, natural killer (NK) cell–specific surface molecule, involved in human NK cell activation. In this study, we further analyzed the role of cytotoxicity against different tumor target cells. We provide direct evidence that represents major activating receptor recognition and lysis both murine Although may cooperate with other receptors (including recently identified NKp44 molecule) induction NK-mediated cells, it only Molecular cloning cDNA...
Human CD3-16+56+ natural killer (NK) cells have been shown to display a clonally distributed ability recognize major histocompatibility complex (MHC) class I alleles. Opposite T lymphocytes, in NK cells, specific recognition of MHC molecules appears induce inhibition cytolytic activity and, thus, protect target cells. Since precise correlation has established between the expression NK-specific GL183 and EB6 surface (belonging novel p58 molecular family) specificity clones, we analyzed...
Limited information is available on the surface molecules that are involved in natural killer (NK) cell triggering. In this study, we selected BAB281 monoclonal antibody (mAb) basis of its ability to trigger NK-mediated target lysis. identified a novel NK cell–specific molecule 46 kD (p46) expressed by all resting or activated cells. Importantly, unlike antigens so far, expression p46 was strictly confined Upon mAb-mediated cross-linking, induced strong triggering leading [Ca2+]i increases,...
2B4 is a surface molecule involved in activation of the natural killer (NK) cell–mediated cytotoxicity. It binds protein termed Src homology 2 domain–containing (SH2D1A) or signaling lymphocyte (SLAM)-associated (SAP), which turn has been proposed to function as regulator 2B4-associated signal transduction pathway. In this study, we analyzed patients with X-linked lymphoproliferative disease (XLP), severe inherited immunodeficiency characterized by critical mutations SH2D1A gene and...
The natural killer (NK) cell-specific p58 molecules EB6 and GL183 have been shown to represent the putative surface receptors for two distinct groups of human histocompatibility leukocyte antigen (HLA) C alleles. Interaction between class I expressed on target cells results in inhibition NK-mediated cytolytic activity thus cell protection. In present study, we show that may also act as mediating NK triggering. Activatory were found be confined only certain donors. Moreover, these donors, a...
Surface receptors involved in natural killer (NK) cell triggering during the process of tumor lysis have recently been identified. Of these receptors, NKp44 is selectively expressed by IL-2– activated NK cells and may contribute to increased efficiency mediate lysis. Here we describe molecular cloning NKp44. Analysis cloned cDNA indicated that a novel transmembrane glycoprotein belonging Immunoglobulin superfamily characterized single extracellular V-type domain. The charged amino acid...
NKp46 is a novel triggering receptor expressed by all human NK cells that involved in natural cytotoxicity. In this study we show the surface density of may vary different and precise correlation exists between phenotype clones their cytotoxicity against HLA-class I-unprotected allogeneic or xenogeneic cells. Thus, NKp46bright efficiently lysed murine tumor while NKp46dull were poorly cytolytic both types target We also correlates with ability to lyse autologous Finally, was found be deeply...
In previous studies we identified a surface molecule (termed GL183) capable of mediating cell activation and selectively expressed by subset human CD3-CD16+ natural killer (NK) cells. this study analyzed whether other subset-specific functional molecules were in GL183- NK To end, mice immunized with the PE29 (CD3-CD16+GL183-) clone. Monoclonal antibodies (mAbs) selected screening hybridoma supernatants for their ability to trigger cytolytic activity clone against myelomonocytic leukemia...
Natural killer (NK) cells play a central role in host defense against various pathogens. Functional defects of NK HIV-1 infection as direct effect abnormal expression or function inhibitory receptors (iNKRs), activating natural cytotoxicity (NCRs), and NKG2D have not yet been described. This study demonstrates an expansion the functionally defective CD56 - /CD16 + population viremic versus aviremic patients. We also demonstrate that HIV-infected patients, iNKRs was well conserved most cases,...
Abstract In this study, cancer cells were isolated from tumor specimens of nine glioblastoma patients. Glioblastoma cells, cultured under suitable culture conditions, displayed markers typical neural stem capable partial multilineage differentiation in vitro, and gave origin to infiltrating tumors when orthotopically injected NOD/SCID mice. These although resistant freshly NK highly susceptible lysis mediated by both allogeneic autologous IL-2 (or IL-15)-activated cells. Indeed, all...
Human natural killer (NK) cells express inhibitory receptors that are specific for different groups of HLA-C or HLA-B alleles. The majority these belong to the immunoglobulin (Ig) superfamily and characterized by two three extracellular Ig-like domains. Here we describe a novel NK receptor is group HLA-A HLA-A3-specific cell clone DP7 has been used mice immunization. Two mAbs, termed Q66 Q241, bound immunizing stained only subset populations clones. Among mAb-reactive clones, further...
Natural killer cells express clonally distributed receptors specific for major histocompatibility complex class I molecules. The human leukocyte antigen (HLA)-C-specific have been molecularly identified and cloned. They exist not only as inhibitory (p58) but also activatory (p50) receptors. Here we show that p50 p58 are highly homologous in their extracellular regions formed by two Ig-like domains. In contrast, differences transmembrane cytoplasmic portions. Whereas p 58 displays a...
NKG2D is a recently described activating receptor expressed by both NK cells and CTL. In this study we investigated the role of in natural cytolysis mediated cell clones. The varied depending on type target analyzed. Lysis various tumors appeared to be exclusively cytotoxicity receptors (NCR) dependent. contrast, killing another group cells, including not only epithelial lines HELA IGROV-1, but also FO-1 melanoma, JA3 leukemia, Daudi Burkitt lymphoma even normal PHA-induced lymphoblasts,...