Elevation of the DNA-unwinding helicase RECQL4, which participates in various DNA repair pathways, has been suggested to contribute pathogenicity human cancers, including gastric cancer. In this study, we addressed prognostic and chemotherapeutic significance RECQL4 cancer, yet be determined. We observed significant increases mRNA or protein >70% three independent sets cancer specimens examined, relative normal tissues. Strikingly, high expression primary tumors correlated well with poor...

PRKN/parkin activation through phosphorylation of its ubiquitin and ubiquitin-like domain by PINK1 is critical in mitophagy induction for eliminating the damaged mitochondria. Deubiquitinating enzymes (DUBs) functionally reversing PRKN ubiquitination are controlling magnitude PRKN-mediated process. However, potential DUBs that directly target antagonize pro-mitophagy effect remains to be identified characterized. Here, we demonstrated USP33/VDU1 localized at outer membrane mitochondria...

Abstract Loss of TGFBI, a secreted protein induced by transforming growth factor-β, has been implicated in cell proliferation, tumor progression, and angiogenesis vitro studies. However, vivo antitumor functions TGFBI as well the underlying molecular mechanism are not understood. To these aims, we have generated mouse model with disruption genomic locus. Mice lacking show retarded prone to spontaneous tumors 7,12-dimethylbenz(a)anthracene–induced skin tumors. In relation wild-type (WT)...

Xeroderma pigmentosum group D (XPD/ERCC2) encodes an ATP-dependent helicase that plays essential roles in both transcription and nucleotide excision repair of nuclear DNA, however, whether or not XPD exerts similar functions mitochondria remains elusive. In this study, we provide the first evidence is localized inner membrane mitochondria, cells under oxidative stress showed enhanced recruitment into mitochondrial compartment. Furthermore, reactive oxygen species production levels...

The stability of foams in the presence oil is a crucial factor for foam displacement process enhanced recovery. After test mixtures sodium dodecyl sulfate (SDS) and dodecyltrimethylammonium bromide (C12TAB), "catanionic" surfactant are proposed as novel agent to enhance resistance foam. Based on investigations evolution interfacial properties, mechanisms demonstrated by means approaches multiple light scattering dilational rheological experiments. Because electrostatic attraction between...

Breast cancer occur both in hereditary and sporadic forms, the later one comprises an overwhelming majority of breast cases among women. Numerical structural alterations involving chromosome 8, with loss short arm (8p) gain long (8q), are frequently observed cells tissues. In this study, we show that most human tumor cell lines examined display over representation 8q24, a chromosomal locus RecQL4 is regionally mapped to, consequently, markedly elevated level expression. An increased mRNA was...

Human RecQL4 helicase plays critical roles in the maintenance of genomic stability. Mutations results three clinically related autosomal recessive disorders: Rothmund-Thomson syndrome (RTS), RAPADILINO, and Baller-Gerold syndrome. In addition to several premature aging features, RTS patients are characterized by aneuploidy involving either loss or gain a single chromosome. Chromosome mosaicism isochromosomes chromosomes 2, 7, 8 have been reported RecQL4-deficient patients, but precise role...

Porphyrin-based catalytic oxidation is one of the most representative biomimetic catalysis. To mimic nature, a positive charged porous membrane, quaternized polysulfone (QPSf) membrane with spongelike structure, was prepared for supporting meso-tetraphenylsulfonato porphyrin (TPPS). The influence polymer concentration, coagulation bath, and additives on structure substrate explored, optimized porosity 87.1% water flux 371 L·m–2·h–1 at 0.1 MPa obtained. Monolayer TPPS adsorbed QPSf surface by...

Abstract The dynamic interfacial tension (IFT) of ethoxylated fatty acid methyl ester solutions against n ‐alkanes, kerosene, and diluted heavy oil have been investigated by spinning drop tensiometry. influences ethylene oxide (EO) groups alkyl chain length on IFT were investigated. experiment results show that the water solubility decreases with an increase in or a decrease EO groups. ability to lower hydrocarbons improves both increasing group at best hydrophilic‐lipophilic balance, which...

Abstract Ubiquitination-dependent DNA damage response (DDR) signals play a critical role in the cellular choice of repair pathways. Human helicase RecQL4 participates replication and repair, loss is associated with autosomal recessive genetic disorders characterized by genomic instability features. In an earlier study, was isolated as stable complex that contained two ubiquitin ligases N-end rule (UBR1 UBR2). However, it unknown whether or not ubiquitination status for its function. Here, we...

RecQL4, one of the 5 human RecQ helicases, is a key mediator genomic stability and its deficiency can cause premature aging phenotypes. Here, by using CRISPR/Cas RNAi technology, we demonstrated that autophagy level was elevated in both RecQL4 knockdown knockout cells compared with those control cells. Surprisingly, mitochondrial content increased LC3 co-localization mitochondria partially lost cells, suggesting impaired mitophagic processes U2OS Furthermore, found destabilized PINK1. In...

At present, the essence of traditional methods for determination harmonic responsibility among different parts in power system is mainly based on contribution relevant physical quantity point common coupling (PCC) both utility side and customer side. However, theoretical basis various much difference, leading to a large difference final calculation result responsibility. Therefore, there no reliable approach applied actual cases. This project aims fill current gap literature, proposing new...

Background Both in vitro and vivo data have demonstrated the TGFBI gene functions as a putative tumor suppressor is frequently downregulated human tumors of different histological types. The hypermethylation promoter, one main regulatory mechanisms, associated with silencing. In this study, we used methylation-specific PCR (MSP) method to evaluate methylation status promoter leukemias. Methods Real-time RT-PCR approaches were performed define expression leukemia cell lines clinical samples....

The dilational properties of demulsifiers PE1 and PE2 at the decane-water interface were investigated. Meanwhile, influence on interfacial modulus 5% crude oil also was explored. experimental results indicate that diffusion process is still not main factors to control film even large concentration. demulsifier passes through a maximum with increasing concentration, changes little concentration interface. moduli inerfaces decrease addition both demulsifiers. At low effect reducing stronger...

ABSTRACT Dilational rheology method was used to study the interfacial properties of Branch‐Preformed Particle Gel (PPG) and its interaction with Shengli crude oil fractions at kerosene/water interface. The results showed that dilational modulus increased monotonously increasing PPG concentration, high values could be due formation multilayer near Study on between components different have interactions molecules oil/water acidic can adsorb onto interface form mixed adsorption film by...

MMS19 localizes to the cytoplasmic and nuclear compartments involved in transcription nucleotide excision repair (NER). However, whether mitochondria, where it plays a role maintaining mitochondrial genome stability, remains unknown. In this study, we provide first evidence that is localized inner membrane of mitochondria participates mtDNA oxidative damage repair. knockdown led dysfunctions including decreased copy number, diminished capacity, elevated levels common deletion after stress....

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NeuroD, a basic helix–loop–helix transcription factor, is capable of converting embryonic epidermal cells into neuronal cells. However, whether histone deacetylases (HDACs) are involved in the autoregulation neuroD or not unclear. In this study, expression was found to be significantly increased all-trans retinoid acid-treated P19 Meanwhile, could itself enhance its promoter activity and mRNA expression. By using specific inhibitors modification enzymes, HDAC3 identified specifically augment...

Abstract C 18 H 14 S 2 , monoclinic, P 1 / c (no. 14), a = 10.196(3) Å, b 14.756(4) 9.508(3) β 103.965(14)°, V 1388.3(7) Å 3 Z 4, R gt ( F ) 0.0480, wR ref 0.1207, T 200(2) K.