ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMultiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA DetectionSatoshi. Takahashi, Katsuhiko. Murakami, Takashi. Anazawa, and Hideki. KambaraCite this: Anal. Chem. 1994, 66, 7, 1021–1026Publication Date (Print):April 1, 1994Publication History Published online1 May 2002Published inissue 1 April 1994https://pubs.acs.org/doi/10.1021/ac00079a016https://doi.org/10.1021/ac00079a016research-articleACS PublicationsRequest...

To slow the translocation of single-stranded DNA (ssDNA) through a solid-state nanopore, nanopore was narrowed, and effect narrowing on speed investigated. In order to accurately measure speed, long (5.3 kb) ssDNA (namely, ss-poly(dA)) with uniform length (±0.4 synthesized. The diameters nanopores fabricated by transmission electron microscope were controlled atomic-layer deposition. Reducing diameter from 4.5 2.3 nm slowed down more than 16 times (to 0.18 μs base(-1)) when 300 mV applied...

A very simple and highly sensitive capillary array gel electrophoresis system is constructed to analyze DNA fragments. On-column detection of migration in a large number gel-filled capillaries carried out using side-entry laser irradiation with CCD camera, although it has been considered impossible because the scattered strongly at surfaces first few capillaries. By optimizing optical conditions, beam can be focused repeatedly irradiate all held on plate by working each as cylindrical convex...

In spectral imaging, an optical system generates two mutually dependent kinds of crosstalk on image sensor: (i.e., mixing) between fluorescences different dyes and spatial artifacts) from emission points in the field view sensor. Therefore, algorithm to cancel both simultaneously simultaneous unmixing image-artifact reduction) is proposed. The based assumption that a matrix point-spread functions, PSFs) consisting all ratios constant regardless causes crosstalk. By applying...

We have developed a novel high-performance quantitative assay for unamplified nucleic acids that is based on single-molecule imaging. The apparatus simple but highly sensitive detection system uses normal CCD camera instead of an image-intensified camera. After the DNA molecules in sample were labeled with YOYO-1, they induced to migrate electrophoretically polymer solution and imaged. No chemical or biochemical amplification was required. Direct quantitation by counting possible, because...

Abstract One of the challenges in liquid biopsy for early cancer detection is ascribed to fact that mutation DNA often represents an extremely small ratio less than 1% compared wild-type genes blood. However, conventional fragment analysis with capillary electrophoresis (CE), detectable allele frequency could be about 5%. In this work, we developed original reagent-based single base extension (SBE) reactions cancer-associated assay using a commercially available CE device, and investigated...

We have developed a simple and high-throughput capillary-array electrophoresis system that uses side-entry on-column laser irradiation. The number of capillaries in an array is generally limited by laser-power attenuation along the due to reflection divergence. overcame these problems placing water adding glass rod lenses between capillaries. As result, up 45 could be simultaneously irradiated with single beam fluorescence from all detected high sensitivity. demonstrated throughput 12 kbp/h...

This paper reports our current development of "Robo-ducky", the rice field weeding robots, which is inspired from farming with natural ducks. presents (1) improvement motion ability robot by modifying size and (2) investigation damage put to plants in an agricultural scientific way such as counting up number seeds soil defect according weeding. These investigations show has enough actual fields.

A five-color fluorescence-detection system for eight-channel plastic-microchip electrophoresis was developed. In the eight channels (with effective electrophoretic lengths of 10 cm), single-stranded DNA fragments were separated single-base resolution up to 300 bases within min), and seventeen-loci STR genotyping forensic human identification successfully demonstrated. system, a side-entry laser beam is passed through (eight channels), with alternately arrayed seven sacrificial (seven B by...

Although multi-point, multi-color fluorescence-detection systems are widely used in various sciences, they would find wider applications if miniaturized. Accordingly, an ultra-small, four-emission-point and four-color system was developed. Its size (space between emission points a detection plane) is 15 × 10 12 mm, which three-orders-of-magnitude smaller than that of conventional system. Fluorescence from four with interval 1 mm on the same plane respectively collimated by lenses split into...

A novel side-gated ultrathin-channel nanopore FET (SGNAFET), for fast and label-free DNA sequencing with high resolution sensitivity, is proposed. The goal of the SGNAFET to identify four types nucleotides in by changes channel current SGNAFET. Aiming reach that goal, a thickness (t <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">ch.</sub> ) 2 or 4 nm was successfully operated could detect translocations through its on basis current.

An ultra-small (54 × 58 8.5 mm) and large aperture (1 7 nine-color spectrometer-using an array of ten dichroic mirrors "biparted" as two layers-was developed used for snapshot spectral imaging. Incident-light flux with a cross section smaller than the size is split into nine color fluxes 20-nm-width contiguous wavelength bands central wavelengths 530, 550, 570, 590, 610, 630, 650, 670, 690 nm. Images are simultaneously efficiently measured by image sensor. Unlike conventional dichroic-mirror...

We studied the fundamental instrumental issues relevant to a capillary-based integrated system measure expression of specific gene directly from cells. Samples were introduced into capillary by use syringe pump. All reactions carried out in microthermocycler, where part having 1 μL inner volume was used as reaction vessel. First, cells lysed heating release RNA, followed deoxyribonuclease (DNase) treatment. Then, reverse transcription-polymerase chain (RT-PCR) performed obtain amplified...

A dual-view imaging system for simultaneous four-color single-molecule (SM) detection was developed. As the procedure, four species of SM fluorophores, namely, Alexa 488, 555, 647, and 680, are immobilized on different slides excited by evanescent-wave illumination. Fluorescence emitted from an fluorophore is split a wide-range dichroic mirror (WR DM) in optics imaged as two fluorescence spots (SM spots) electron-multiplying charge-coupled device (EM-CCD) at 100 Hz. The transmittance WR DM...

Mutant (MT) in a large excess of wild type (WT) was quantified with high-sensitivity (LOD 0.004% MT/WT) and four-orders-of-magnitude dynamic range (0.01–100% by high-dynamic-range capillary-array electrophoresis (HiDy CE).

An ultra-small and highly efficient spectral-detection system for four emission points was developed by integrating an injection-molded-plastic four-lens array, a seven-dichroic-mirror image sensor as one device. The array further miniaturized compared to our previous four-dichroic-mirror measures including reduction of the thickness each dichroic mirror from 1.0 0.5 mm. As result, enables sensitive low-crosstalk seven-color detection laser-induced fluorescence four-capillary array. This...

A side-gated, ultrathin-channel nanopore FET (SGNAFET) is proposed for fast and label-free DNA sequencing. The concept of the SGNAFET comprises detection changes in channel current during translocation through a identifying four types nucleotides as result these changes. To achieve this goal, both p- n-type SGNAFETs with thicknesses 2 or 4 nm were fabricated, stable transistor operation air, water, KCl buffer solution confirmed. In addition, synchronized observed between ionic SGNAFET's...

We developed a total-internal-reflection (TIR) fluorescence microscopy using three dichroic mirrors and four charge-coupled devices (CCDs) to detect simultaneously colors of single-molecule (SM) fluorophores. Four spectrally distinct species fluorophores (Alexa 488, Cy3, Cy5, or Cy5.5) were each immobilized on different fused silica slide. A the slide was irradiated simultaneously, by two excitation beams from an Ar ion laser (488 514.5 nm) diode (642 through TIR surface. Fluorescence...

A simple and highly sensitive technique for laser-induced fluorescence detection on multiple channels in a plastic microchip was developed, its effectiveness demonstrated by laser-beam ray-trace simulations experiments. In the microchip, with refractive index nC, B are arrayed alternately respectively filled materials indexes nA electrophoresis analysis nB control. It shown that laser beam entering from side of channel array traveled straight irradiated all simultaneously effectively because...

We found a way to increase the precision with which biomolecules present at concentrations below 10(-10) M can be quantified by fluorescence correlation spectroscopy (FCS). The effectiveness of was demonstrated experimentally using single-element aspheric objective lens, newly developed reduce cost FCS instruments. In first part this paper, relative standard deviation (RSD) FCS-based concentration measurements is estimated theoretically an analytical approximation assuming detection volume...

We developed a new kind of capillary array for electrophoresis by using the numerical-control (NC) wiring technique conventionally used to produce printed-circuit boards. Laminating two polyimide sheets after laying cylindrical capillaries between them according designed geometries, we fabricated 16-lane laminated (LCA) 9.9 cm long, 7.2 wide, and 0.5 mm thick in which effective length all was only 10.9 cm. This compact LCA thus had separation columns as short those chips lithography...