Raman spectra are molecular structure-specific and hence employed in applications requiring chemical identification. The advent of efficient handheld smartphone-based instruments is promoting widespread the technique, which often involve less trained end users. Software modules that enable spectral library searches based on pattern matching an essential part such applications. spectrum recorded by users will naturally have varying levels signal to noise (SN), baseline fluctuations, etc.,...

Janus structures have attracted a great deal of interest because their fascinating properties and potential for applications. In this study, we demonstrate that hyperbranched polymers, bearing randomly placed docosyl (C22 alkyl segment) PEG segments on periphery, can readily reconfigure so as to segregate the segments, thereby generating Janus-type termed hybramers. DSC studies clearly reveal an endothermic transition corresponds melting domains, while Langmuir isotherms these polymers form...

Using Raman and Mid-Infrared (MIR) spectroscopic imaging techniques one can examine the spatial distribution of various molecular constituents in a heterogeneous sample at microscopic scale. MIR spectroscopy provide bond-specific vibrational frequencies to characterize species without external labelling. In addition, these are rapid, non-invasive multiplex advantage. this review we discuss instrumentation, applications latest advancements fields, ranging from materials science medicine...

Multifocus Raman imaging is one of the fast-imaging alternatives to conventional single point mapping technique.

Abstract Raman imaging has become an attractive technology in molecular biology because of its ability to detect multiple components simultaneously without labeling. Two major limitations accurately accounting for spectral features, viz ., background removal and unmixing, have been overcome by employing a modified effective routine multivariate curve resolution (MCR). With our improved strategy, we spectrally isolated seven structurally specific biomolecules any post-acquisition treatments....

Raman microspectroscopy is a minimally invasive technique that can identify molecules without labeling. In this study, we demonstrate the detection of penicillin G inside Penicillium chrysogenum KF425 fungal cells. spectra acquired from cells had highly overlapped spectroscopic signatures and hence were analyzed with multivariate curve resolution by alternating least-squares (MCR-ALS) to extract individual molecular constituents. addition detecting spatial distribution multiple constituents...

We have recently demonstrated a methodology to estimate the percent crystallinity (PC) of polymers directly with Raman spectroscopy and multivariate curve resolution (MCR) by alternating least-squares (ALS). In MCR-ALS methodology, spectrum semicrystalline polymer is separated into two constituent components (crystalline molten/amorphous) their corresponding concentrations. The necessitates that at any temperature be linear combination MCR spectral (one molten one crystalline). This true in...

Condensation of water droplets during rapid evaporation a polymer solution, under humid conditions, has been known to generate uniformly porous films. Similar films are also formed when solution the in THF containing small amounts water, is allowed evaporate rapidly air flow; this suggests that may be final stages film formation. In presence added surfactants, interface could become lined with surfactants and consequently internal walls pores generated, upon removal decorated hydrophilic...

We demonstrate a methodology to estimate the percent crystallinity of polymers directly with Raman spectroscopy and multivariate curve resolution (MCR) by alternating least-squares (ALS). In this methodology, spectrum semicrystalline polymer is separated into two constituent components (crystalline molten) their corresponding concentrations. The can be estimated as change in area intensity molten spectral-component when cools from temperature above melting point room temperature. number...

The mechanism of production extracellular vesicles (EVs) and their molecular contents are great interest due to diverse roles in biological systems far from being completely understood. Even though cellular cargo releases mediated by EVs have been demonstrated several cases, role secondary metabolite release remains elusive. In this study, aspect is investigated detail using Raman microspectroscopic imaging. Considerable evidence provided suggest that the antibiotic penicillin filamentous...

Abstract Spatial transcriptome analysis of formalin-fixed paraffin-embedded (FFPE) tissues using RNA-sequencing (RNA-seq) provides interactive information on morphology and gene expression, which is useful for clinical applications. However, despite the advantages long-term storage at room temperature, FFPE may be severely damaged by methylene crosslinking provide less than fresh-frozen tissues. In this study, we proposed a sensitive micro-tissue RNA-seq method that combines punching tissue...

Methodologies for direct intracellular imaging of RNA and DNA are necessary the advancement bioimaging. Here we show label-free in single cells by isolating their accurate Raman spectra. images from interphase intact nucleus, while those mitotic reveal condensed chromosome. The chromosome enough to assign stage cell division (e.g., metaphase). spectral features indicate B-DNA double helical conformational form all lines investigated here. RNAs, on other hand, liquid-liquid phase separated...

Single-molecule force spectroscopy has proven to be an efficient tool for the quantitative characterization of flexible foldamers on single-molecule level in this study. The extent folding been estimated quantitatively first time best our knowledge, which is crucial a better understanding "folding-process" level. Therefore, study may provide guidance regulate realizing rational control over functions bulk materials.

A peripherally clickable hyperbranched polyester carrying numerous propargyl terminal groups was prepared by a simple melt transesterification polycondensation of suitably designed AB(2) monomer; this hyperscaffold then transformed into variety different derivatives using the Cu-catalyzed azide-yne click reaction. Functionalization periphery with equimolar quantities mutually immiscible segments, such as hydrocarbon, fluorocarbon, and PEG, yielded frustrated molecular systems that readapt...

A low-generation amphiphilic dendrimer is applied as a nano-reactor for polymerization the first time.

Label-free quantitative phase imaging can potentially measure cellular dynamics with minimal perturbation, motivating efforts to develop faster and more sensitive instrumentation. We characterize fast, single-shot gradient microscopy (ss-QPGM) that simultaneously acquires multiple polarization components required reconstruct images. integrate a computationally efficient least squares algorithm provide real-time, video-rate (up

Raman imaging has transcended in recent times from being an analytical tool to a molecular profiling technique. Biomedical applications of this technique often rely on singular-value decomposition (SVD), principal component analysis (PCA), etc. for data analysis. These methods, however, obliterate the information contained original leading speculative interpretations based relative intensities. In present study, SVD images Penicillium chrysogenum resulted 11 spectral components and...

Rickettsiales-like organisms are important for the survival and functioning of corals, prompting an investigation their complete genomes. Earlier reports genomes these remain incomplete. Here, we report a novel draft genome Rickettsiales bacterial strain SESOKO1, found in Acropora tenuis coral, using single-cell technology.