A5046368790- Spectroscopy Techniques in Biomedical and Chemical Research
- Optical Imaging and Spectroscopy Techniques
- Spectroscopy and Chemometric Analyses
- Orbital Angular Momentum in Optics
- Digital Holography and Microscopy
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Photoacoustic and Ultrasonic Imaging
- Crystallography and molecular interactions
- Microfluidic and Bio-sensing Technologies
- Photoreceptor and optogenetics research
- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Dental Implant Techniques and Outcomes
- Random lasers and scattering media
- Advanced Fluorescence Microscopy Techniques
- Oral Health Pathology and Treatment
- Molecular Biology Techniques and Applications
- thermodynamics and calorimetric analyses
- Non-Invasive Vital Sign Monitoring
- Optical Coherence Tomography Applications
- Neonatal Health and Biochemistry
- Lipid Membrane Structure and Behavior
- Computational Drug Discovery Methods
- Ultrasound and Hyperthermia Applications
Vanderbilt University
2019-2023
University College Cork
2022-2023
Indian Institute of Science Bangalore
2010-2022
San Francisco State University
2016-2020
University of Alabama at Birmingham
2018
Raman and Infrared (IR) spectroscopies provide information about the structure, functional groups environment of molecules in sample. In combination with a microscope, these techniques can also be used to study molecular distributions heterogeneous samples. Over past few decades IR microspectroscopy based have been extensively understand fundamental biology responses living systems under diverse physiological pathological conditions. The spectra from biological are complex diverse, owing...
It is commonly thought that biological media cannot exhibit an appreciable nonlinear optical response. We demonstrate, for the first time to our knowledge, a tunable nonlinearity in suspensions of cyanobacteria leads robust propagation and strong self-action light beam. By deliberately altering host environment marine bacteria, we show experimentally interaction can result either deep penetration or enhanced scattering through bacterial suspension, while viability cells remains intact. A...
Osmotic conditions play an important role in the cell properties of human red blood cells (RBCs), which are crucial for pathological analysis some diseases such as malaria. Over past decades, numerous efforts have mainly focused on study RBC biomechanical that arise from unique deformability erythrocytes. Here, we demonstrate nonlinear optical effects RBCs suspended different osmotic solutions. Specifically, observe self-trapping and scattering-resistant propagation a laser beam through...
Background: Myocardial infarction (MI) induces an intense injury response that ultimately generates a collagen-dominated scar. Although required to prevent ventricular rupture, the fibrotic process is often sustained in manner detrimental optimal recovery. Cardiac myofibroblasts are cells tasked with depositing and remodeling collagen prime target limit after MI. Serotonin 2B receptor (5-HT ) signaling has been shown be harmful variety of cardiopulmonary pathologies could play important role...
Oral potentially malignant disorders (OPMD) serve as early indicators of oral cancer. These conditions require reliable, non-invasive and real-time diagnostic methods for effective detection screening. Saliva, an easily accessible biofluid, has emerged a promising tool to detect OPMD-associated biomarkers. This proof-of-concept study investigated the application suspended-core optofluidic photonic crystal fibers (PCF) Raman spectroscopy distinguish between saliva from healthy controls OPMD...
Oxidative stress due to excessive accumulation of reactive oxygen or nitrogen species in the brain as seen certain neurodegenerative diseases can have deleterious effects on neurons. Hydrogen peroxide, endogenously generated neurons under normal physiological conditions, produce an excess hydroxyl radical via a Fenton mediated mechanism. This may induce acute oxidative injury if not scavenged removed effectively by antioxidants. There are several biochemical assay methods estimate cells;...
Myopathies are among the major causes of mortality in world. There is no complete cure for this heterogeneous group diseases, but a sensitive, specific, and fast diagnostic tool may improve therapy effectiveness. In study, Raman spectroscopy applied to discriminate between muscle mutants Drosophila on basis associated changes at molecular level. spectra were collected from indirect flight muscles mutants, upheld(1) (up(1)), heldup(2) (hdp(2)), myosin heavy chain(7) (Mhc(7)), actin88F(KM88)...
The therapeutic efficacy and safety of stored red blood cells (RBCs) relies on minimal in-bag hemolysis. accuracy current methods measuring hemolysis can suffer as a result specimen collection processing artefacts.To test whether Raman spectroscopy could be used to assess hemolysis.RBCs were for long 42 days. spectra RBCs measured before after washing, was in supernatant by visible spectroscopy.Raman indicated increased concentrations oxyhemoglobin (oxyHb) methemoglobin (metHb), decreased...
Early diagnosis of oral cancer is critical to improve the survival rate patients.
Using Raman and Mid-Infrared (MIR) spectroscopic imaging techniques one can examine the spatial distribution of various molecular constituents in a heterogeneous sample at microscopic scale. MIR spectroscopy provide bond-specific vibrational frequencies to characterize species without external labelling. In addition, these are rapid, non-invasive multiplex advantage. this review we discuss instrumentation, applications latest advancements fields, ranging from materials science medicine...
Optical trapping is important in assessing the deformability of red blood cells (RBCs), providing quick, noncontact force measurement liquid medium with piconewton resolution. Traditional techniques, though, can involve complicated processes or cause photodamage. This study employs ``tug-of-war'' (TOW) optical tweezers object-adapted potentials, to simply and stably trap, stretch, squeeze a single RBC under different osmotic conditions, thus enabling assessment deformability. The large...
Acetaminophen is a widely prescribed drug used to relieve pain and fever; however, it leading cause of drug-induced liver injury burden on public healthcare. In this study, hepatotoxicity in mice post oral dosing acetaminophen was investigated using sera samples with Fourier Transform Infrared microspectroscopy. The infrared spectra treated livers BALB/c show decrease glycogen, increase amounts cholesteryl esters DNA respectively. Rescue experiments L-methionine demonstrate that depletion...
Establishing the precise timeline of a crime can be challenging as current analytical techniques used suffer from many limitations and are destructive to body fluids encountered at scenes. Raman spectroscopy has demonstrated excellent potential in forensic science it provides direct information about structural molecular changes without need for processing or extracting samples. However, its applicability is limited pure fluids, signals substrate underlying these greatly influence models age...
In the past decade, development of artificial materials exhibiting novel optical properties has become a major scientific endeavor. One particularly interesting system is synthetic soft matter, which plays central role in numerous fields ranging from life sciences, chemistry to condensed matter and biophysics. this paper, we review briefly force-induced nonlinearities colloidal suspensions, can give rise nonlinear self-trapping light for enhanced propagation through otherwise highly...
The fracture resistance of bone arises from the hierarchical arrangement minerals, collagen fibrils (
Rapid advancement of novel optical spectroscopy and imaging systems relies on the availability well-characterised reproducible protocols for phantoms as a standard validation technique. The tissue-mimicking are also used to investigate photon transport in biological samples before clinical trials that require well-characterized with known properties (reduced scattering (μ's) absorption (μa) coefficients). However, at present, there is limited literature available providing phantom recipes...
Phantoms simultaneously mimicking anatomical and optical properties of real tissues can play a pivotal role for improving dosimetry algorithms. The aim the paper is to design develop hybrid phantom model that builds up on strengths solid liquid phantoms various structures prostate cancer photodynamic therapy (PDT) validation. comprises photosensitizer-embedded gelatin lesion within Intralipid shape surrounded by silicone outer shell. was well characterized properties. final assembled also...
Sepsis is a life threatening condition resulting from high burden of infection. It major health care problem and associated with inflammation, organ dysfunction significant mortality. However, proper understanding delineating the changes that occur during this complex remains challenge. A comparative study involving intra-peritoneal injection BALB/c mice Salmonella Typhimurium (infection), lipopolysaccharide (endotoxic shock) or thioglycollate (sterile peritonitis) was performed. The in...
Abstract The ability to characterize the combined structural, functional, and thermal properties of biophysically dynamic samples is needed address critical questions related tissue structure, physiological dynamics, disease progression. Towards this, we have developed an imaging platform that enables multiple nonlinear modalities be with on a common sample. Here demonstrate label-free multimodal live cells, excised tissues, rodent brain models. While potential applications this technology...
A method to acquire the Raman spectra of sub-surface components using diffusely focused radiation in a microscope sampling configuration is described.