A5044903529- Reproductive System and Pregnancy
- Preterm Birth and Chorioamnionitis
- Endometriosis Research and Treatment
- Neonatal Respiratory Health Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Neuroendocrine regulation and behavior
- Reproductive Physiology in Livestock
- Uterine Myomas and Treatments
- Intestinal and Peritoneal Adhesions
- Endometrial and Cervical Cancer Treatments
- Reproductive Biology and Fertility
- Pregnancy and preeclampsia studies
- Maternal and fetal healthcare
- Pregnancy-related medical research
- Infant Nutrition and Health
- Nanoplatforms for cancer theranostics
- Infant Development and Preterm Care
- Spectroscopy and Chemometric Analyses
- COVID-19 Impact on Reproduction
- Maternal and Perinatal Health Interventions
- Education, Leadership, and Health Research
- Bee Products Chemical Analysis
- Optical Imaging and Spectroscopy Techniques
- Linguistics and language evolution
- Congenital Heart Disease Studies
Vanderbilt University
2012-2024
Vanderbilt University Medical Center
2014-2024
Nashville Oncology Associates
2018
University of California, Davis
2011
Southern Illinois University School of Medicine
2006-2009
Endometriosis is a common, complex gynecologic disorder characterized by the presence of endometrial glands and stroma at extrauterine (ectopic) sites. In women who develop this disease, alterations in specific biological processes involving both endocrine immune systems have been observed, which may explain survival growth displaced tissue affected women. past decade, considerable amount research has implicated role for progesterone action eutopic ectopic sites contribute to excessive...
A probe-based Raman spectroscopy system using a single laser module with two excitation wavelengths has been developed for measuring the fingerprint and high wavenumber regions detector.
Uterine natural killer (uNK) cells are the most abundant lymphocytes in uterus during early pregnancy and play a role spiral arteriole modifications. In present study, we investigated whether uNK cell populations differed between mouse decidua deciduoma. Histochemical staining using Dolichos biflorus agglutinin (DBA) lectin was used to identify classify their stages of maturation. We found differences pattern localization density deciduoma at Days 2-4 after onset decidualization. The were...
β-catenin plays a role in cell adhesion and as transcriptional coactivator. Its levels are regulated cells by controlling its degradation through ubiquitination two different E3 ligase complexes. One complex contains β-transducing repeat containing (BTRC) protein, which binds to when phosphorylated on specific (S33 S37) residues, whereas the other involves calcyclin-binding protein (CACYBP). The aim of this study was determine localization total active (S33/S37-dephosphorylated) pregnant...
Abstract The purpose of this study was to determine whether the conceptus directs formation a tight- and adherens-dependent permeability barrier formed by primary decidual zone normal progression cell differentiation during embryo implantation. Four artificial models decidualization were used, some apparently more physiological than others. results show that both three quite different from pregnant uteri. One model decidualization, namely pseudopregnant animals receiving concanavalin...
Monitoring cervical structure and composition during pregnancy has high potential for prediction of preterm birth (PTB), a problem affecting 15 million newborns annually. We use in vivo Raman spectroscopy, label-free, light-based method that provides molecular fingerprint to non-invasively investigate normal impaired remodeling. Prostaglandins stimulate uterine contractions are clinically used ripening pregnancy. Deletion cyclooxygenase-1 (Cox-1), an enzyme involved production these...
Excessive cytokine inflammatory response due to chronic or superphysiological level of microbial infection during pregnancy leads complications such as early defects/loss and preterm birth. Bacterial toxin lipopolysaccharide (LPS), long recognized a potent proinflammatory mediator, has been identified risk factor for complications. Alkaline phosphatase (AP) isozymes have shown detoxify LPS by dephosphorylation. In this study, we examined the role alkaline in mitigating LPS-induced mice. We...
The uterine myometrium (UT-myo) is a therapeutic target for preterm labor, labor induction, and postpartum hemorrhage. Stimulation of intracellular Ca2+-release in UT-myo cells by oxytocin final pathway controlling myometrial contractions. goal this study was to develop dual-addition assay high-throughput screening small molecular compounds, which could regulate Ca2+-mobilization cells, hence, Primary murine 384-well plates were loaded with Ca2+-sensitive fluorescent probe, then screened...
Within the mouse endometrium, secreted phosphoprotein 1 ( SPP1 ) gene expression is mainly expressed in luminal epithelium and some macrophages around onset of implantation. However, during progression decidualization, it mesometrial decidua. To date, precise cell types responsible for decidua has not been absolutely identified. The goal present study was to assess uteri pregnant mice (decidua) decidualization compared with those undergoing artificially induced (deciduoma). Significantly P...
Cyclooxygenase (COX)-derived prostaglandins stimulate uterine contractions and prepare the cervix for parturition. Prior reports suggest Cox-1 knockout (KO) mice exhibit delayed parturition due to impaired luteolysis, yet mechanism late-onset delivery remains unclear. Here, we examined key factors normal onset of determine whether any could account phenotype. Pregnant Cox-1KO did not display altered timing embryo implantation or postimplantation growth. Although messenger RNAs...
Fluorescence imaging is a well-established optical modality that has been used to localize and track fluorophores in vivo demonstrated great potential for surgical guidance. Despite the variety of currently being researched, many existing intraoperative fluorescence systems are specifically designed limited number applications. We present modular wide-field overlay tissue system guidance comprised commercially available standardized components. Its layout allows accommodation broad range...
In mouse models used to study parturition or pre-clinical therapeutic testing, measurement of uterine contractions is limited either ex vivo isometric tension operative intrauterine pressure (IUP). The goal this was to: (1) develop a method for transcervical insertion catheter measure in contractile during pregnancy, (2) determine whether can be utilized numerous times single pregnancy without affecting the timing delivery fetal outcome and (3) compare activity between term preterm labor...
Preterm birth and postpartum hemorrhage are the leading causes of neonatal maternal morbidities worldwide, respectively. Current clinically utilized tocolytics uterotonics to manage these obstetric conditions limited due their off-target effects and/or lack efficacy. Thus, an ideal tocolytic or uterotonic would be uterine-selective with rapid onset long-duration Here, we discuss strategies for discovery new therapeutic targets compounds that regulate uterine contractility aforementioned properties.
Abstract A great need exists to develop tocolytic and uterotonic drugs that combat poor, labor-related maternal fetal outcomes. widely utilized method assess novel compounds for their efficacy is the isometric organ bath contractility assay. Unfortunately, water-insoluble can be difficult test using physiological, buffer-based, Common methods overcoming solubility issues include solvent variation, cosolvency, surfactant or complexion use, emulsification. However, these options drug delivery...