A5041475133- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Microbial Inactivation Methods
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Photoreceptor and optogenetics research
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- bioluminescence and chemiluminescence research
- Marine Biology and Ecology Research
- Spaceflight effects on biology
- Wastewater Treatment and Nitrogen Removal
- Enzyme Production and Characterization
- Tardigrade Biology and Ecology
- Quantum, superfluid, helium dynamics
- Marine Bivalve and Aquaculture Studies
- Hemoglobin structure and function
- Atomic and Subatomic Physics Research
- Cephalopods and Marine Biology
- RNA and protein synthesis mechanisms
- Magnetic and Electromagnetic Effects
- Isotope Analysis in Ecology
- Marine Invertebrate Physiology and Ecology
Japan Agency for Marine-Earth Science and Technology
2012-2021
National Medical Research Center of Dentistry and Maxillofacial Surgery
2021
Mie University
2021
Nagoya City University
2020
Osaka City University
2007-2013
Kobe University
2012
Tokyo Institute of Technology
2002-2011
The Japanese Society of Gastroenterological Surgery
2010-2011
Japan Atomic Energy Agency
2011
Okayama University of Science
2008
Two strains of obligately barophilic bacteria were isolated from a sample the world's deepest sediment, which was obtained by unmanned deep-sea submersible Kaiko in Mariana Trench, Challenger Deep, at depth 10,898 m. From results phylogenetic analysis based on 16S rRNA gene sequences, DNA-DNA relatedness study, and fatty acid composition, first strain (DB21MT-2) appears to be most highly similar Shewanella benthica close relatives, second (DB21MT-5) closely related genus Moritella. The...
The genus Shewanella is one of the typical deep-sea bacterial genera. Two isolated species, benthica and violacea, were found to be able grow better under high hydrostatic pressure conditions than at atmospheric pressure. These species are not only piezophilic (barophilic), but also psychrophilic. Many psychrophilic psychrotolerant have been characterized from cold environments, such as seawater in Antarctica or North Sea. Some these cold-adapted shown piezotolerant, meaning that growth...
A novel marine bacterium, strain JCS350(T), was isolated from sediment samples collected a cold-seep area. The 16S rRNA gene sequence of the isolate showed high similarity to that Erythrobacter luteolus SW-109(T) (95.9 % similarity). Lower similarities were shown other members genus (94.6-95.4 %) and Porphyrobacter (94.5-95.2 %). Phylogenetic analysis with all family Erythrobacteraceae several Sphingomonadaceae revealed formed phyletic line [Erythrobacter] distinct Erythrobacteraceae....
Two strains of obligately piezophilic bacteria were isolated from sediment collected the bottom surface a small canyon on seaward slope Japan Trench at depth 6278 m. The strains, Y223GT and Y251E, are closely affiliated with members genus Colwellia basis 16S rRNA gene sequence analysis. G + C contents both about 39 mol%. DNA-DNA hybridization values between these reference significantly lower than those accepted as phylogenetic definition species. novel Gram-negative, polarly flagellated...
Strains 2674T (=LMG 21259T =JCM 11435T) and 2693T 21258T 11436T) were isolated from Atlantic sediments at a temperature of 2 degrees C depth 2815 m off the West African coast. Polyphasic evidence indicates that two strains belong to genus Moritella represent distinct species, for which names profunda sp. nov. (for strain 2674T) abyssi 2693T) are proposed. The moderate piezophily organisms is intermediate between type marina, not piezophilic, yayanosii, an obligate piezophile. Both strict...
The nucleotide sequence of the Streptococcus mutans GS-5 gtfD gene coding for glucosyltransferase which synthesizes water-soluble glucan (GTF-S) has been determined. complete contains 4293 base pairs and unprocessed protein is composed 1430 amino acids with a molecular mass 159814 Da. terminus resembles signal sequences other extracellular proteins secreted by S. that GTF-I downei. In addition, GTF-S exhibits high acid similarity strain enzymes responsible insoluble synthesis (GTF-I, GTF-SI)...
Some rod-shaped bacteria, including Escherichia coli , exhibit cell filamentation without septum formation under high-hydrostatic-pressure conditions, indicating that the cell-division process is affected by hydrostatic pressure. The effects of elevated pressure on FtsZ-ring in E. cells were examined using indirect immunofluorescence microscopy. Elevated 40 MPa completely inhibited colony cultivation conditions used, and exhibited obviously filamentous shapes. In elongated cells, normal...
We have isolated thirteen different bacterial strains as poly ε-caprolactone (PCL)-degrading bacteria from the Kurile and Japan Trenches at a depth of 5,000-7,000 m (deeper ocean bottoms). The isolates belong to Shewanella, Moritella, Psychrobacter Pseudomonas genera. This is first record PCL degrading deep-sea environments over 5,000 m. Six isolates, numbered CT01 in genus CT12, JT01 JT04 JT05 Psychrobacter, JT08 were selected for investigation their cell shapes, abilities several aliphatic...
A new anaerobic, thermophilic, CO-utilizing marine bacterium, strain JMT, was isolated from a submarine hot vent in Okinawa Trough. Cells of JMT were non-motile thin straight rods, sometimes branching, with cell wall the Gram-positive type, surrounded an S-layer. Chains three to five cells often observed. The isolate grew chemolithotrophically on CO, producing equimolar quantities H2 and CO2 (according equation CO+H2O-->CO2+H2) organotrophically peptone, yeast extract, starch, cellobiose,...
A sample of deep-sea sediment was obtained from the Japan Trench at a depth 6292 m using pressure-retaining sampler. Microorganisms in were cultivated marine broth 2216 ambient pressure (65 MPa) without decompression, and atmospheric (0.1 as control experiment. 16S ribosomal RNA genes (rDNA) amplified by PCR DNA extracted original mixed cultures, nucleotide sequences determined. The results phylogenetic analysis based on rDNA indicated that microbial diversity samples showed wide...
Summary A depth profile of naturally occurring bacterial community structures associated with the deep‐sea cold seep push‐core sediment in Japan Trench at a 5343 m were evaluated using molecular phylogenetic analyses RNA reverse transcription‐PCR (RT‐PCR) amplified 16S crDNA fragments. total 137 clones (complimentary rDNA) phylotypes (phylogenetic types) obtained three different depths (2–4, 8–10 and 14–16 cm) identified partial sequencings. from dominantly composed δ‐ ɛ‐Proteobacteria (36%...