A5020800522- Lipid Membrane Structure and Behavior
- Bacterial Infections and Vaccines
- Lipid metabolism and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Cardiac Valve Diseases and Treatments
- Gastric Cancer Management and Outcomes
- Microbial Community Ecology and Physiology
- Metastasis and carcinoma case studies
- Fatty Acid Research and Health
- Gastrointestinal Tumor Research and Treatment
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Aortic Disease and Treatment Approaches
- Protein Structure and Dynamics
- Cardiac Structural Anomalies and Repair
- Gastrointestinal disorders and treatments
- Genomics and Phylogenetic Studies
- Extracellular vesicles in disease
- Genetic factors in colorectal cancer
- Aortic aneurysm repair treatments
- Bacterial biofilms and quorum sensing
- Microbial Fuel Cells and Bioremediation
- Cancer Research and Treatments
- Photoreceptor and optogenetics research
- Bacterial Genetics and Biotechnology
Kyoto University Institute for Chemical Research
2015-2024
Kyoto University
2015-2024
Kyoto Bunkyo University
2012-2024
Instituto de Investigaciones Químicas
2004-2024
Sasaki Institute
2013-2023
Ogikubo Hospital
2012-2019
Hiroshima City Asa Citizens Hospital
2015
Memorial University of Newfoundland
2014
Kure Medical Center
2003-2010
National Hospital Organization
2003-2010
After an intense bout of exercise, foam rolling is thought to alleviate muscle fatigue and soreness (ie, delayed-onset [DOMS]) improve muscular performance. Potentially, may be effective therapeutic modality reduce DOMS while enhancing the recovery performance.To examine effects as a tool after exercise protocol through assessment pressure-pain threshold, sprint time, change-of-direction speed, power, dynamic strength-endurance.Controlled laboratory study.University laboratory.A total 8...
Shewanella livingstonensis Ac10, a psychrotrophic gram-negative bacterium isolated from Antarctic seawater, produces eicosapentaenoic acid (EPA) as component of phospholipids at low temperatures. EPA constitutes about 5% the total fatty acids cells grown 4 degrees C. We found that five genes, termed orf2, orf5, orf6, orf7, and orf8, are specifically required for synthesis by targeted disruption respective genes. The mutants lacking showed significant growth retardation C but not 18...
ABSTRACT Bacteria secrete extracellular membrane vesicles (EMVs). Physiological functions and biotechnological applications of these lipid nanoparticles have been attracting significant attention. However, the details molecular basis EMV biogenesis not yet fully elucidated. In our previous work, an N-terminus-substituted FAAV peptide labeled with nitrobenzoxadiazole (NBD; nFAAV5-NBD) was developed. This can sense curvature a bilayer selectively bind to EMVs even in presence cells. Here, we...
ABSTRACT A recombinant protein expression system working at low temperatures is expected to be useful for the production of thermolabile proteins. We constructed a low-temperature using an Antarctic cold-adapted bacterium, Shewanella sp. strain Ac10, as host. evaluated promoters proteins abundantly produced 4°C in this bacterium express foreign used 27 and broad-host-range vector, pJRD215, produce β-lactamase Ac10. The maximum yield was obtained when promoter putative alkyl hydroperoxide...
Extracellular membrane vesicles (EMVs) play an important role in various bacterial activities. EMVs have potential for use as vaccines, drug-delivery vehicles, platforms extracellular production of recombinant proteins, and so on. In this study, we newly isolated a cold-adapted bacterium, Shewanella vesiculosa HM13, which abundantly produces EMVs, characterized them, analyzed their cargo transport mechanism. S. from the intestine horse mackerel prospective host low-temperature secretory...
Shewanella violacea DSS12, a deep-sea bacterium, produces eicosapentaenoic acid (EPA) as component of membrane phospholipids. Although various isolates from the deep sea, such Photobacterium profundum SS9, Colwellia psychrerythraea 34H and strains, produce EPA- or docosahexaenoic acid-containing phospholipids, physiological role these polyunsaturated fatty acids remains unclear. In this article, we illustrate importance EPA for high-pressure adaptation in strain DSS12 with help an...
1-Acyl-sn-glycerol-3-phosphate acyltransferase (designated as PlsC in bacteria) catalyzes the acylation of lysophosphatidic acid and is responsible for de novo production phosphatidic acid, a precursor synthesis various membrane glycerophospholipids. Because an integral protein, it generally difficult to solubilize without causing its inactivation, which has been hampering biochemical characterization despite ubiquitous presence physiological importance. Most studies have carried out using...
Peritoneal metastasis is one of the most frequent causes death in several types advanced cancers; however, underlying molecular mechanisms remain largely unknown. In this study, we exploited multicolor fluorescent lineage tracking to investigate clonality peritoneal mouse xenograft models. When was induced by intraperitoneal or orthotopic injection multicolored cancer cells, each peritoneally metastasized tumor displayed fluorescence regardless sites, indicating that it consists multiclonal...
Shewanella sp. HM13 is a cold-adapted Gram-negative bacterium isolated from the intestine of horse mackerel. It produces large amount outer membrane vesicles (OMVs), which are particles released in medium where cultured. This strain biosynthesizes single major cargo protein OMVs, fact that makes good candidate for production extracellular recombinant proteins. Therefore, structural characterization components vesicles, such as lipopolysaccharides, takes on fundamental role understanding...